1.The relationship between expression of cyclooxygenase-2,vascularendotheliai growth factor and angiogenesis in hepatoceUular carcinoma
Dong-Mei ZOU ; Qing-Xu YANG ; Tong-Tong WU ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(08):-
Objective To investigate the significance of cyclooxygenase-2 (COX-2),vascular endothelial growth factor(VEGF) protein expression and angiogenesis in hepatocellular carcinoma(HCC).Methods Tissue sec- tions from 40 HCC patients were examined immunohistochemically for protein expression of COX-2 and VEGF.Mi- crovessel density(MVD) was counted by endothelial cells immunostained by anti-CD34 antibody.Results The ex- pression of COX-2 protein in well differentiated HCC was stronger than that in moderately differentiated HCC and poorly differentiated HCC(P
2.Thyroid hormone resistance with H435Y mutation in thyroid hormone receptor β: a pedigree analysis
Yan TONG ; Mei TU ; Wen WEI ; Yang CHEN ; Tong CHEN
Chinese Journal of Endocrinology and Metabolism 2015;31(1):55-58
[Summary] The genomic DNA was extracted from peripheral blood leukocyte of the patient with thyroid hormone resistance syndrome and 14 members of his family.The exons 1-10 of thyroid hormone receptor β (TRβ) gene were amplified by PCR.The products of PCR were sequenced directly to detect the gene mutation.The results showed that 3 members of this family were confirmed to have the C→T transition mutation at nucleotide 1 303 site within exon 10 of TRβ gene,and the missense mutation results in the substitution of histidine to tyrosine (H435Y).The heterozygous mutation may lead to the occurrence of thyroid hormone resistance syndrome.
4.Comparison between effects of mycophenolate mofetil and rapamycin on proliferation and apoptosis of cyst-lining epithelial cells of ADPKD patients
Tong ZHANG ; Lili FU ; Changlin MEI
Academic Journal of Second Military Medical University 2000;0(08):-
Objective:To investigate the effect of mycophenolate mofetil(MMF)on proliferation and apoptosis of cyst-lining epithelial cells in patients with autosomal dominant polycystic kidney disease(ADPKD),and to compare its effect with that of rapamycin(RAPA)in vitro.Methods: Primary cultured cyst-lining epithelial cells were treated with MMF and RAPA at different concentrations(0,0.005,0.05,0.5,5 ?g/ml)for 48 h or 72 h.The inhibitory effects of them on the cells were evaluated by MTT assay;the cell cycle distribution and apoptotic ratio were determined by flow cytometry.The morphological changes of cyst-lining epithelial cells were observed under transmission electron microscope.Results: Both MMF and RAPA significantly inhibited the proliferation of cyst-lining epithelial cells in a dose-and time-dependent manner.After 48 h treatment,the cells were blocked at S phase by MMF and at G0/G1 phase by RAPA.Both drugs induced cell apoptosis,with the maximal apoptotic rate being(5.53?0.27)% for MMF and(4.36?0.10)% for PAPA.Typical morphological changes of apoptotic cells were observed under electron microscope.Conclusion: MMF can effectively inhibit proliferation and induce apoptosis of cyst-lining epithelial cells,but its inhibitory effect is weaker than that of RAPA.
6.More efforts should be made to improve the level of diagnosis and treatment of infectious diseases of newborn infants.
Chinese Journal of Pediatrics 2003;41(12):881-883
Anti-Bacterial Agents
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therapeutic use
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Bacterial Infections
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diagnosis
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therapy
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China
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Communicable Diseases
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diagnosis
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therapy
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Cross Infection
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prevention & control
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therapy
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Granulocyte Colony-Stimulating Factor
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therapeutic use
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Granulocyte-Macrophage Colony-Stimulating Factor
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therapeutic use
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Humans
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Immunoglobulins, Intravenous
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therapeutic use
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Infant, Newborn
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Sepsis
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diagnosis
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therapy
8.Relationship between Gastric Acid and Stress Ulcer Produced by Water Immersion in Young Rats
jie, ZHAO ; wen, ZHANG ; xiao-mei, TONG
Journal of Applied Clinical Pediatrics 2006;0(19):-
Objective To establish the animal model with stress ulcer(SU) and probe the relationship between the gastric mucosal injury and lasting stress time,the dynamic structural changes of pH of gastric juice.Methods Twenty-four SD young rats were randomly divided into 4 groups:control group,45 min(groupⅠ),90 min(groupⅡ),3 h(groupⅢ)groups under water immersion restraint stress(WRS).The change of gastric mucosal ulcer index(UI),pH of gastric juice were observed.Results Acute gastric mucosal damage was induced by WRS,with the WRS time prolonged,UI increased gradually and pH of gastric juice remarkably decreased in experimental rats.UI was positively rela-ted with stress time(r=0.957 P
9.A case report of Kearns-Sayre syndrome.
Wei-hua ZHANG ; Qian TONG ; Dong-mei CHEN
Chinese Journal of Cardiology 2011;39(4):369-369
10.Optimization of Toxicity Attenuation Processing Technology of Amorphophallus sinensis Belval. by Orthogo-nal Test
Xinlu MEI ; Bin XU ; Feila XU ; Shuhua TONG ; Sanmin HE
China Pharmacy 2015;(22):3137-3139
OBJECTIVE:To optimize the toxicity attenuation processing technology of Amorphophallus sinensis Belval.. METH-ODS:With the overall score of taste stimulation and rabbit eye irritation of A. sinensis Belval. as the index,and based on the sin-gle factor method,orthogonal test was designed to investigate the the influences of the amount of saturated calcium hydroxide solu-tion,heating time and heating temperature on the processing effect,and verification tests as well as irratation comparison before and after processing were conducted. RESULTS:The amount of saturated calcium hydroxide solution and heating temperature had significant influence on the processing effect. The optimal processing technology was to add saturated calcium hydroxide solution 30 times as much as the amount of medicinal materials at 100 ℃ and heat it for 30 min. The verification tests showed overall scores of 8.05,8.44 and 8.37(RSD=2.5%,n=3). The average overall scores before and after processing were 0.12 and 8.54(n=10)re-spectively. CONCLUSIONS:The medicinal materials processed by the optimal technology have lower stimulation and irritation. The optimal technology is stable and reliable.