Objective] The two-step massage treatment of tennis elbow and simple massage therapy clinical curative effect around the elbow. [Method] A total of 80 cases of external humeral epicondylitis were randomly divided into the treatment group(42 cases) and control group(38 cases). The treatment group were treated with the combination of the neck and elbows. The control group was treated only with simple elbow massage. [Results] The treatment group cure rate was 71.4%, the total efficiency of 92.9%;control group, the cure rate was 34.2%, the total effective rate was 71%. The comparison between the 2 groups, the cure rate and total effective rate differences were significant( P＜0.01, P＜0.05). [Conclusion]Two-step massage has better cure effect on external humeral cpicondylitis.

Objective The purpose of this study was to develop a new cultural method, so that the rat bone marrow stromal cells (MSCs) can well differentiate into osteoblasts in vitro and the cultured osteoblasts can be identified. Methods MSCs of rats were isolated and cultured, then the cultured cells were identified with inverted microscopy and undergone an HE stain for observing the morphology of living cells, and the cells were histochemically stained for examining the activity of cellular alkaline phosphatase (ALP), as well as were radio-immunologically analyzed for detecting the secretion of osteocalcin. Results The MSCs were typical fibroblast-like and possess a strang proliferative capability after cultured in vitro.The cellular ALP activity and osteocalcin secretion achieved the peak value from the fortieth day and maintained the status until the sixtieth day after cultured in vitro.Conclusion The data demonstrated that the new developed culture method has the advantages of short time, less risk of contamination and higher efficiency, and conducive to MSC's differentiating and proliferating into osteoblasts that do function well.

The effects of carbamates and soman on the metabolism of nicotinic acetylcholine receptors (N-AChR) in cultured skeletal muscle cells were studied by 125I-a-bungarotoxin (125I-a-BTX), a specific marker. It was indicated that N~AChR degradation process was inhibited and the incorperation rate of IshAChR and the number of N~AChR in the surface of the cell membrane were increased 4h after prertreatment of high concentrations of carbamates. The number of N-AChR was also increased after pre-treatment of low concentrations of soman, whose effects on N-AChR metabolism were similar to those of high concentrations of carbamates. The incorperation rate of N-AChR was observed by inhibiting the protein synthesis with puromysin, suggesting that soman may increase the number of N-AChR by increasing the incorperation of N-AChR instead of increasing the synthesis of N-AChR.

Rhizome anemarrhena,sweet and bitter to taste,cold,in the lung,stomach and kidney meridian,has the function clearing away heat and purging pathogenic fire,nourishing Yin and moistening dryness.Zhang Zhong Jin in "Essential Prescriptions of the Golden Chamber "with a different combination of herbs and Rhizome anemarrhenae,reflects the multiple efficacies of TCM and compatibility.

OBJECTIVE:To study the effect and its mechanism of dexmedetomidine(Dex)on inflammatory response in septic mice. METHODS:Mice were randomly divided into normal control group,model group,miR-146a inhibitor (50 mg/kg)+Dex (50 μg/kg)group,Dex low-dose,medium-dose,high-dose groups(10,30,50 μg/kg),10 in each group. Except for normal con-trol group,other groups were intraperitoneally injected lipopolysaccharide to induce septic models,intraperitoneally injected rele-vant medicines after 0.5 h. After drug intervention for 6 h,miR-146a expression,IRAK1 and TRAF6 mRNA expressions in periph-eral blood mononuclear cells in each group were detected by real-time fluorescence quantitative polymerase chain reaction method. IRAK1,TRAF6 protein expressions in peripheral blood mononuclear cells in each group were detected by Western blot method. TNF-α,IL-6 levels in serum were detected by enzyme-linked immunosorbent method. RESULTS:Compared with normal control group,miR-146a expression,TNF-α and IL-6 levels,IRAK1,TRAF6 mRNA and protein expressions in peripheral blood mononu-clear cells in model group were increased(P<0.01). Compared with model group,miR-146a expression in peripheral blood mono-nuclear cells in Dex medium-dose,high-dose groups were increased;TNF-α and IL-6 levels,IRAK1,TRAF6 protein expressions were decreased (P<0.05 or P<0.01);while IRAK1,TRAF6 mRNA expressions changed less obviously (P>0.05). Compared with Dex high-dose group,miR-146a expression,in peripheral blood mononuclear cells in miR-146a inhibitor+Dex group was de-creased;TNF-α and IL-6 levels,IRAK1,TRAF6 protein expressions were increased (P<0.05 or P<0.01);while IRAK1, TRAF6 mRNA expressions changed less obviously(P>0.05). CONCLUSIONS:Dex can inhibit the inflammatory response in sep-tic mice. The mechanism may associate with inducing miR-146a expression and inhibiting the 2 important adaptor proteins IRAK1, TRAF6 expressions in Toll-like receptor 4/nuclear factor-κB pathway.

Objective To study MRI characteristics and value in the diagnosis of nasopharyngeal carcinoma with skull base erosion. Methods 86 cases of nasopharygeal carcinoma with positive nasopharyngeal pathology and symptoms of skull base erosion were selected. Axial T1WI, T2WI and coronal, sagital T1WI were performed. 47 cases had axial, coronal and sagital T1WI examination post Gd-DTPA injection. Results Areas of skull base involvement were as follow: 56 cases with pterygoid process, 54 with clivus, 28 with foramen ovale (including sphenoid wing), 27 with petrous apex, 1 with occiptal bone. 71 cases had more than one area involvement. There are four patterns in our group: ①Defect in the signal avoid cortex of skull base; ②High signal intensity of marrow was replaced by tumor; ③Contrast enhancement in abnormal bone; ④String like enhancement passing nerve foramen in skull base. 44 cases included intracranial abnormal. Conclusion MRI is the preferred technique in dementstrating skull base involvemetn of nasopharyngeal carcinoman.

There are so many patients with different liver diseases in our country that the diagnosis and treatment of liver diseases directly relate to the people's healthy level.The requirement of diagnosis and treatment to clinical laboratories can be summarized as: ( 1 ) sensitivity,which means shortening window period;(2) specificity,which means various subtypes and variant can be detected;( 3 )speed,which means finding out the pathogen as soon as possible;(4)accuracy,which means providing physicians with accurate test reports.Detecting techniques,for instances,chemiluminescence,polymerase chain reaction and gene mutational site detecting method,were applied to the diagnosis and treatment of liver disease,thus improving the degree of precision,accuracy,sensitivity,specificity and detection speed and satisfying the requirement of diagnosis and treatment maximatily.

Objective To observe the changes of serum interleukin and T lymphocyte subsets levels in patients with gallstones before and after treatment. Methods 72 patients with gallstones were treated as research subjects and 30 cases with normal physical examination as the control group,the serum interleukin and T lymphocyte subsets levels of observation group were detected before and after surgery. Results The preoperative serum IL-6, IL-8 and TNF-α levels of observation group were(61.5 ±4. 8) ng/L, (246. 2 ±15.3)ng/L and(1.79 ±0.72)mg/L respectively which were significantly higher than postoperative and control group,the difference was statistically significant (P ＜ 0. 05 ) , while there was no significant difference between postoperative and normal control group ( P ＞ 0.05); The preoperative CD3+ , CD4+ and CD8+ T cells ratio of observation group were (0. 53 ± 0. 06) % , (0. 28 ±0.05)% and(0.21±0.04)% respectively which were significantly higher than postoperative and control group,the difference was statistically significant(P ＜ 0.05) , while there was no significant difference between postoperative and normal control group(P＞0.05). Conclusion Patients with gallstone had obvious abnormal serum IL-6, IL-8, TNF-α,CD3+ ,CD4+ and CD8+ T cells levels,surgery could not only eliminate inflammation,but also help the recovery of immune function in patients.

Objective To investigate the effect of intravenous injection of tranexamic acid on reducing bleeding effect in invasive total hip replacement.Methods In our hospital from April 2015 to April 2016, 50 cases of total hip joint replacement patients as the object of study, patients were randomly divided into observation group and control group, 25 cases in each group.The control group were injected with 0.9% NaCl injection;observation group received intra-articular injection of tranexamic acid after suture, the transfusion volume of blood, complications, hemoglobin concentration, and rate of blood transfusion were compared between the two groups.Results The rate of blood transfusion in the control group was 52.0%, significantly higher than that of the observation group (24.0%), and the difference was statistically significant (χ2 =4.16, P<0.05).The drainage (260.1 ±78.6) mL and transfusion volume (230.8 ±60.1) mL in observation group were better than control group (370.04 ±56.2)mL and (380.2 ±40.6)mL,with statistical significance (P <0.05).The hemoglobin concentration in observation group was lower than control group(P <0.05).There were 2 cases of hematoma, 5 cases of paining in observation group and 3 cases of hematoma, 6 cases of paining in control group, with no statistical significance (χ2 =0.368, P>0.05).Conclusion Tranexamic acid is the main method for prevention of postoperative bleeding, but could not reduce postoperative complications.

Objective To investigate the effect of head extension angle to glottis exposure by using three-dimensional CT reconstruction sagittal plane images of upper airway.Methods There were 80 patients,aged 7-78 years old,who had been scanned by helical CT in the head and neck.Reconstructed images were obtained by AW4.4 workstation and the following parameter was measured:angle α(A0 group),the angle created by the upper central incisor and root of epiglottis(angular vertex) and midpoint of glottis.Reconstructed images were printed according to the actual size of human body in a ratio of 1∶ 1,then the images were cut off from the upper central incisor to root of epiglottis by a section of arcs.Increasing the degree of angle α by clockwise to hypothetically increase the angle of head extension.The glottis was exposed by using a paper-made laryngoscope(Macintosh) model,and the success rate of glottis exposure was recorded when the degree of angle α was respectively increased 10°(A10 group),20°(A20 group),and 30°(A30 group).Results Compared with A0 group,the success rate of glottis exposure increased significantly in A10 group,A20 group and A30 group.The success rate of glottis exposure in A20 group and A30 group was significantly higher than A10 group.Compared with A20 group,the success rate of glottis exposure obviously increased in A30 group.Conclusion Based on CT reconstruction images of upper airway,we can analog study the effect of head extension angle to glottis exposure.