Henoch-Sch(o)nlein purpura(HSP) is one of the most common vasculitides in children with many manifestations including skin purpura,arthritis,abdominal pain and renal involvement.The renal involvement(Henoch-Sch(o)nlein purpura nephritis,HSPN) is the principal cause of morbidity and major risk factor for prognosis in HSP.The paper reviewed the updated treatment strategy for HSPN.Accordingly,steroids,cyclophosphamide,mycophenolate mofetil,mizoribine,cyclosporine,triptolide,angiotensin converting enzyme inhibitors (ACEI) / angiotensin receptor blockers(ARB),plasma apheresis and tonsillectomy are possible indicator for the further therapy of HSPN.Furthermore,prospective randomized controlled treatment studies on children with severe HSPN would be needed.

[Objective] This article mainly discussed the potential of myocardial cellregeneration and the possible mechanisms involved. [Method] To study the mechanism of cardiac regeneration ability HSCs, this review studyed the relationship of the differentiation of cardiac stem cells, hematopoietic stem cells, myocardial cells and myocardial regeneration and the possible mechanisms involved. [Result] Self-renewing, differentiation both in vitro and in vivo of the c-kit positive CSCs had been seen. No matter in physiological condition or after damage, the c-kit positive CSCs had clonality and the potential of multi-directional differentiation. Hematopoietic stem cells can generate cardiomyocytes and coronary blood vessels for human celltherapy. The ability of dedifferentiation cells to maintain and improve the ventricular function of a damaged heart is very limited. [Conclusion] CSCs and HSCs play an important role in reversing chronic ischemic and non-ischemic heart failure.

Objective To investigate the clinical effect of three capsule combined with Function injection combined with functional exercise in the treatment of knee osteoarthritis.Methods120 patients with knee osteoarthritis were randomly divided into two groups, the study group took orally hydrochloride capsule combined with joints injection combined with functional exercise therapy, and the control group only oral glucose capsules.two groups of patients after a period of treatment.ResultsIn the VAS score, lequesne index and serum inflammatory molecular level of the two groups were significantly better than the control group (P<0.05).ConclusionWhich the clinical study on treating of the knee osteoarthritis by using glucosamine capsule and injecting sodium hyaluronate combined with appropriate functional exercise has a significant effect on knee osteoarthritis, and more favorable in improving the quality of life of patients.

The early treatment principles of spinal cord injury (SCI) included early drug using, early surgery and early rehabilitation.The literatures on how to use drug early and how to control the time for SCI before surgery were reviewed.

Objective To investigated the neuroprotective effect of PTEN inhibitor BPV on cerebral ischemia-reperfusion injury in rats and its mechanism. Methods Male Sprague-Dawley rats were used to induce a reperfusion model of middle cerebral artery occlusion for 1 h. During the reperfusion, the BPV solution (0. 2 mg/kg daily) or the equal volume of saline was injected intraperitonealy immediately. The neurological deficit scores were conducted at day 1, 3,5, and 7 after ischemia-reperfusion. At day 4, triphenyl tetrazolium chloride staining was used to assess cerebral infarction volume. Enzyme-linked immunosorbent assay was used to detect the levels of interleukin 10 (IL-10) and tumor necrosis factor α(TNF-α) in cortical ischemic border zones. Real-time quantitative polymerase chain reaction was used to detect the expression level of PTEN mRNA. Western blotting was used to detect the expression levels of PI3K, Akt, and p-GSK-3β. At day 7, Bielschowsky silver staining was used to detect the axonal distribution in the ischemic border zone of the striatum. Immunohistochemical staining was used to detect the expression of myelin basic protein (MBP). Results At day 4 after ischemia-reperfusion, the infarct volume (32. 27% ± 1. 71% vs. 45. 49% ± 2. 12% ; P < 0. 001), TNF-α concentration in the cortical ischemic border zones (134. 17 ± 10. 38 pg/ml vs. 264. 17 ± 24. 84 pg/ml; P < ), and PTEN mRNA level (1. 19 ± 0. 08 vs. 2. 50 ± 0. 06; P < 0. 001) in the rats of the BPV group were al significantly lower than those of the normal saline group. The IL-10 concentration (186. 83 ± 10. 83 pg/ml vs. 147. 83 ± 11. 62 pg/ml; P < 0. 001), and the expression levels of PI3K (0. 43 ± 0. 08 vs. 0. 26 ± 0. 06; P = 0. 004), Akt (0. 52 ± 0. 05 vs. 0. 40 ± 0. 04;P = 0. 001), and p-GSK-3β (0. 75 ± 0. 08 vs. 0. 38 ± 0. 06; P < 0. 001) were al significantly higher than those of the normal saline group. At day 7 after ischemia-reperfusion, the neurological deficit score (4. 83 ± 0. 41 vs. 6. 33 ± 0. 52; P < 0. 001) in the rats of the BPV group was significantly lower than that of the normal saline group. The axon densities in the ischemic border zones (35. 51% ± 2. 45% vs. 25. 31% ± 2. 79% ; P < 0. 001) and the expression level of MBP (32. 56% ± 3. 46% vs. 27. 81% ± 4. 18% ; P = 0. 037) were significantly higher than those of the normal saline group. Conclusions BPV has neuroprotective effect for cerebral ischemia-reperfusion injury in rats. Its mechanism may be associated with the up-regulation of PTEN downstream proteins PI3K, Akt and p-GSK-3β expression to regulate inflammatory mediators and reduce the inflammatory response.

Objective:To improve the quality standard for Anweiyang capsules. Methods: TLC was used to identify Glycyrrhiza inflata Bat. Licochalcone A was determined by RP-HPLC. Using a C18 Column, the mobile phase consisted of acetonitrile-0. 1% phos-phoric acid(40:60), and the detection wavelength was at 376 nm. Results:The herb could be identified by TLC. For licochalcone A, the linear range was from 25. 563 to 1 533. 798 ng, and the average recovery was 99. 8%(n=9). Conclusion:The method is simple and accurate, which can be used to improve and control the quality of Anweiyang capsules.

Objective To evaluate the correlation between high-sensitivity C-reactive protein(hsCRP)and neutrophil alkaline phosphataee(NAP).Methods 74 patients with different hsCRP levels and 20 healthy subjects were selected.hsCRP and NAP were detected in every person,then the t test and correlation analysis were conducted.Results The NAP integral value of high-value hsCRP group and mid-value were significantly higher than the low-value hsCRP group(P ＜ 0.01),and the NAP group average positive rate of the high,median hsCRP values were 81.8％and 82.3％,which were higher than low-value of hsCRP group(53.6％).Compared the correlation between each group of hsCRP value and integral value of NAP,the hsCRP of high-value hsCRP group has a good correlation with NAP integral value(P ＜ 0.01),while the hsCRP of mid-value of hsCRP group and NAP integral is less relevant(P ＞0.05).Conclusion hsCRP as an acute reactive protein exists a good correlation with NAP,when hsCRP ＞ 50mg/L,it can partially replace NAP.

0.05). VIP, however, could inhibit the Con A-induced proliferation of T-cells from control subjects more significantly than that from asthmatics (P0.05). The cAMP level in T-cells, however, increased more significantly in the control group than that in the asthmatic group after the treatment of VIP or NaF (P0.05). CONCLUSION: Inhibition effect of VIP on Con A-induced proliferation of T-cells was less in asthmatics than in control subjects, which may be related to insufficiency of Gs ? coupled VIP receptor on T-lymphocytes in asthmatics.

[Objective]To evaluate short term clinic results of prosthetic disc nucleus(PDN)replacement for the treatment of lumbar disc herniation.[Method]Twenty cases of lumbar disc herniation(including one case of recurrent lumbar disc herniation)were treated with PDN from June 2003 to November 2003,including 13 males and 7 females with average age of 40.5 years.All cases were implanted with a single PDN,in which 7 cases with PR725,8 cases with PW725,and 5 cases with PDN-SOLO-7 respectively.[Result]After surgery,one patient accepted the revision operation to remove the PDN because of device migration,the others experienced pain relief.The nineteen cases were followed-up for 23～29 months(mean 26.4 months).Compared with preoperative height of intervertebral disc,it gained increase of 17.2%(P

Objective To investigate clinical features and anterior surgical results of cervical myelopathy in patients more than 70 years of age. Methods Twenty aged patients with cervical myelopathy who underwent anterior decompression with bone graft surgery were reviewed. Neurological function was assessed using a scoring system proposed by the Japanese Orthopaedic Association (JOA score). The clinical results and complications were compared with the control group which included 31 patients with multisegmental cervical myelopathy and less than 69 years old and underwent the same operation. Results The preoperative mean JOA score was 9.3 (ranged from 3 to 14) and the JOA score at latest follow-up averaged 13.4 (ranged from 8 to 17) in the aged patient group. 68 percent patients had achieved an excellent or good result and the recovery rate was 58%. 71 percent patients had achieved an excellent or good result and the recovery rate was 67% in the control group. No statistical difference was found in the excellent and good result rate or the recovery rate of JOA score between the aged group and the control group (?字2=0.04, P=0.85;t=1.12, P=0.138). The incidence of surgical related complications in the aged group was 35% (7 cases), which was considerably higher than that in the control group (3 cases, 10%) but without statistical difference (?字2=3.47, P=0.06). In the preoperative flexion-extension stress lateral radiographs, the incidence of cervical instability was higher in the control group (8 cases, 26%) than that in the aged group(1 case, 5%). Conclusion 1) Multilevel lesion induced by overcompensation for cervical instability are the probable cause of myelopathy in aged patients. 2) Anterior decompression is beneficial to improve the neurological function and life style in the aged patients with cervical myelopathy, but associated with a high incidence of surgical complications.