The ongoing coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is causing major damages in health and economies worldwide. The development of safe and effective vaccines for COVID-19 is of utmost importance yet none have been licensed to date. One of the strategies for vaccine development utilizes dendritic cells which express class I and class II human leukocyte antigen (HLA) molecules. These HLA molecules present the antigenic peptides to T cells which mediate the immune response. Thus, the study aimed to identify SARS-CoV-2 peptides with potential binding to HLA class I and class II molecules using different bioinformatics tools. SYFPEITHI and IEDB were used to predict epitopes for the most common HLA class I and II alleles among Filipinos. The top predicted epitopes were subjected to de novo and template-based molecular docking. Then, binding energies of the generated peptide-HLA complexes to putative T cell receptors were predicted using a homology modeling approach. Several predicted epitopes showed promising MHC and TCR binding, although results varied considerably between the prediction methods used. In particular, the results of de novo and template-based docking methods did not coincide, the latter of which generated complexes that more closely resemble typical peptide-HLA complexes. The results of this study will be validated by the next stage of the vaccine development project which is the in vitro assessment of the T cell responses elicited by dendritic cells pulsed with the candidate peptides.
COVID-19 ; SARS-CoV-2 ; Vaccines ; Molecular Docking Simulation

BACKGROUND: Many of the leading causes of mortality and morbidity in the Philippines are controllable with nature-based products, either as agents of intervention, or prevention, as nutritional supplements or for the control of side-effects of medications. The different R&D programs on nature-based products in the Philippines are usually conducted in isolation, or through silos. These often lead to products that are shortsighted, duplicate products, or products with minimal innovation, not readily applicable to population and environmental sustainability.

OBJECTIVE: The study aimed to draft an internationally benchmarked and integrated blueprint for a population health and environmental health-led nature-based product development and conservation for the Philippines.

METHODS: The methodology consisted of a review of literature; regional educational visits; and a series of consultative meetings with stakeholders.

RESULTS: The study resulted in a stakeholder-validated blueprint that assigns the Philippine Institute of Traditional and Alternative Health Care (PITAHC) to lead the way for Filipinos to produce more nature-based products that are of international quality and attuned with local health needs. The blueprint has identified "9 Optimizations" in the realization of this aspiration, including an expanded role for PITAHC, a national database, an ethical researchers list, and to produce at least five commercial products and 20 intellectual property rights within 5 years with an estimated total investment of approximately PhP 816 M.

BACKGROUND: With the COVID-19 pandemic and sudden transition to online learning, students experience academic difficulties, which are correlated to physical and mental health. The need for implementation of an online wellbeing intervention program for students may lead to better health and improved academic performance. OBJECTIVES: This study aims to evaluate the feasibility and effectiveness of a six-week online educational wellbeing program for University of Santo Tomas College of Rehabilitation Sciences (UST-CRS) students on their physical and mental health. METHOD: This will utilize a quasi-experimental one-group pretest-posttest design, with purposive sampling of at least 40 participants with no physical and/or mental condition. Participants’ eligibility will be screened using the Physical Activity Readiness Questionnaire and Counseling Center Assessment of Psychological Symptoms. Wix platform will be used to assess participants’ well-being, which encompasses psychological and physical activities including physical activity tracking, virtual group exercises, Positive Psychological Intervention, walking, motivational interviewing, mindfulness exercises, Acceptance and Commitment Therapy, and Internet-based cognitive behavioral therapy. The International Physical Activity Questionnaire and World Health Organization-Five WellBeing Index will be used as pre and post-tests. Descriptive statistics will be used to summarize data and inferential statistics for Paired t-tests or Wilcoxon. EXPECTED RESULTS The findings will include the participants’ responses on the feasibility of the physical and mental wellbeing components of the proposed online program. Results are expected to be beneficial to students and university personnel for promoting a conducive learning environment and may be recommended for college-wide online program implementation.
online ; feasibility