Objective:To study the effect of short exposure to high levels of fluoride on structure and the BMP-4 expression of ameloblasts of mouse molar and explore the possible formation mechanisms of dental fluorosis. Methods:32 4-day-old ICR mice were randomly divided into two groups. The experimental animals and the control animals were shared equal numbers in each group. The experimental animals were received a single intraperitoneal dose of 20 (n=8) and 10 (n=8) mg NaF/kg(body weight) respectively,equal doses of NaCl were given to the controls (n=8 for each group). The injected volume was kept constant (10 ?l/g). After 24 h all mice were sacrificed. HE and immunohistochemical stainings were used to observe the structural changes and the expression of BMP-4 in ameloblasts of mouse molar at different stages. SPSS 13.0 software package was used to analyze the data. Results:The early and late secretory/transitional ameloblasts were sensitive to a short-lasting but high dose of F-. The immunohistochemistry results demonstrated that in experimental animals,the expression of BMP-4 was weaker than that in control animals with the significant differences(P

Computer technology is one of the greatest achievements of the 20th Century. Its appearance not only brings a breakthrough development in various fields, but also brings promotion to the development of traditional Chinese medicine (TCM). TCM is a traditional medicine, and the modernization of TCM is urgent. Whether in the medical management or scientific research, the application of computer technology has penetrated into all levels. The following article described the contribution made by computer technology, pointed out possible problems and provided relevant thinking based on the application of TCM related systems and data mining technology.

Autoimmune Diseases ; classification ; Child ; Humans ; Immune System Diseases ; classification

Aged ; Aged, 80 and over ; Humans ; Lung ; physiopathology ; Male ; Middle Aged ; Pneumoconiosis ; physiopathology ; Respiratory Function Tests ; Respiratory Insufficiency

Adult ; Aged ; Aged, 80 and over ; Ceruloplasmin ; analysis ; Early Diagnosis ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; diagnosis

Objective To explore the changes of plasma catecholamines(CA)[including epinephrine(E) and norepinephrine(NE)] and atrial natriuretic peptide(ANP) in children with varying degrees of left ventricular ejection function(LVEF) in chronic heart failure(CHF),and establish natriuretic hormone factors as one of the diagnostic criteria of CHF.Methods Thirty-five patients with CHF and 35 children in good health were enrolled,and the concentration of plasma NE,E,ANP and the LVEF were determined.Results 1.The levels of plasma NE,E and ANP in 35 patients with CHF before treatment were significantly higher than those in control group(Pa

Objective: To develop an HPLC method for the simultaneous determination of eight saponins in alkali hydrolysate of total saponins from Pulsatilla chinensis. Methods: HPLC was performed on a Kromasil C18 analytical column (250 mm × 4.6 mm, 5.0 μm) at 35°C with MeOH-0.2% HCOOH solution as the mobile phase by gradient elution and the step gradients were as follows: 0-30 min, 70%-100% MeOH; The flow rate was 1.0 mL/min; ELSD gasification chamber temperature was 40°C; Gas pressure of carrier gas N2 was 350 kPa. Results: The linear response (the log values of peak areas with corresponding log values of sample introducing amounts) ranges were 0.799-4.568 μg for pulsatilla saponin D, 0.563-6.756 μg for hederagenin 3-O-β-D-glucopyranosyl-(1→4)-α-L-arabinopyranoside, 0.431-2.683 μg for pulsatilla saponin A, 0.894-7.826 μg for hederacolchiside A1, 0.643-7.504 μg for pulsatilla saponin F, 1.351-7.822 μg for oleanolic acid 3-O-β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl - (1→3)-α-L-rhamnopyranosyl-(1→2) - α-L-arabinopyranoside, 0.629-2.515 μg for oleanolic acid 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl - (1→2)-α-L-arabinopyranoside, and 0.698-2.794 μg for oleanolic acid 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside, respectively (n = 5). The average recoveries of the eight saponins were between 99.0% and 101.0%, and RSD values were less than 1.5%. Conclusion: The results demonstrate that the established method has the adequate accuracy and selectivity for the quality control of alkali hydrolysate of total saponins from P. chinensis.

Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; adverse effects ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; pathology ; Cisplatin ; administration & dosage ; adverse effects ; Deoxycytidine ; administration & dosage ; adverse effects ; analogs & derivatives ; Drug Chronotherapy ; Female ; Humans ; Leukopenia ; chemically induced ; Lung Neoplasms ; drug therapy ; pathology ; Male ; Middle Aged ; Nausea ; chemically induced ; Neoplasm Staging ; Neutropenia ; chemically induced ; Remission Induction ; Taxoids ; administration & dosage ; adverse effects

Humans ; Quality of Life ; Rhinitis ; Sinusitis

Objective: To study the effect of short exposure to high levels of fluoride on cellular structure and the DSP expression of odontoblasts of mice molars. Methods: 32 4-day-old ICR mice were randomly divided into two groups. The experimental animals were received a single intraperitoneal dose of 20 mg(n =8) andlO(n=8) mg NaF/kg( body weight) respectively. Equal doses of NaCl were given to the controls (n = 8 for each group). The injected volume was kept constant (10 μ/g). After 24 hours all mice were sacrificed. HE staining and immunohistochemical staining were used to observe the structural changes and the expression of DSP in odontoblasts of mouse molar at different stages. SPSS 13.0 software package was used to analyze the results. Results: The secretory odontoblasts distorted and lost its normal column contour. The immunohistochemistry results demonstrated that the expression of DSP was more intense than that in control group. Results of statistics analysis showed there were significant differences between the two groups(P<0.01). No remarkable differences were found in mature odontoblasts. Conclusion: Short exposure to high concentration of fluoride can enhance the expression of DSP in the secretory odontoblasts,and inhibit differentiation of the odontoblasts and matrix secretion. This lead to the abnormal development of dentinogenesis.