This paper introduced the current situation of medical informatics teaching faculty construction at home and aboard and pointed out the gap in faculty sources and education background between China and foreign countries.Meanwhile,this paper discussed on medical informatics teaching faculty construction plan from the aspects of broadening faculty sources,establishing ability quality standard,making personalized training program and constructing a scientific evaluation system.

Objective:To establish the determination method for total flavonoids in Cyperus rotundus and investigate the total fla-vonoids content in Cyperus rotundus from different habitats. Methods:Total flavonoids in Cyperus rotundus from different habitats were detected by ultraviolet spectrophotometry with rutin as the standard substance. Results:The absorbance of rutin had a good linear cor-relation with the concentration within the range of 0.064 3-0.642 6 mg·ml-1(r =0.999 1). The average recovery was 99.74%(RSD=2. 16%,n=6). The order of total flavonoids content in Cyperus rotundus from different habitats was Henan province >Sichuan province> Guangdong province >Anhui province > Halnan province >Shandong province. Conclusion:The method is easy and ac-curate, which can be applied in the quality control of Cyperus rotundus. The total flavonoids content in Cyperus rotundus from Henan province is the highest.

Objective: To study the dynamic expression of X-linked inhibitor of apoptosis protein (XIAP) and caspase-3 p17 after ischemia/reperfusion (IR) in rates. Methods: Thirty male Wistar rats were divided into sham-operated, ischemia 1.5 hours and reperfusion for 6, 12, 24, 48 and 72 hours (n = 5 in each group). A model of reperfusion after 1.5 hours of focal cerebral ischemia was established with the suture method. The expression of XIAP and caspase-3p17 in brains were observed respectively by immunohistochemical method. Results: A small amount of XIAP-positive cells were observed in the non-ischemic side in the sham-operated and IR groups, but no caspase-3 p17 positive cells were observed. The XI-APpositive cells expression began to increase (P = 0. 00) at 6-hour reperfusion, it reached to the peak at 12 hours and began to decrease at 24 hours, and then it gradually returned to normal at 48 hours (P = 0.549) as compared with the sham-operated group; a small amount of caspase-3 p17 positive cells were observed at 6-hour reperfusion, they reached the peak at 24 hours and remained for 72 hours, and they were significantly more than those at 6 hours after reperfusion (P = 0.004). Conclusion: Focal cerebral ischemia may induce the expression of apoptosis-inhibiting gene XIAP and apoptosis-promoting gene caspase-3 p17 to increase transiently, and the peak time of the former expression is earlier than that of the latter.

Objective:To discuss the importance and significance of training base establishment for clinical pharmacy of traditional Chinese medicine ( TCM) . Methods:The urgency and necessity of training base establishment for TCM clinical pharmacy was stated based on the status and problems of TCM clinical pharmacy, and the important significance of training base establishment of TCM clini-cal pharmacy was clearly put forward. Results:The establishment of TCM clinical pharmacy training base with the aim of training pro-fessional talents for TCM clinical pharmacy can provide clinical pharmaceutical care for patients with higher quality, which surely will promote the development of TCM clinical pharmacy and the pharmaceutical care level. Conclusion:It is extremely urgent and signifi-cant to set up TCM clinical pharmacy training base, which is the important topic needed to be solved in TCM clinical pharmacy work.

Electrocardiography ; Female ; Fetal Hypoxia ; complications ; Humans ; Infant, Newborn ; Myocardial Infarction ; etiology ; therapy ; Myocardium ; metabolism ; pathology ; Treatment Outcome

Bronchoalveolar Lavage ; Bronchopulmonary Dysplasia ; etiology ; therapy ; Humans ; Infant, Newborn ; Infant, Premature ; Infant, Very Low Birth Weight ; Intubation, Intratracheal ; adverse effects ; Male ; Positive-Pressure Respiration ; Respiratory Insufficiency ; etiology ; therapy ; Treatment Outcome

Objective To investigate the characteristics of respiratory impedance in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods The impulse oscillometry(IOS) and polysomnography (PSG) indices were measured in 52 patients with OSAHS, 13 patients with chronic obstructive pulmonary disease (COPD) and 17 healthy persons. Results The levels of R20, total respiratory impedence and central airway resistance in the patients with OSAHS were significantly higher than those in healthy persons (P

Objective To explore the impact of physical activities on adolescents' external problem behaviors and the mediating effect of self-esteem.Method The physical activity rating scale(PARS-3),Achenbach adolescent self rating scale,Rosenberg self-esteem scale and adolescent physical self-esteem scale were used to investigate 419 participants selected from middle school students in Beijing.Results Adolescents who participated in physical activities with moderate amount showed less external problem behaviors.Adolescents who participated in physical activities with large amount appeared to be of more self-esteem.Both self-esteem and body-esteem showed partially mediating effects between physical activities and disciplinary offence.Conclusion Adolescents conducting moderate amount of physical activities can enhance their self-esteem,and ultimately reduce the incidence of their external problem behaiviors.

Objective:To transfect human osteoprotegerin( o pg) gene into Cos-7 cells.Methods:The primers were designed b ased on the human opg cDNA sequence,total mRNA was isolated from 293 cells and RT-PCR was performed.The fragment of opg cDNA was inserted into pSecTag 2/B vector and sequenced by automatic sequence analyzer. The recombined plasmid was transfected into Cos-7 cells by Lipofectamine 2000 and OPG protein expressi on in Cos-7 cells was determined by immunohistochemistry and Western blot. Results:The sequence of opg cDNA from 293 cells obtained by RT- PCR was identical to the sequence provided by GenBank [gi:33878056]. The fragm ents of the recombinant plasmid digested with Hind Ⅲ,EcoR I and BamH Ⅰ and exa mined by 10 g/L agarose electrophoresis were consistent with predicted size.OPG over-expressing Cos-7 cells was selected and confirmed by immunohistochemistry and Western blot.Conclusion:Human opg gene may be transfect ed into Cos-7 cells.

Objective To investigate the role of interleukin-6(IL-6), interleukin-8(IL-8) and tumor necrosis factor-?(TNF-?) in the pathogenesis of secondary syphilis, and their relationship with RPR titers and clinical manifestations in secondary syphilis. Methods The serum IL-6, IL-8 and TNF-? were tested by ELISA. RPR titers of the patients were determined simultaneously. Histopathology was performed if skin lesions were present. Results Thirty three cases of secondary syphilis were studied. The average levels of serum IL-6, IL-8 and TNF-? were 78.7?82.5 pg/mL, 171.9?117.9 pg/mL and 115.7?67.9 pg/mL, respectively, in syphilis group, which were significantly higher in comparison with those in controls (P