1.Effects of Acupuncture-Moxibustion on the Function of Autonomic System (2)
Yosihumi YONESIMA ; Kenzi MIYAMURA ; Masaharu ARASHI ; Masahiro IWATA ; Takeshi MOTUO ; Kazushi NISIZYO
Journal of the Japan Society of Acupuncture and Moxibustion 1983;33(2):169-176
In a preceeding report, we studied the blood pressure response to electro-acupuncture, to situ stiumlation, and to spotted skin electrical stimulation. In this paper we report the effects of various acupuncture techniques on instantaneous heart rate and finger pulse wave height.
METHOD
Each of the ten healthy adults was seated in a chair. The following six techniques were separately applied to right VF20 Fengchi with a hard silver no. 3 needle for one minute.
1) To touch the skin with the needle tip lightly (Needle contact).
2) To tap in and take out the needle twenty times (Gentle tapping).
3) To insert the needle and leave it there (In situ).
4) To insert the needle and give it quick vertical motion (Sparrow pecking).
Heart rate tachogram and finger pulse wave height were recorded with a polygraph. In addtion to the four techniques, the responses to “Hand pressure” and to “No stimulation” were recorded and analysed.
RESULT
I. The common tendency of the responses to all the techniques is as follows:
a) The heart rate showed no marked change during stimulation, and then a significant decrease after stimulation.
b) The pulse wave height showed a significant decrease during stimulation, and then a significant increase after stimulation.
II. However, there are some differences in the response between the techniques:
1) Hand pressure: The heart rate showed a significant decrease during and after stimulation. The pulse wave height showed no marked change.
2) Needle contact: The heart rate showed no significant increase after the stimulation.
3) Gentle tapping: The heart rate showed a significant increase during stimulation, and then returned after stimulation.
4) In situ: The pulse wave height showed a significant increase shortly after stimulation through till the end.
5) Sparrow pecking: This technique evoked a typical response as described above (I-a, b).
2.Caspase-11 mediated inflammasome activation in macrophages by systemic infection of A.actinomycetemcomitans exacerbates arthritis
Okano TOKUJU ; Ashida HIROSHI ; Komatsu NORIKO ; Tsukasaki MASAYUKI ; Iida TAMAKO ; Iwasawa MARIE ; Takahashi YUTO ; Takeuchi YASUO ; Iwata TAKANORI ; Sasai MIWA ; Yamamoto MASAHIRO ; Takayanagi HIROSHI ; Suzuki TOSHIHIKO
International Journal of Oral Science 2024;16(3):499-514
Clinical studies have shown that Aggregatibacter actinomycetemcomitans(A.actinomycetemcomitans)is associated with aggressive periodontitis and can potentially trigger or exacerbate rheumatoid arthritis(RA).However,the mechanism is poorly understood.Here,we show that systemic infection with A.actinomycetemcomitans triggers the progression of arthritis in mice anti-collagen antibody-induced arthritis(CAIA)model following IL-1β secretion and cell infiltration in paws in a manner that is dependent on caspase-11-mediated inflammasome activation in macrophages.The administration of polymyxin B(PMB),chloroquine,and anti-CD11b antibody suppressed inflammasome activation in macrophages and arthritis in mice,suggesting that the recognition of lipopolysaccharide(LPS)in the cytosol after bacterial degradation by lysosomes and invasion via CD11b are needed to trigger arthritis following inflammasome activation in macrophages.These data reveal that the inhibition of caspase-11-mediated inflammasome activation potentiates aggravation of RA induced by infection with A.actinomycetemcomitans.This work highlights how RA can be progressed by inflammasome activation as a result of periodontitis-associated bacterial infection and discusses the mechanism of inflammasome activation in response to infection with A.actinomycetemcomitans.