Animals ; Biomarkers, Tumor ; analysis ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Neoplasm Proteins ; secretion ; Neoplasms ; etiology ; pathology ; secretion ; Proteome ; secretion ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

OBJECTIVEThis study aims to investigate the dental caries status of 12-year-old children (born in 1998) in Sichuan province in 2010 and 2011, monitor the tendency of dental caries, and update available information and scientific basis for oral health care program.

METHODSThe oral health of 733 twelve-year-old children was surveyed for two consecutive years according to the methods proposed by the World Health Organization. The children were selected using multi-stage, stratified, randomized sampling. The collected data were analyzed using SPSS 17.0 software.

RESULTSCaries prevalence, DMFT, and DMFS in 12-year-old children were 34.8%, 0.93, and 1.09, respectively, and increased to 44.3%, 1.08, and 1.40 in the following year. The eruption of permanent teeth among females was higher than that of males (P < 0.05). The significant caries index was 2.54 in 2010 and 2.90 in 2011. F/D+F were 4.2% and 6.5% separately. Annual incidence of dental caries was 9.6%.

CONCLUSIONCaries prevalence in the permanent teeth of 12-year-olds is high and worsens as the children age. Despite the high incidence of dental caries, the filling rate is low, hence the need for immediate preventive measures.

Child ; China ; epidemiology ; DMF Index ; Dental Caries ; epidemiology ; Female ; Humans ; Male ; Oral Health ; Prevalence ; Prospective Studies ; Tooth Eruption

Objective To investigate the efficacy of microwave ablation for big liver cancer by using laparoscopy.MethodsFrom August 2000 to October 2006,28 patients with large big liver cancer(over 5 cm in diameter)was treated by microwave ablation under the guidance of a laparoscope.On each tumor nodule,3 to 5 electrode insertions was applied.And for each lesion,the treatment was performed for 1 to 3 times.Results The complete ablation rate of this series was 78.6%(22/28),and the rate of local recurrence was 35.7%(10/28).1-,3-,and 5-year survival rates were 75.0%(21/28),57.1%(12/21),and 36.4%(4/11),respectively.Conclusions Laparoscopic microwave ablation is safe and effective for liver cancer.The procedure can increased the efficacy of the microwave by enlarging the extent of the ablation.

Objective To improve the diagnosis and treatment of pulmonary lymphangiomyomatosis (PLAM). Methods The clinical data of 1 PLAM case in our hospital was analysed, along with a review of the related literatures.Results PLAM was a rare diffuse interstitial plumonary disease of unknown cause, which was oiraracterized by recurrent spontaneous pneumothorax shortness of breath (dyspnea) with physical activity, haemopthsis and chylothorax. Radiological appearances were extensive bilateral linear pattern, and honeycomb pattern in the end. High resolution CT scan (HRCT) showed multiple thin walled cysts distributed evenly throughout the bilateral lung fields with normal intervening lung parenchyma. Pathological characters were abnormal, pervasice multiplication of smooth muscle of cell in and around the small airway, lymphatic and small blood vessels. Immunohistochemical test showed HMB45(+).Conclusion It should be considered the probability of PLAM when women of childbearing ages have some clinical manifestations as follows, the increasing dyspnoea that can not be relieved similar to emphysema, high- resolution CT scan (HRCT) shows multiple thin walled cysts distributed evenly throughout the bilateral lung fields with normal intervening lung parenchyma, and recurrent pneumothorax or chylothorax. The optimal confirm diagnostic method is lung tissue biopsy.

Fibulin-1 is a widely distributed,versatile secretory calcium-binding glycoprotein which plays an important role on the formation of the structure of the extracellular matrix,the regulation of intercellular signaling and the inhibition of cell motility.Recent studies suggested a tumor-suppressive role for Fibulin-1.Here,this review focuses on the expression,function and mechanism of Fibulin-1 genes in tumor.

Objectives To investigate the effects of matrine on the proliferation and apoptosis of SK-NEP-1 cells in vitro, and its possible mechanism. Methods Trials were divided into following groups:control group, 0.5, 1.0 and 1.5mg/ml of ma-trine intervention groups. The inhibition rate of SK-NEP-1 cells treated with different concentration of Matrine was detected by MTT colorimetric assay. Apoptosis rate was detected by flow cytometry (FCM). RT-PCR analysis was employed to measure the PDCD4 mRNA expression. Results Matrine (final concentrations=0.5, 1.0 and 1.5mg/ml) could induce apoptosis and inhibit the growth of SK-NEP-1 cells. Compared with the controls without matrine treatment (8.81±3.71)%, the inhibition rates of SK-NEP-1 cells were (20.79 ± 6.20)%, (31.25 ± 5.07)%, and (51.15 ± 12.70)%, respectively;the apoptotic rates of SK-NEP-1 cells treated with different concentration of matrine were (13.67±0.78)%,(17.43±1.65)%and (20.80±1.54)%, respectively. Significant difference in the inhibition and apoptotic rates of SK-NEP-1 cells between each drug group and control group was observed(P<0.05), and the inhibition and apoptotic rates of SK-NEP-1 cells increased gradually with increased matrine concentration, thus exhibiting a dose-dependent effect(P<0.05). To the expression of CXCR4 mRNA,the grey levels of SK-NEP-1 cells treated with matrine intervention group (final concentrations=0.5, 1.0 and 1.5 mg/ml) were (0.720 ± 0.058), (0.540 ± 0.095) and (0.307 ± 0.050), respectively. The mRNA expression of CXCR4 was seen in SK-NEP-1 cells. Compared with control group, the expres-sion of CXCR4 mRNA was decreased significantly in matrine intervention group (P<0.01).There were significant difference in CXCR4 mRNA level among the SK-NEP-1 cells treated with 0.5,1.0,1.5mg/mL of matrine (P<0.01). Conclusions Matrine could induce apoptosis and inhibit the growth of SK-NEP-1 cells in a dose-dependent way which may be associated with the down-regulated CXCR4 expression in SK-NEP-1 cells.

Objective To investigate the expression of proliferating cell nuclear antigen(PCNA),vascular endothelial growth factor(VEGF) and microvessel density(MVD) in squamous cell carcinoma;and to explore the relationship between their expression and tumor biological behavior.Methods The expressions of PCNA,VEGF and MVD were analyzed by immunohistochemical method in 71 cases of esophageal squamous cell carcinoma.Results The intensity positive rate of PCNA indicated close relationship between cell differentiation and lymph node metastasis(P

The in-depth study on the law of drug pair compatibility and its mechanism has important scientific connotation for the clinical prescriptions and the improvement of curative effect. In recent years, many prevention and treatment of cerebral ischemic injury found that the rational use of Chinese materia medica pairs by optimizing the combination of Chinese medicine compound played a multi-target, multi-level role in the ischemic brain tissue of the neurovascular units, multi-channel regulation of the relevant signal pathways, which can significantly reduce the damage of ischemic penumbra brain tissue, relieve the inflammatory cascade and reperfusion injury caused by cerebral ischemia injury, promptly restore the blood flow in the brain and effectively protect the neurons, achieve “re-flow” and “brain protect” similar effects, and further promote the repair of nerve function.

Objective To evaluate the prognostic value of RAS association domain family 1A gene (RASSF1A) methylation in patients after hepatocellular carcinoma (HCC) hepatectomy.Methods A total of 260 patients with HCC who underwent hepatectomy were enrolled.HCC tissues and tumor adjacent tissues which were 2 cm away from the tumor edge of the patients were obtained.The clinicopathological data of patients were collected.The methylation of RASSF1A in HCC tissues and corresponding tumor adjacent tissues was determined by methylation specific polymerase chain reaction (PCR).The correlation between the expression rate of RASSF1A methylation and clinicopathological characteristics was analyzed by chi-square test.Log-rank test was performed to analyze the relation between RASSF1A methylation and overall survival rate.Univariate and multivariate Cox statistical techniques were used to identify the influence factors in the prognosis of HCC.Results Among 260 HCC tissues and corresponding tumor adjacent tissues,RASSF1A promoter hypermethylation was detected in 214 HCC tissues (82.3 ％) and 101 corresponding tumor adjacent tissues (38.8％),and the difference was statistically significant (x2 =102.824,P ＜ 0.01).There was no correlation between RASSF1A methylation and age,gender,liver cirrhosis,α-fetoprotein level,maximum diameter of tumor,Barcelona Clinic Liver Cancer (BCLC) stage,hepatitis B virus (HBV) infection,smoking and alcohol drinking (all P＞0.05).The 5-year overall survival rate of patients with negative RASSF1A methylation was 93％,while that of patients with positive RASSF1A methylation was 51 ％,and the difference in overall survival rate between the two groups was statistically significant (x2 =26.556,P ＜ 0.01).Univariate and multivariate Cox regression analysis indicated that liver cirrhosis,BCLC stage and RASSF1A methylation were the main influence factors in the death of patients with HCC after surgery (Wald=16.767,8.791,16.286; all P＜0.01).Conclusion RASSF1A methylation is not only one of the predictive factors of survival rate in patients with HCC after hepatectomy,but also an independent prognostic factor of HCC.

The fluorescence of Rhodamune B could be quenched by the manner of photo-unduced electron transfer wuth Cu2-x Se nanopartucles as the energy receptor and Rhodamune B as the energy donor. However, L-cysteune was capable of recoverung the fluorescence of Rhodamune B, and the fluorescence untensuty was proportuonal to the concentratuons of L-cysteune. Based on that, a novel method for detectung L-cysteune was establushed. After muxung L-cysteune and Rhodamune B pretreated by Cu2-x Se nanopartucles at pH 4. 6 and 30℃ for 2 mun, a lunear relatuonshup was obtauned between the fluorescence untensuty of Rhodamune B at 575 nm and the concentratuons of L-cysteune un the range of 2. 5 ×10-7-1. 1 ×10-6 mol/L. Thus method was used un the determunatuon of L-cysteune wuth a detectuon lumut (3σ/k) of 5. 5×10-8 mol/L. The common amuno acuds presented luttle unterference for the L-cysteune detectuon.