Objective To study the change of oxidative stress in the formation of NASH in rats induced by high-fat diet,and observe the preventive and therapeutic effect of TFHL on NASH and explore its mechnism.Methods 110 rats were randomly divided into the normal group(40 rats),the model group(40 rats),the A.Nattermam group(10 rats) and high dosage and low dosage TFHL(10rats each).The normal rats were fed with the standard feedstuff the other rats fed with the high-fat diet.At the time of model-making,the rats in the A.Nattermam were fed the A.Nattermam suspension by daily gavage by 195.4mg/(kg?d),the rats in the high dosage and low dosage TFHL group were fed TFHL by daily gavage in 250、125mg/(kg?d),the rats in the normal and model groups were fed isometrical distilled water by daily gavage.At the ends of 2nd,4th,8th weeks of the experiment,10 rats in the nomal and model were killed respectively,at the end of experiment,all the rest were killed.pathological changes of liver tissues were observed,the levels of serum ALT,AST,the amount of TG,CHOL of livers tissue,the amount of MDA of serum and liver,the activity of OD、GSH-PX、T-AOC were measured.Results 1.the rats were fed with high-fat diet from 2 to 12w,the fatty degeneration of liver tissues were gradually serious,the amounts of TG,CHOL of livers were gradually increased.at 12th w the inflammatory cells in livers infiltrated obviously,the serum ALT,AST were obviously increased,it apeared a progressive process of NASH.2.from 4th w,after feeding with high-fat diet,the amounts of MDA of serum and liver were gradually decreased,the activity of SOD were gradually increased,the amount of T-AOC were gradually decreased as well and,increase or decrease with the development of inflammation.3.the inflammation degree of liver,the amount of liver CHOL and the serum ALT,AST in FMCL groups were lower than those in the corresponding model group;the amounts of MDA of serum and liver were decreased,the activity of SOD,GSH-PX and T-AOC were inceased than those in the corresponding model group,their oxidationresisting activity was better than those in the A.Nattermam group.Conclusions oxidation stress/Lipid Peroxidation plays an important role in the occurrence and development of NASH,TFHL can effectively treat and prevent the development of NASH,obviously ease oxidation stress,decrease Lipid Peroxidation of NASH,markedly enhance oxidationresisting abilily of body,which is the important mechanism of prevention and treatment for NASH.

Objective To investigate the mechanism of phenylethanoid glycosides of pedicularis muscicola Maxim ameliorating high altitude memory impairmentby activating mTOR signal pathway.Methods 60 clean male Wistar rats were randomly divided into normoxic control group, hypoxia group, PhGs low, medium and high dose groups(50, 200, 400 mg/kg by oral administration).Normoxic control and hypoxia groups were administered with sterile injection water for 7 days.On the fourth day of drug treatment, hypoxia and PhGs groups were exposed to a specially designed animal decompression chamber, which simulated 7 500 m high altitude environment.The expression levels of mTOR, P70S6K and 4E-BP1 mRNA in hippocampus were detected by SYBR Green real-time PCR.The expression levels of p-mTOR, p-P70S6k and p-4E-BP1 protein in hippocampus were detected by Western blot.Results For hypoxia group rats, mTORand P70S6k mRNA repression, p-mTOR and p-P70S6K protein repression were respectively decreased by 22.50%, 26.00%, 42.28% and 11.70%(P<0.05, P<0.01), 4E-BP1 mRNA repression and p-4E-BP1 protein repression were respectively increased by 41.28%, 111.86%(P<0.01) in comparison tonormoxic control group.Compared with hypoxia group,for PhGs low dose group rats, 4E-BP1 mRNA repression and p-4E-BP1 protein repression were respectively decreased by 77.33% and 82.4%(P<0.01), p-P70S6K protein repression was increased by 32.53%(P<0.01).For PhGs medium, high dose groups, mTOR and P70S6k mRNA repression,p-mTOR and p-P70S6K protein repression were respectively increased by 64.56%, 60.76%;14.86%, 20.27%;65.12%, 94.17% and 56.63%, 78.31%(P<0.01), 4E-BP1 mRNA repression and p-4E-BP1 protein repression were respectively decreased by 72.67%, 71.57% and 57.6%, 40%(P<0.01).Conclusion Phenylethanoid glycosides of Pedicularis muscicola Maxim can ameliorate high altitude-induced memory impairment.This protective mechanism may due to the activation of mTOR signal pathway.