Objective:To evaluate the understanding and success rate in diagnosis and treatment of adrenal cortical tumor in children.Methods:6 cases were analyzed retrospectively about diagnosis,treatment,pathomorphological changes and prognosis.Results:6 cases of adrenal cortical tumor were treated surgically,which include 4 of cases Cushings syndrome,4 cases developed HBP,in 1 case developed hyperglycemia and in another case had hypokalemia developed.1 patient died of Ⅲ AVB(atrial ventricle block,AVB) during operation.Conclusion:Early diagnosis and early surgical interventions are crucial for treatment.Perioperative management plays an important role in improving prognosis.

Bacterial Infections ; diagnosis ; etiology ; therapy ; Brain Injuries ; etiology ; China ; Humans ; Infant, Newborn ; Intensive Care, Neonatal ; Respiration, Artificial ; adverse effects ; Respiratory Distress Syndrome, Newborn ; diagnosis ; therapy ; Respiratory Insufficiency ; diagnosis ; therapy ; Respiratory Tract Infections ; diagnosis ; etiology ; therapy ; Systemic Inflammatory Response Syndrome ; diagnosis ; therapy ; Ventilators, Mechanical ; adverse effects

Objective:To study effect of transcatheter occlusion and minimally invasive surgical occlusion in treating secundum atrial septal defect. Methods:Patients with secundum atrial septal defect were collected and randomly divided into observation group given transcatheter occlusion and control group received minimally invasive surgical occlusion. Then operation related index and and heart function index were observed. Results: there were no differences in operation related index between two groups(x2=1.039, P>0.05);operation success had negative correlation with age and positive correlation with LEVF (t=7.832, P<0.05) and no correlation with sex(t=0.892, P>0.05);in patients older than 45, the successful case, LEVF and LVEDD of observation group were significantly higher than control group(t=5.372, t=5.382;P<0.05). Conclusion:transcatheter occlusion and minimally invasive surgical occlusion can achieve ideal therapeutic efficacy;transcatheter occlusion has positive clinical value in patients over 45 years old for its advantages of small operation wound.

Objective To assess and quantitatively analyze the comprehensive benefits of improvement of drinking water quality in Hetian county of Xinjiang Methods The incidences of water borne infectious diseases(dysentery,hepatitis,typhoid and cholera) during 1992~1998 and the effects of health education were reviewed The decreases of the costs for drinking water transportation and the saving of medical expenses were analyzed quantitatively Results 95% of the total population were benefited from the improvement of drinking water quality The incidence rates of water borne infectious diseases except dysentery revealed a decreasing trend during 1992~1998 in Hetian county The benefited population presented higher levels of sanitary knowledge and good sanitary habits By the end of 2000,the accumulative investment for water improvement reached up to 45 50 million yuan,the saving of the costs for drinking water transportation and medical expenses was from 18 64 million yuan to 42 46 million yuan and 6 60 million yuan respectively,the benefit of building water plants and the development of peasant family economy was 2 91 million yuan and 35 56 million yuan respectively The totoal direct economic benefit reached from 67 31 million yuan to 87 52 million yuan Conclusion The project of improvement of drinking water quality revealed significant social and economic benefit,which had obviously exceeded the total investment

Objective To investigate the change of S-100B in serum in rats at varions times after exposure to different whole-brain irradiation doses and to explore the characteristics of the S-100B change in early radiation-induced brain injuries. Methods A model of brain radlation-induced injuries was estabhshed in the rat after whole-brain irradiation with a single dose of 2, 10, or 30 Gy of 4 MeV electron. The concentration of S-100B in serum was detected by means of enzyme-linked immunoserbent assay (ELISA) at different time points (1,6,12,24,3 days, 1 week, 1 month) after exposure. Results In terms of measuring time points, the serum concentration in all groups reached the fwst peak 6 hours after irradiation, declined after 12 hours, rose again after 24 hours, and started to decline again until the last time point (1 month), with the exception of the 2 Gy group, which reached the second peak at 3 days after irradiation. Conclusions The S-100B serum concentration was changed in the rats after whale-brain irradiation and these changes are positively related to the irradiation amount. It seems that the higher the dose, the higher the serum concentration and the differences between the dose groups are significant (via multiple regression models). The S-100B may be a valuable serum marker for the brain irradiation injury.

Colonic Polyps ; genetics ; pathology ; Colorectal Neoplasms ; genetics ; pathology ; CpG Islands ; DNA Methylation ; Genes, APC ; Genes, ras ; Humans ; Intestinal Polyps ; genetics ; pathology ; Microsatellite Repeats ; Mucin-2 ; Mucins ; analysis ; Mutation

Objective: To establish an androgen-independent human prostate cancer cell line model LNCaP-AI. Methods: LNCaP cells were cultured in absence of hormone for a long-term to establish LNCaP cell line LNCaP-AI, which can live without hormone. MTT, immunofluorescence and RT-PCR techniques were used to study the proliferation activity of LNCaP-AI cells and expression and secretion level of PSA by LNCaP-AI cells in absence of hormone. Results: After cultured for 3 months, LNCaP cells gradually became accustomed to the non-hormone condition, showing the characteristics of androgen-independent LNCaP-AI cell line. LNCaP-AI cells rapidly proliferated under the non-hormone condition and secreted PSA. However, PSA mRNA expression level in LNCaP-AI cells was 44% that of the LNCaP cells under hormone condition. Conclusion: Androgen-independent LNCaP-AI cell line may simulate the development of androgen-independence in prostate cancer cells and is an ideal model of androgen-independent prostate cancer cell line.

OBJECTIVETo explore the relationship of the classification of talus fracture and surgery methods with avascular necrosis.

METHODSFrom March 2009 to November 2013, 78 patients with talus fracture were treated, of them, 43 cases were followed up from 2 to 5 years. There were 27 males and 16 females, aged from 17 to 65 years old with the mean of 38.6 years. Thirty-nine cases had talar neck injury and 4 cases had talar body injury. Different treatments were performed according to different injury conditions. The time from injury to treatment was from 6 to 48 hours. The effect of classification of talus fracture and surgery methods on avascular necrosis was analyzed.

RESULTSIn 43 cases,19 cases occurred avascular necrosis, including talar neck fracture of type I in 2 cases, type II in 5 cases, type III in 5 cases, type IV in 5 cases and talar body fracture in 2 cases (combined with talar neck fracture). And 29 patients were treated with operation, there was no statistically significant differences in avascular necrosis with different operations.

CONCLUSIONCompared with talar body fracture, talar neck fracture is more easily to develop into avascular necrosis. In the 4 types of talar neck fracture, the possibilities of type III and IV were the most ones.

Adolescent ; Adult ; Aged ; Female ; Fracture Fixation, Internal ; methods ; Fractures, Bone ; classification ; surgery ; Humans ; Male ; Middle Aged ; Osteonecrosis ; surgery ; Talus ; blood supply ; injuries

The optimum conditions for the processing of Sparganium stoloniferum Buch-Ham. withvinegar were studied by L9(3)4 orthogonal experimental design, as guided by decrease of writhing rate inanalgesic test and anticoagulant activity. The results showed that the best processing conditions were stirfrying of the raw S. stoloniferum with the addition of 25 kg of vinegar per 100 kg of crude drug at 130℃for 10 min.

Objective To investigate the influence of coix seed triglyceride combined with transcatheter arterial chemoembolization (TACE) therapy on AFP, CD4﹢CD25﹢regulatory T (Treg) cells and cellular immune function in patients with advanced primary hepatocellular carcinoma (HCC). Methods A total of 50 patients with inoperable HCC, whose imaging examination showed no distant metastasis, were divided into the study group (n=25) and the control group (n=25). Coix seed triglyceride together with TACE was employed for the patients of the study group, while only TACE was adopted for the patients of the control group. For the patients of the study group, transcatheter hepatic artery infusion of 100 ml coix seed triglyceride was carried out during the performance of TACE, and postoperative intravenous drip of coix seed triglyceride (200 ml/d) was used for 5 days. The peripheral blood samples were collected one week before and one month after the treatment to detect the changes of AFP and T lymphocyte subsets (CD3﹢, CD4﹢, CD8﹢,CD4﹢/CD8﹢ and Treg) levels. One month after the treatment, enhanced CT, MRI or PET-CT was performed to evaluate the necrosis degree of the tumor. Results After the treatment, AFP levels was decreased in both groups, when compared the preoperative data the differences were statistically significant (P<0.01); the tumor necrosis rate of the study group was (57.7 ±8.2)%, which was slightly higher than (57.7±8.2)% of the control group, however, the difference was not statistically significant (P>0.05). In the study group, the percentage of Treg cells decreased from preoperative (8.27±6.65)%to postoperative (4.22± 1.59)%, the difference was statistically significant (P<0.01). The percentages of CD3﹢ and CD4﹢ and the ratio of CD4﹢/CD8﹢ increased from preoperative (55.78 ±13.66)%, (43.98 ±14.00)% and 1.22 ±0.64 to postoperative (62.29±10.78)%(P<0.01),(51.82±16.32)% (P<0.05) and 1.54±0.80 (P<0.05) respectively, while the percentage of CD8﹢decreased from preoperative (45.71±12.94)%to postoperative (39.70±12.41)%(P<0.05). In the control group, no statistically significant differences in the above mentioned indexes existed between preoperative data and postoperative ones (P>0.05). Conclusion In treating advanced primary HCC, coix seed triglyceride combined with TACE can reduce the percentage of Treg cells, thus, influence the patient’s cellular immune status and possibly decrease the recurrence rate of HCC after TACE therapy.