China ; Humans ; Occupational Exposure ; Occupational Health ; Private Sector

Objective: To study the effects of propofol of clinical concentration on isolated heart function and metabolism of oxygen free radicals in rats with obstructive jaundice. Methods: Thirty-six rats were randomly divided into 2 groups (n = 18): sham operated group (SO) and bile duct ligation group (BDL). The isolated working hearts were perfused for 30 min and 6 hearts were randomly selected from each group to measure the contents of basic MDA and SOD activity in the heart tissue. The rest animals of each group were randomly separated into 2 subgroups (n = 6): K-H solution perfusion group and propofol perfusion group. The isolated working hearts in K-H solution perfusion group were perfused with K-H solution for 40 min. The hearts in propofol perfusion group (SOP, BDLP) were perfused for 40 min with K-H solution containing 25 μmol/L propofol. After the hearts became stable, the following indices were recorded: HR, left ventricular developed pressure (LVDP), ± dp/dtmax, etc. The contents of MDA and SOD activity were measured after perfusion. Results: After perfusion with K-H solution for 30 min, the basic indices of heart function of BDL group were significantly lower than those of SO group (P<0.05). The indices significantly decreased after perfusion with propofol (P<0.05), but the rates of change of all indices were not different between SO and BDL groups. The contents of basic MDA in BDL groups were significantly higher than those of SO groups (P<0.05); the basic SOD activities in BDL were significantly lower than those of SO groups (P<0.05). After propofol perfusion, MDA content of BDLP group significantly decreased (P<0.05) and SOD activity significantly increased (P<0.05). Conclusion: Propofol at clinical concentration shows no significantly different effects on isolated working heart function of rats with and without obstructive jaundice, which might be related to the fact that propofol can protect SOD activity, lower MDA content and improve the metabolism of myocardial.

Electrocardiography ; Female ; Fetal Hypoxia ; complications ; Humans ; Infant, Newborn ; Myocardial Infarction ; etiology ; therapy ; Myocardium ; metabolism ; pathology ; Treatment Outcome

Bronchoalveolar Lavage ; Bronchopulmonary Dysplasia ; etiology ; therapy ; Humans ; Infant, Newborn ; Infant, Premature ; Infant, Very Low Birth Weight ; Intubation, Intratracheal ; adverse effects ; Male ; Positive-Pressure Respiration ; Respiratory Insufficiency ; etiology ; therapy ; Treatment Outcome

Objective To investigate the role of peripheral blood lymphocyte apoptosis through Fas/ Fas ligand pathway in the development of viral myocarditis. Methods Twenty-five viral myocarditis (VMC) and 25 healthy children were chosen for study. The apoptosis percentage in peripheral blood iymphocytes was quantitatively examined, flow cytometry with Annexin V/PI double parameter was used. And the expressions of Fas and Fasl protein in peripheral blood lymphocytes were analyzed with flow cytometry. Results The percentage of apoptosis in peripheral blood lymphocytes in children with VMC [(0. 6976 ± 0. 1109)%] was significantly higher than that of healthy children [(0. 1288 ± 0. 1069) %] (t = 18.459, P ＜ 0.001). Moreover, the apoptosis cell percentage showed a significant positive correlation with the serum levels of myocardium enzyme (creatine kinase-MB, lactate dehydrogenase, creatine kinase, hydroxybutyrate dehydrogenase)(r =0. 817 ,P ＜0. 01 ;r =0. 785 ,P ＜0.01 ;r =0. 726 ,P ＜0. 01 ;r =0. 712 ,P ＜0. 01). The expression of Fas and Fasl protein in VMC patients(2. 8804 ± 0. 3020,4. 4496 ± 0. 4836) were higher than those of health children(2. 1300 ±0. 6200,0. 1238 ±0. 0384) ,which had significant difference(t = 17. 321 ,P ＜0. 001 ;t' =44. 577, P ＜ 0. 001). The apoptosis percentage of peripheral blood lymphocytes in children with VMC showed a significant positive correlation with the Fas and Fasl protein expression in peripheral blood lymphocytes(r =0. 972, P ＜ 0. 01; r = 0. 958, P ＜ 0. 01). Conclusion Apoptosis percentage of peripheral blood lymphocytes increases among children with VMC, probably because immune response decreased or it is related with the degree of myocardium damaged. Fas and Fasl play an important role in apoptosis of peripheral blood lymphocytes in children with VMC.

Background Studies showed that microRNA (miR)-375 suppresses the growth,apoptosis,migration and adhesion of tumor cells,and it plays a regulation to the changes of vascular endothelial growth factor (VEGF) in tumor tissue to arrest neovascularization.However,whether miR-375 intervenes the formation of new blood vessel in eyes is unelucidated.Objective This study was to explore the effects of miR-375 on human retinal capillary endothelial cells (HRCECs) function induced by hypoxia.Methods HRCECs were cultured using IMDM and divided into normal control group,CoCl2 model group,CoCl2 +miR-375 mimic group,CoCl2 +miR-375 mimic control group,CoCl2+miR-375 inhibitor group and CoCl2 +miR-375 inhibitor control group,and hypoxia cell models were created by adding 200 μmol/L CoCl2.MiR-375 and frizzled 4 (FZD4) small interfering RNA (siRNA) were transfected into the cells by 50 nmol/L miRNA lipidosome for 48 hours.The proliferation of the cells was detected by MTT assay;migrated number of the cells was examined by Transwell chamber assay;ELISA was employed to detect the concentrations of VEGF and VE-cadherin in the medium;the expression of β-catenin,cyclinD1,matrix metalloproteinase-2 (MMP2) and VEGF proteins were analyzed by Western blot;tube length of vessel formation was evaluated by Matrigel assay.Cultured cells were divided into normal control group,CoCl2 model group,CoCl2 +mock group and CoCl2 + FZD4 siRNA group,the relative expression of FZD4,a miR-375 targeted gene,was detected by luciferase reporter.Results The relative expression of miR-375 mRNA was significantly increased in the CoCl2 +miR-375 mimic group compared with the CoCl2 + miR-375 mimic control group and reduced in the CoCo2 + miR-375 inhibitor group compared with the CoCo2 + miR-375 inhibitor control group (t =-19.237,8.764,both at P＜0.01),with a higher transfected efficacy for miR-375.The cell proliferative fold,migrated cell number,VEGF and VE-Cadherin contents in the medium and the tube length were significantly different among the CoCl2 model group,CoCl2 +miR-375 mimic group,CoCl2 +miR-375 mimic control group,CoCl2 +miR-375 inhibitor group and CoCl2 +miR-375 inhibitor control group (F =24.324,26.776,14.113,19.225,15.040,all at P＜0.001),and those in the CoCl2 +miR-375 mimic group were evidently reduced in the CoCl2 +miR-375 mimic group compared with the CoCl2 +miR-375 mimic control group,while those in the CoCl2 +miR-375 inhibitor group were considerably elevated in comparison with the CoCl2 +miR-375 inhibitor control group (all at P＜0.01).The expressions of β3-catenin,cyclinD1,MMP2 and VEGF protein were significantly different among the normal control group,CoCl2 model group,CoCl2 +miR-375 mimic group,CoCl2+miR-375 mimic control group,CoCl2 +miR-375 inhibitor group and CoCl2 +miR-375 inhibitor control group (F=11.753,13.283,16.770,10.334,all at P＜0.001).In addition,the cell proliferative fold,migrated cell number and the tube length were significantly increased in the CoCl2 model group and CoCl2+mock group,and those in the CoCl2+FZD4 siRNA group were decreased in comparison with the CoCl2 +mock group (all at P＜0.05).Conclusions MiR-375 inhibits the growth,migration and tube formation ability of HRCECs in hypoxic status probably by regulating the activation of Wnt pathway via directly targeting FZD4.

Objective To investigate the relationship between optic d isc hemorrhage and localized retinal never fiber layer defects (RNFLDs) in norma l-tension glaucoma. Methods In 83 patients with normal-tensi on glaucoma, the cumulative frequency and quadrantal distribution of optic disc hemorrhages were retrospectively analyzed. The neighboring relation between opti c disc hemorrhages and RNFLDs in a same quadrant and the changes of correspondin g retinal never fiber layer (RNFL) after the occurrence of optic disc hemorrhage s were observed by tridimensional photochromy of ocular fundus. Results (1) The occurrences and distribution of optic disc hemorrhages: 29 of 83(34.94%) patients (33 eyes) had totally 58 occurrences, including 39 in infer iotemporal area, 14 in superiotemporal area, and 5 in other area. (2) The relati onship of neighborhood between optic disc hemorrhages and RNFLDs: in the availab le tridimensional photochrome, 23 occurrences in 15 patients (16 eyes) were foun d with cuneiform RNFLDs in the same quadrant, in which 22 was near the border of cuneiform RNFLDs. (3) The changes of corresponding retinal never fiber layer (R NFL) after the occurrence of optic disc hemorrhages: the photochromes of 24 occu rrences in 20 patients (21 eyes) were kept well in the initial and the 2-year f ollow-up periods, while the changes of RNFL were found in each region correspon ding to the 19 occurrences (in inferiotemporal or superiotemporal area) in the i nitial photochrome, including 7 cuneiform defects with various sizes, and 12 dev eloped localized RNFLDs next to the initial hemorrhages in the optic disc. No ob vious localized RNFL corresponding to the other 5 occurrences (1 in inferiotempo ral, 1 in superiotemporal, and 3 in other areas) were found in the follow-up pe riod. Conclusion Optic disc hemorrhages in normal-tension gla ucoma occur mostly in inferiotemporal area, and secondly in superiotemporal area of optic disc, and the appearance of optic disc hemorrhages may suggest that th e localized RNFLDs would develop in the associated regions.

Objective: This work studied platelet-neutrophil aggregates (PNA) expression in patients with acute ST-segment elevation myocardial infarction (STEMI). Methods: Our research included in 2 groups: STEMI group, n=40 patients admitted in our hospital and Control group, n=30 normal subjects. Blood levels of PNA, platelet-monocyte aggregates (PMA) and platelet-lymphocyte aggregates (PlyA) were measured by lfow cytometry at admission and the results were compared between 2 groups. Results: Compared with Control group, STEMI group had similar blood levels of PlyA and PMA,P=0.245 andP=0.312; while increased blood level of PNA,P<0.05. Correlation analysis showed that platelet-leukocyte aggregates (PLA) level was positively related to CK-MB (r=0.4992,P<0.01) and cTnI (r=0.4365,P<0.01). Conclusion: Blood level of PLA, especially PNA was significantly higher in STEMI patients which suggested that increased PNA might be sued as a new index for AMI diagnosis in clinical practice.

Objective To investigate the change trends of apparent diffusion coefficient (ADC) values under different b values of diffusion‐weighted imaging(DWI) in acute cerebral infarction .Methods The patients with acute celebral infarction were selected as the experimental group and the normal volunteers as the control group .The 5 different diffusion gradients were used ,including b=200 s/mm2 ,b=400 s/mm2 ,b=600 s/mm2 ,b=800 s/mm2 and b=1 000 s/mm2 .The two pairs of 20 cases of acute cerebral in‐farction and 20 healthy volunteers were performed the DWI scanning for detecting the ADC value under different b values .The ADC values in the two groups were statistically analyzed by using one‐way anova .Results In 5 different b values ,the ADC values in the case group was 0 .64 ,0 .59 ,0 .55 ,0 .47 and 0 .41 respectively ,which in the control group were 1 .04 ,0 .99 ,0 .92 ,0 .85 and 0 .78 re‐spectively ,the differences between the two groups were statistically significant ,moreover with the b value increase ,the ADC value had a decreasing trend(P< 0 .01) .Conclusion The ADC value in the patients with acute cerebral infarction is significantly de‐creased ,the ADC values are different under different b values of DWI ,moreover which shows the significantly negative correlation trend .