China ; Humans ; Occupational Exposure ; Occupational Health ; Private Sector

Objective: To study the effects of propofol of clinical concentration on isolated heart function and metabolism of oxygen free radicals in rats with obstructive jaundice. Methods: Thirty-six rats were randomly divided into 2 groups (n = 18): sham operated group (SO) and bile duct ligation group (BDL). The isolated working hearts were perfused for 30 min and 6 hearts were randomly selected from each group to measure the contents of basic MDA and SOD activity in the heart tissue. The rest animals of each group were randomly separated into 2 subgroups (n = 6): K-H solution perfusion group and propofol perfusion group. The isolated working hearts in K-H solution perfusion group were perfused with K-H solution for 40 min. The hearts in propofol perfusion group (SOP, BDLP) were perfused for 40 min with K-H solution containing 25 μmol/L propofol. After the hearts became stable, the following indices were recorded: HR, left ventricular developed pressure (LVDP), ± dp/dtmax, etc. The contents of MDA and SOD activity were measured after perfusion. Results: After perfusion with K-H solution for 30 min, the basic indices of heart function of BDL group were significantly lower than those of SO group (P<0.05). The indices significantly decreased after perfusion with propofol (P<0.05), but the rates of change of all indices were not different between SO and BDL groups. The contents of basic MDA in BDL groups were significantly higher than those of SO groups (P<0.05); the basic SOD activities in BDL were significantly lower than those of SO groups (P<0.05). After propofol perfusion, MDA content of BDLP group significantly decreased (P<0.05) and SOD activity significantly increased (P<0.05). Conclusion: Propofol at clinical concentration shows no significantly different effects on isolated working heart function of rats with and without obstructive jaundice, which might be related to the fact that propofol can protect SOD activity, lower MDA content and improve the metabolism of myocardial.

Electrocardiography ; Female ; Fetal Hypoxia ; complications ; Humans ; Infant, Newborn ; Myocardial Infarction ; etiology ; therapy ; Myocardium ; metabolism ; pathology ; Treatment Outcome

Objective: This work studied platelet-neutrophil aggregates (PNA) expression in patients with acute ST-segment elevation myocardial infarction (STEMI). Methods: Our research included in 2 groups: STEMI group, n=40 patients admitted in our hospital and Control group, n=30 normal subjects. Blood levels of PNA, platelet-monocyte aggregates (PMA) and platelet-lymphocyte aggregates (PlyA) were measured by lfow cytometry at admission and the results were compared between 2 groups. Results: Compared with Control group, STEMI group had similar blood levels of PlyA and PMA,P=0.245 andP=0.312; while increased blood level of PNA,P<0.05. Correlation analysis showed that platelet-leukocyte aggregates (PLA) level was positively related to CK-MB (r=0.4992,P<0.01) and cTnI (r=0.4365,P<0.01). Conclusion: Blood level of PLA, especially PNA was significantly higher in STEMI patients which suggested that increased PNA might be sued as a new index for AMI diagnosis in clinical practice.

BACKGROUND: MRI is generally considered as an important means to diagnose cervical disc herniation.OBJECTIVE: To explore the correlation between clinical manifestations of traumatic cervical disc herniation and MRI changes.DESIGN: A retrospective study.SETTING: Department of Orthopedics, Xinqiao Hospital of Third Military Medical University of Chinese PLA.PARTICIPANTS: We selected 123 patients with traumatic cervical disc herniation who came to the Department of Orthopedics, Xinqiao Hospital of Third Military Medical University of Chinese PLA, for treatment between June 1982 and June 2002. Their clinical manifestations fell into four types:of grade Ⅰ or Ⅱ, 14 of which lost motility with normal or slightly impaired icantly decreased or lost motility of the bilateral upper limbs with myotility of nificantly decreased motility and thignesthesia of the unilateral upper and impaired motility, decreased pain sensation of the lower limb on the opposite side, but with good myotility.METHODS: MRI examination was carried out in 123 cases of traumatic cervical disc herniation.MAIN OUTCOME MEASURES: The correlation between the clinical manifestations of 123 cases and MRI results.RESULTS: The clinical manifestations and MRI information of 123 cases verse-type herniation, clinically manifested as symmetric incomplete as central canal syndrome and significantly decreased or lost motility of the ripheral-type herniation, manifested as nerve root pain of unilateral side as well as pain sensation and thermesthesia on the opposite side.CONCLUSION: MRI typing suggests the segment, position and shape of disc herniation specified, and the 4 types of clinical manifestations indicate the consistency of anatomic location with the corresponding neural disorder.

Trials in public hospital reforms bear special significance for rational deployment of health resources and exploring the separation of hospital management from routine operations. The paper identified such setbacks in the present trials as easy technology measures, numerous models of separations, difficulty in setting pharmaceutical service fees, and inadequate governance. Authors also recommended such measures as searching for appropriate reforms for public hospitals locally, greater financial resources for completing the compensation mechanism of public hospitals, streamlining their management system for separation of management from operations, and improving governance. All these efforts are designed to enhance the public benefit nature of public hospitals.

Objective To investigate the role of peripheral blood lymphocyte apoptosis through Fas/ Fas ligand pathway in the development of viral myocarditis. Methods Twenty-five viral myocarditis (VMC) and 25 healthy children were chosen for study. The apoptosis percentage in peripheral blood iymphocytes was quantitatively examined, flow cytometry with Annexin V/PI double parameter was used. And the expressions of Fas and Fasl protein in peripheral blood lymphocytes were analyzed with flow cytometry. Results The percentage of apoptosis in peripheral blood lymphocytes in children with VMC [(0. 6976 ± 0. 1109)%] was significantly higher than that of healthy children [(0. 1288 ± 0. 1069) %] (t = 18.459, P ＜ 0.001). Moreover, the apoptosis cell percentage showed a significant positive correlation with the serum levels of myocardium enzyme (creatine kinase-MB, lactate dehydrogenase, creatine kinase, hydroxybutyrate dehydrogenase)(r =0. 817 ,P ＜0. 01 ;r =0. 785 ,P ＜0.01 ;r =0. 726 ,P ＜0. 01 ;r =0. 712 ,P ＜0. 01). The expression of Fas and Fasl protein in VMC patients(2. 8804 ± 0. 3020,4. 4496 ± 0. 4836) were higher than those of health children(2. 1300 ±0. 6200,0. 1238 ±0. 0384) ,which had significant difference(t = 17. 321 ,P ＜0. 001 ;t' =44. 577, P ＜ 0. 001). The apoptosis percentage of peripheral blood lymphocytes in children with VMC showed a significant positive correlation with the Fas and Fasl protein expression in peripheral blood lymphocytes(r =0. 972, P ＜ 0. 01; r = 0. 958, P ＜ 0. 01). Conclusion Apoptosis percentage of peripheral blood lymphocytes increases among children with VMC, probably because immune response decreased or it is related with the degree of myocardium damaged. Fas and Fasl play an important role in apoptosis of peripheral blood lymphocytes in children with VMC.

Objective To investigate the relationship between optic d isc hemorrhage and localized retinal never fiber layer defects (RNFLDs) in norma l-tension glaucoma. Methods In 83 patients with normal-tensi on glaucoma, the cumulative frequency and quadrantal distribution of optic disc hemorrhages were retrospectively analyzed. The neighboring relation between opti c disc hemorrhages and RNFLDs in a same quadrant and the changes of correspondin g retinal never fiber layer (RNFL) after the occurrence of optic disc hemorrhage s were observed by tridimensional photochromy of ocular fundus. Results (1) The occurrences and distribution of optic disc hemorrhages: 29 of 83(34.94%) patients (33 eyes) had totally 58 occurrences, including 39 in infer iotemporal area, 14 in superiotemporal area, and 5 in other area. (2) The relati onship of neighborhood between optic disc hemorrhages and RNFLDs: in the availab le tridimensional photochrome, 23 occurrences in 15 patients (16 eyes) were foun d with cuneiform RNFLDs in the same quadrant, in which 22 was near the border of cuneiform RNFLDs. (3) The changes of corresponding retinal never fiber layer (R NFL) after the occurrence of optic disc hemorrhages: the photochromes of 24 occu rrences in 20 patients (21 eyes) were kept well in the initial and the 2-year f ollow-up periods, while the changes of RNFL were found in each region correspon ding to the 19 occurrences (in inferiotemporal or superiotemporal area) in the i nitial photochrome, including 7 cuneiform defects with various sizes, and 12 dev eloped localized RNFLDs next to the initial hemorrhages in the optic disc. No ob vious localized RNFL corresponding to the other 5 occurrences (1 in inferiotempo ral, 1 in superiotemporal, and 3 in other areas) were found in the follow-up pe riod. Conclusion Optic disc hemorrhages in normal-tension gla ucoma occur mostly in inferiotemporal area, and secondly in superiotemporal area of optic disc, and the appearance of optic disc hemorrhages may suggest that th e localized RNFLDs would develop in the associated regions.

Objective To evaluate the therapeutic effect of tulobuterol tape on mild or moderate bronchial asthma in children less than three years old . Methods Sixty-two children with mild or moderate asthma were randomized to receive either tulobuterol tape(treatment group) or procaterol hydrochloride tablet(control group) on the basis of inhaled fluticasone propionate for 2 weeks. Symptom scores of asthma, frequencies of episode of wheeze, doses of inhaled ventolin as rescue drug and the incidence of adverse reactions were recorded. Results In the treatment group,the symptom scores during daytime in the treatment group were (2. 2 ±0. 9)/week and (0. 9 ±0. 5)/week after 1 and 2 weeks of treatment, respectively, which were significantly lower than that in the control group(3.4 ± 1. 1)/week and (1. 3 ± 0. 6)/week after 1 and 2 weeks of treatment, respectively) (P < 0. 05). The symptom scores during night in the treatment group was significantly lower than that in the control group after one week of treatment (1. 8 ± 0. 7) /week v. s. (3. 3 ± 0. 9) /week, P < 0. 05). The frequencies of episode of wheeze was significantly different between the two groups (2. 3 ±1.2 and 3. 6 ± 1.3 in the treatment and control groups, respectively (P < 0.05) .The doses of inhaled ventolin in the treatment group (2. 6 ±0.9 spray/week) was significantly lower than that in the control group (3.7 ± 0. 8) spray/week) (P < 0. 05). The incidence of adverse reactions in the treatment group was significantly lower than that in the control group (3. 12% v. s. 23. 33% ,x2 = 3. 89,P<0.05). Conclusions Tulobuterol tape is a safe and effective medication for the treatment of mild or moderate bronchial asthma in children less than three years old.