OBJECTIVE:To establish the quality standard for Mian medicine Indigofera stachyoides. METHODS:Medicinal properties and microscopic characteristics were identified;TLC was used for the qualitative identification;HPLC was conducted for content determination of epicatechin and 2α,3α-epoxy-5,7,3′,4′-tetra-hydroxy-flavan-(4β→8)-epicatechin(reference A):the col-umn was Comatex TM C18 with mobile phase of acetonitrile- water at a flow rate of 1.0 ml/min,and detection wavelength was 210 nm,column temperature was 30 ℃,injection volume was 5 μl. RESULTS:It showed clear microscopic identification map. I. stachyoides TLC had clear spots and well separated. The linear range was 0.274 5-4.392 0 μg (r=0.999 6) for epicatechin,and 0.103 0-1.648 3 μg for reference A(r=0.999 4),RSDs of precision,stability and reproducibility tests were lower than 3%,recov-eries were 99.76%-104.82%(RSD=2.42%,n=6) and 97.98%-104.99%(RSD=2.75%,n=6) respectively. CONCLUSIONS:The established standard can be used for the quality control of I. stachyoides.

Objective To establish a new gas chromatography (GC)method coupled with ultrasound-assisted extraction (UAE)and solid-phase microextraction (SPME)for rapid determination of seven kinds of organophosphorus pesticide residues and three kinds of pyrethroid pesticide residues in 13 Miao ethnomedicines produced in Guizhou province.Methods Japanese Honeysuckle Flower (Flos Lonicerae Japonicae,Jinyinhua)was the optimization object.SPME was used directly for enrichment of extracts obtained by ultrasonic-assisted solvent.Then gas chromatography-electron capture detector was used for the determination.Results Under optimum conditions,seven kinds of organophosphorus pesticides showed a good linear relationship at the range from 5 to 500 μg/L,and three kinds of pyrethroid pesti-cides did at the range from 2.5 to 250 μg/L.The recovery was from 85.6 % to 112.7 % with the RSD 3.2% ~9.8%.The limit of detection was from 0.02 to 0.33 μg/L.Conclusion UAE-SPME-GC established is an easy,environmental-friendly and rapid method for simultaneous determination of residual amount of 10 organophosphorus and pyrethroid pesticides in Miao ethomedicines.This method with directly enriching and sampling,was a simplified sample pretreatment procedure without purification process.