Salivary gland carcinoma such as adenoid cystic carcinoma (AdCC) is characterized by slow growth, diffuse invasion and lung metastasis, which determine the patient's prognosis. It is important to clarify an attractant molecule leading tumor cells to migrate. We examined the effects of stromal cell-derived factor (SDF) -1, a chemokine, on salivary gland carcinoma cell clone HSG and its subclone HSG-AZA3. SDF-1 promoted the invasion and migration of HSG and HSG-AZA3 cells dose-dependently. Immunocytostaining and RT-PCR indicated that HSG and HSG-AZA3 cells expressed SDF-1 receptor, CXCR4, both in protein and mRNA level, respectively. CXCR4 was present on the cell surface of HSG cells, and was downregulated by SDF-1 addition. Finally, we confirmed that CXCR4 was expressed in the tissue of AdCC. Our study suggests that SDF-1 and CXCR4 play a role in the migration of carcinoma of salivary gland origin.

We performed the Senning operation and pulmonary valvotomy in an 11-month-old baby with transposition of the great arteries (TGA) with an intact ventricular septum (IVS), and bicuspid pulmonary valvular stenosis associated with pulmonary hypertension (PH). Preoperative catheterization showed a pressure gradient (PG) between the left ventricle (LV) and main pulmonary artery (MPA) of 35mmHg, mean pulmonary artery pressure (MPAP) of 56mmHg, and pulmonary vascular resistance (PVR) of 11.2unit·m². The pure oxygen inhalation test showed a decrease in MPAP from 56 to 38mmHg, and a decrease in PVR from 11.2 to 5.5 unit·m². We could not perform lung biopsy to determine the surgical indications in terms of PH due to preoperative progressive congestive heart failure in this patient. Postoperative catheterization (28 days after the Senning operation) showed a decrease in PG between the LV and MPA to 8mmHg, and MPAP also decreased to 17mmHg. Two radical operations were possible in this patient. One was the arterial switch operation (ASO), and the other was the atrial switch operation, i. e. the Senning or the Mustard operation. We selected the Senning operation because there was the possibility that the new aortic valve might develop persistent stenosis and regurgitation after ASO and pulmonary valvotomy. The Senning operation may be an alternative in selected patients with TGA with IVS and pulmonary valvular stenosis.