Objective To evaluate the diagnostic value of CT guided percutaneous transthoracic core needle biopsy (TCNB). Methods CT guided TCNB were performed on 121 cases who had suffered from pulmonary diseases. Cook QC 18G, 19G, or 20 gauge needles were used. The diameter of the pulmonary lesions was ranged from 0.8cm to 9.5cm, mean (3.4?1.9)cm. Postbiopsy complications were observed by routine CT scan. Results According to the Westcott's method, the final diagnosis of 86 cases of malignancy and 35 cases of benignancy had been established. Seventy nine malignant and 32 benign ones could be accurately diagnosed by TCNB. The overall diagnostic accuracy was 91.7%(111/121). The sensitivity of TCNB in the malignancy was 91.9%(79/86) with 7 cases of false negative, and the specificity was 100%. Seventy cases of malignancy could be made definitely. The sensitivity of benignancy was 91.4%(32/35). Complication of pneumothorax in 22 cases (18.2%) and pulmonary hemorrhage in 19 cases (15.7%) resolving spontaneously. Conclusions CT guided TCNB is a safe, reliable method with high accuracy in diagnosis and less complications, especially for non lung cancer malignancy and benign lesions.

Objective To present the experiences in using the tubularized incised plate urethroplasty (Sondgrass procedure) for hypospadias repair. Methods Tubularized incised plate urethroplasty was performed for 43 cases of hypospadias including 35 distal penile shaft and 8 penoscrotal hypospadias.9 children have had a history of failed urethroplasty.Dorsal plication was carried out for 10 chordees.Bladder drainage was maitained for 10 days.After withdrawing the drainage,regular urethral dilatation was conducted. Results The patients have been followed up for 4～11 months and a good functional neourethra with a vertical slit like meatus was observed.There has been no chordee or stricture.5 patients had urethro cutaneous fistula,4 have been corrected. Conclusions Tubularized incised plate urethroplasty can be used in distal and proximal hypospadias repair including those with chordee and is especially indicated for patients without enough penile skin after failed urethroplasty.

Objective To evaluate diuretic renogram (DR) in the assessment of therapeutic effect on dilated upper urinary tract in children. Methods A total of 54 children with 66 sides of dilated upper urinart tract undergone surgery had been followed up by DR examination. Results According to the renal blood perfusion rate (BPR),the dilated upper urinary tract was divided into 3 catagories,the minor,medium and the major.In minor and medium catagories (49 sides),the renogram on 37 sides changed from obstructive pattern (no excretory phase) to reduced excretion ones which slopped downward obviously on frusemide administration (dilated nonobstructed pattern).The renogram had no change on 12 sides.In the major catagory (17 sides),BPR changed from 23.8?3.5% preoperatively to 33.4?6.4% postoperatively ( P

OBJECTIVE：To study the anti-asthmatic effects of artesunate on the durg-induced asthmat.METHOD:Drug-induced asthmatic model of guinea-pig. RESULTS:Using nubilous inhalation or intragastric administration,artesunate significantly inhibited the guinea-pig model asthma in a dose-dependent manner.It's ED50 were (99.95±7.69)mg.L-1(n=4) and (15.64±1.25)mg.kg-1.d-1(n=4) respectively. CONCLUSION:artesunate showed anti-athmatic effect in guinea-pig asthmatic model.

By analyzing the educational state in Video Science in medical colleges,combining with work practice,it puts forwards the teaching reform thought of it,aiming to jointly exploring and cultivating the clinical medical talents fit to the new TCM,combination of TCM and western medicine in the modern medical Video Science development.

Objective To observe the pathological changes of major organs in rats with acute Dysosma versipellis poisoning and investigate the toxic mechanismand the injuries of target tissues and organs. Methods Forty Sprague-Daw ley (SD) rats were random ly divided into three experimental groups, which were given the gavage with 0.5, 1.0 and 2.0LD50 doses of Dysosma versipellis decoction, and one con-trol group, which was given the gavage with 1.0LD50 dose of normal saline. The rats were sacrificed 14 days after Dysosma versipellis poisoning and sam ples including brain, heart, liver, lung, and kidney were taken. After pathological process, the pathological changes of the major organs and tissues were observed by light microscope and electron microscope. The experimental data were statistical analyzed by x2 test. Results The observations of light microscopy: loose cytoplasmof neurons with loss of most Nissl bodies;swelling of m yocardial cells with disappearance of intercalated disk and striations;hepato-cellular edema with ballooning degeneration; and swelling epithelial cells of renal proximal convoluted tubule with red light coloring protein-like substances in the tube. The observations of electron microscopy:the structures of cell mem brane and nuclear mem brane of neurons were destroyed;cytoplasmof neurons, obvious edema;and most organelles, destroyed and disappeared. The mortalities of rats after acute poi-soning of the four groups increased with doses (P<0.05). Conclusion Acute Dysosma versipellis poisoning can cause multi-organ pathological changes. There is apositive correlation between the toxic effect and the dosage. The target tissues and organs are brain (neurons), heart, liver and kidney.

Objective To investigate the relationship between the the expression of SOCS-1/3 in the peripheral blood mononuclear cells (PBMCs) and interferon therapeutic response in patients with chronic hepatitis B.Methods 50 patients with chronic hepatitis B were given interferon therapy.After 24 weeks treatment,according to biochemical and virologic response,they were divided into two groups:response and non response group.HBV-DNA was detected by PCR.The SOCS-1/3 mRNA levels were measured by RT-PCR.Results The expression of SOCS-1 in the PBMCs of response group after 24 weeks treatment was significantly higher than that before interferon therapy (P ＜ 0.05).The expression of SOCS-3 in the PBMCs of response group after 24 weeks trentment was significantly lower than that before interferon therapy (P ＜ 0.05).In non response group,there were no significant differences in the expression of SOCS-1/3 (P ＞ 0.05).Conclusion The increase expression of SOCS-1 mRNA and the decrease of SOCS-3 mRNA in the PBMCs in chronic hepatitis B patients received 24 weeks interferon-therapy probably is relevant to the IFN-α antiviral effect.

The effects of Lipoid of Mujingzi〔LP,Fruits of vitex neguando L Var cannabifo- lia (Sieb et Zucc) Hand mazz〕 on Smooth muscles are reported. The results were as follows:① In the isolated perfused mice lung-bronchi, LP caused significant increase of perfusion medium dropping through the lung-trachea, and the effect of LP was dose-dependent; ACh (Acetyl-choline)-induced contraction of the isolated mice trachea smooth muscles could be relaxed by LP. ② When LP was given introperitoneally(100~ 300 mg?kg-1) into guinea pig, the incubationperiod of asthma induced by acetycholine together with histamine was prolonged.The optimum dilated effect of LP on smooth muscles of trachea in mice can be concluded as the results of synergistic effects by 4 compositions from LP (Oleic acid,Linoleic acid, Palmitic acid and Stearic acid).

Objective To evaluate inspiratory and expiratory high resolution CT (HRCT) lung findings in normal adults and set up inspiratory and expiratory HRCT criterion for further clinical diagnosis and differentiating diagnosis. Methods Sixty-four healthy adults underwent inspiratory and expiratory lung HRCT scanning. The lung density and slice area in upper, middle and lower fields were measured in inspiratory and expiratory phases respectively. Results The lung density in inspiratory phase was (-795?58)HU, the lung density in expiratory phase was (-706?59)HU, the inspiratory expiratory value difference was (90?43)HU, and the expiratory inspiratory area ratio was 0.72? 0.14. Conclusion HRCT will be a new method for lung function testing in future.

Objective To observe the morphological structures of WHBE rabbit brain in vivo based on 3.0 T magnetic resonance imaging system (MRI), accumulate the basic biological data of WHBE rabbit brain imaging, and provide a background information to further expand the WHBE rabbit application.Methods Nine healthy adult male WHBE rabbits were intravenously anesthetized with 3% pentobarbital sodium.3.0 T MRI plus rabbit brain dedicated coil was used to perform routine transverse and sagittal scans, and the size of brain structures were measured.Results MRI scanning can be successfully performed to obtain sagittal and transverse T2WI or T1WI images of WHBE rabbit brain in vivo, and can be clearly observed the basic structures of WHBE rabbit brains in vivo, such as olfactory bulb, cerebrum, cerebellum and pituitary gland.In addition, high signal was found in the hippocampus of the left and right temporal lobes in 4 rabbits with T2WI, but also low signal appeared in the corresponding regions in T1WI, and the others were not abnormal.Meanwhile, the reference data of frontal lobe, hippocampus, cerebrum, lateral ventricles, pituitary gland and other related anatomical structures were also obtained.Conclusions Using the 3.0 T magnetic resonance imaging system and rabbit brain coil,the morphological and anatomical structures of rabbit brain can be clearly observed, and the basic imaging data of WHBE rabbits brain have been established preliminarily.