Objective:To construct recombinant Bb-EmⅡ/3 vaccine of Echinococcus multilocularis and to investigate the expression efficiency of EmⅡ/3 antigen encoding gene in Escherichia coli BL21(DE3). Methods:EmⅡ/3 antigen gene was amplified by PCR. Then the gene was cloned into Escherichia coli-Bifidobacteria shuttle plasmid pGEX-1?T containing gluathione-S-transferaze(GST) gene to construct pGEX-EmⅡ/3. The recombinant plasmid was electroporated into Bifidobacteria bifidum(Bb) to construct rBb-EmⅡ/3 vaccine. The vaccine was identified with PCR and restriction-endonuclease digestion. The expression of pGEX-EmⅡ/3 was induced with isopropyl-?-D-thiogalactopyranosid(IPTG),and fusion protein EmⅡ/3/GST was examined by SDS-PAGE and Western blot techniques. Results:1 759bp gene of EmⅡ/3 was successfully amplified by PCR and cloned into pGEX-1?T by restriction analysis. rBb-EmⅡ/3 vaccine was successfully constructed according to PCR and restriction-endonuclease digestion.It was demonstrated with SDS-PAGE and Western blot that the fusion protein EmⅡ/3/GST were expressed in E.coli,BL21. Conclusions:rBb-EmⅡ/3 vaccine of Echinococcus multilocularis is successfully constructed. The plasmid pGEX-EmⅡ/3 was highly expressed in E.coli in fused form with GST and this kind of protein shows specific antigenicity according to western blot.

Objective To observe the effect of pelvic floor muscle rehabilitation training combined with Buzhong Yiqi Pill at ultra-early stage or early stage on the recovery of postpartum pelvic floor muscle function, to explore the feasibility of intervention of postpartum rehabilitation training at ultra-early stage. Methods 314 patients were randomly divided into the ultra-early stage group and the early stage group, 157 cases for each group. Patients in the ultra-early group were guided to give Kegel exercise and the biofeedback electrical stimulation therapy in the seventh week combined with Buzhong Yiqi pill in 1-2 days after delivery , patients in the early group were given Kegel exercise and biofeedback electrical stimulation therapy in the seventh week combined with Buzhong Yiqi pill. Effects of treatment two groups were observed. Results There were significant increased in pelvic floor muscle activity compared with six weeks and 2 days after delivery between two groups (P<0.05), and there were significant increased in the ultra-early group compared with the same period in the early group (P<0.05) . There were significant increased in good rate in 12 weeks after delivery compared with immediate time and 2 days after delidery (P<0.05), and more significant increased in the ultra- early group than that in the early group (P<0.05). There were significant decreased in urinary urgency, lumbosacral painand tenesmus, vaginal relaxation, and vaginal dryness rate in 12 weeks after delivery compared between two groups (P<0.05). Conclusion There were good effects in pelvic floor muscle rehabilitation training combined with Buzhong Yiqi Pill at ultra-early stage on the recovery of pelvic floor muscle function after delivery, and be worthy of clinical application.

Diabetic cardiomyopathy is one of the major causes of congestive heart failure in patients with diabetes.The histological changes of the diabetic cardiomyopathy can be accurately characterized by MR structure and functional imaging techniques,such as CINE imaging,MRS and T1 Mapping.These cardiac MRI techniques have been used to evaluate the treatment effect of diabetic cardiomyopathy.The progresses of MRI techniques in evaluating myocardium of diabetic cardiomyopathy were reviewed in this article.

Objective To investigate the clinical efficacy and safety of low-dose aspirin in prevention of lower extremity deep venous thrombosis after cesarean section. Methods from January 2016 to March 2017 in our hospital obstetrics and Gynecology cesarean section in 374 cases as the research object, using the method of random number table according to the proportion of 1:1 were divided into control group and observation group, each group of the 187 cases, the control group after cesarean section treated with routine comprehensive preventive measures, the observation group in the control group received the low-dose aspirin treatment. Observation group 2 postpartum in January the incidence of deep venous thrombosis, postoperative 12 h, postoperative serum 7 d two D- dimer (D-D), fibrinogen (FIB), activated partial thromboplastin time (APTT), prothrombin time (PT) and platelet count (PLT) and blood rheology index, statistics of the 2 groups the incidence of bleeding complications. Results Deep venous thrombosis in the observation group was significantly lower than the control group (P<0.05); the 2 group after 5D-D and FIB and 12 h after operation were significantly decreased (P<0.05), and the observation group and the control group in the same period were significantly decreased (P<0.05); 2 groups were PT, APTT, PLT showed no significant difference compared with the preoperative, and the observation group and the control group in the same period. There was no statistically significant difference ; 2 group 7 d after operation and high blood viscosity, whole blood viscosity, whole blood low shear viscosity, plasma viscosity and postoperative 12 h compared were significantly decreased (P<0.05), and the observation group with the same period compared with the control group decreased significantly (P<0.05); postoperative 7 d erythrocyte deformation index and postoperative 12 h were significantly increased (P<0.05), and the observation group and the control group in the same period compared significantly increased (P<0.05); the incidence of bleeding complications was not statistically significant 2 after the operation of group. Conclusion Hypercoagulability of low-dose aspirin helps improve puerpera after cesarean section, reduce blood viscosity, reduce the incidence of venous thrombosis, does not increase the incidence of bleeding, clinical application of good safety, which is worthy of clinical application.

Objective To investigate factors influencing satisfaction degree of patients receiving introvenous infusion in out-patient department in order to perfect nursing quality and improve the satisfaction degree of patients. Methods 900 patients receiving introvenous infusion in out-patient department were investigated with self-designed questionnaire and the results went through analysis. Results There were 886 effective responding questionnaires out of 900 with effective rate of 98.4%. The result indicated that the major factors which influenced degree of satisfaction with infusion included operation technique, service attitude and health education of nurses. Conclusions Increase of nurses' diathesis, enhancement of service quality, establishment of concept of "patient as center" are the preconditions to improve the satisfaction degree of patients receiving introvenous infusion in out-patient department.

Abstract: Objective ， To explore the relationship among job stress psychological contract and occupational adaptation in - . Methods newly hired policemen A total of 413 new police officers in a southwest province were selected as the research ， subjects using convenient sampling method. The Chinese version of Perceived Stress Scale the Psychological Contract ， Questionnaire and the Occupational Adaptation Scale were used to investigate their job stress psychological contract and occupational adaptation respectively. The effects of job stress and psychological contract on occupational adaptation was analyzed. Results ， （ ），（ ） The total scores of job stress psychological contract and occupational adaptation were 27.6±7.1 63.9±16.6 and （ ）， - 56.2±8.6 respectively. The work pressure of newly hired police officers was negatively correlated with occupational ［ （r） ，P< ］ adaptation correlation coefficient =−0.61 0.01 . There was a positive correlation between psychological contract and （r ，P< ） occupational adaptation =0.59 0.01 . The results of hierarchical regression analysis showed that after adjusting for ， ， ， ， ， confounding factors such as gender education level marriage fertility the stronger the sense of out of control in work stress （P< ）； ， the lower the level of occupational adaptation 0.01 the higher the score of psychological contract the higher the level of （P< ） occupational adaptation 0.01 . The psychological contract played a mediating role between job stress and occupational ， Conclusion - adaption accounting for 12.5% of the total effect. The job stress and psychological contract of the newly hired policemen can affect their occupational adaptation. Psychological contract plays a partial intermediary role in job stress on occupational adaptation.

Objective:To detect the effects of recombinant BCG-Em Ⅱ/3 vaccine on apoptosis of splenocytes in mice challenged with protoscoleces of Echinococcus multilocularis.Methods:BALB/C mice were immunized with rBCG-Em Ⅱ/3 vaccine by subcutaneous injection or intranasal inoculation.After 8 weeks of immunization,all the mice were challenged with 50 protoscoleces of Echinococcus multilocularis by intraperitoneal injection.On the 18th week after infection,mice were killed to separate splenocytes and stimulated with ConA for 16～18h,then apoptosis of splenocytes was detected by Annexin V-FITC staining methods.Results:Apoptotic rate of splenocytes in all groups with or without ConA stimulation was obviously lower than that of PBS group(P

Objective To observe the effect and mechanism of magnesium on nigral dopaminergic neurons in rats with Parkinson's disease(PD). Methods The PD rats were prepared by unilateral injection with 6-hydroxydopamine(6-OHDA) in the nigra and then divided into magnesium sulfate group,Madopar group,mixed group(magnesium sulfate+Madopar) and control (normal saline) group,and recived corresponding therapy by gastric perfusion for 28 d.The behavior change was observed. At the damage side,the number of tyrosine hydroxylase(TH) positive neurons in nigra was detected by immunohistochemistry; the activities of superoxide dismatase(SOD),glutathione peroxidase (GSH-Px) and the level of malondialdehyde(MDA) in striatum were measured by biochemistry method,the expression of caspase-3 mRNA and the protease of nuclear factor(NF)-?B P65 in nigra were detected by RT-PCR and Western Blot. Results After treatment,the stable contralateral rotation was found only in mixed group. Compared with other groups,the number of TH positive neurons in mixed group was significantly increased (allP

DNA microarray is the first studied and the most matured form of the bio-chip, which generated huge amounts of data in a number of valuable hidden information. Scientists have attached more importance to the exploration and development for the analysis of these data. At present, many methods have been used for large-scale gene expression data mining, such as statistical analysis, cluster analysis and self-organizing map.This paper reviews the existing methods for analyzing experimental data collected from and introduces some new analytical methods.

Objective To investigate the role of subdural puncture(SDP)in the diagnosis and treatment of subdural fluid collection in young children with purulent meningitis.Methods Totally 207