1.Morphological descriptions of second and third instar larvae of Hypopygiopsis violacea Macquart (Diptera:Calliphoridae), a forensically important fly in Malaysia.
Ahmad Firdaus, M S ; Marwi, M A ; Syamsa, R A ; Zuha, R M ; Ikhwan, Z ; Omar, B
Tropical Biomedicine 2010;27(1):134-7
Hypopygiopsis violacea, a species of fly of forensic importance, was recovered from a corpse and described for the first time. The morphological structures of the second and third instar larvae of four specimens were examined using light microscope. Observations were focused on three main morphological characters: cephalopharyngeal skeleton, anterior and posterior spiracles. Cephalopharyngeal skeleton of second instar larva is darkly pigmented and without accessory sclerite below the mouth hook. The anterior spiracles of second and third instar larvae have 8-9 papillae each, arranged in a single row. The posterior spiracle of second instar larva has two spiracular slits with no thickening of peritreme. This differentiates it from the third instar, whereby the latter has three slits for each posterior spiracle. Cephalopharyngeal skeleton of third instar larva is heavily pigmented. An accessory sclerite is found below the hook part of third instar larva but is absent in second instar. Peritreme of the posterior spiracle of third instar larva is thick almost complete encircling a button. The intersegmental spines of the cuticular surface are dome-shaped and unicuspid. Third instar larva of this species is large with size approximately 15 mm long. These findings provide important identification features of immature stages of Hy. violacea which could be useful in forensic entomology.
2.Descriptions of the Larval Instars of Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae), a Species of Forensic Importance in Malaysia
Raja M. Zuha Raja Kamal ; Mohamed Abdullah Marwi ; John Jeffery ; Ahmad Firdaus Mohd. Salleh ; Wan Omar Abdullah ; Baharuddin Omar
Malaysian Journal of Health Sciences 2008;6(2):35-41
The anatomical structures of the first, second and third instars of Chrysomya rufifacies (Macquart) were examined by light microscopy. Observations were
documented on the three main characteristics; the cephalopharyngeal skeleton, anterior spiracle and posterior spiracle. The first instar larva bore cornuae of
fairly pigmented delineation with slim hypostomal sclerite and distinct dental sclerite. First instar did not have obscured anterior spiracle but posterior
spiracles were obscured with thin lining of opened peritreme. Intersegmental spines were evident. The second instar larva displayed a prominent anterodorsal
process approaching closer to hypostomal sclerite while upper margin of the dorsal cornua was slightly pigmented. Each anterior spiracle consisted of
nine to ten papillae, arranged in a single row. Peritreme of the posterior spiracle thick, opening at the end of peritreme was not wide and confined to
two spiracular slits. The third instar larva showed a prominent arch of the ventral cornua with broad and bold appearance. It approached the dorsal
cornua and became narrow at the incision median. The anterior spiracle consisted of a single row of nine to ten papillae while intersegmental spine
could be identified with one to three dark pigmented tips. A dark pigmented and wide periterime was observed confining three short and thick spiracular
slits while button was poorly pigmented. The most distinctive feature of this second and third instar larva was the slender, thorn-like tubercle with numerous
spined tips on the middle line segment of the body. These findings provide identification features of C. rufifacies larvae instars.
3.Butterflies (Lepidoptera: Nymphalidae) Associated with Pig Carrions in Malaysia
Heo Chong Chin ; Mohamad Abdullah Marwi ; John Jeffery ; Sofian-Azirun M ; Chen Chee Dhang ; Wan Omar Abdullah ; Ismarulyusda Ishak ; Baharudin Omar
Malaysian Journal of Health Sciences 2010;8(1):1-4
An entomological study was conducted in Tanjung Sepat, Selangor, Malaysia in May until September 2007 revealing
five species of butterflies (all from family Nymphalidae) were attracted to pig carcasses placed in an oil palm plantation. Euploea mulciber (Cramer 1777), Hypolimnas bolina (Linnaeus 1758), Elymnias hypermnestra (Linnaeus 1763), Mycalesis mineus (Linnaeus 1758) and Ypthima baldus (Fabricius 1775) came to the carcasses at different stages of decomposition. From this study, we know that nymphalid butterflies are attracted to carcasses but their roles are most probably unimportant in post-mortem estimation.