Background and objective Long non-coding RNA (lncRNA) plays important regulatory roles in the development and invasion of various cancers. hTe aim of current study is to comprehensively identify microRNAs (miRNA) regulated by lncRNA MALAT1 via experimental and bioinformatics methods.Methods Antisense oligonucleotides (ASO) speciifcally targeting MALAT1 were designed and synthesized. Atfer knockdown of MALAT1 by ASO in A549 cells, miRNA expression changes were proifled by TqaMan Low Density Array (TLDA). Gene set enrichment analysis (GSEA) was used to search enriched miRNAs among differentially expressed genes atfer knockdown of MALAT1.ResultsAtfer effcient knock-down of MALAT1 by ASO, 153 miRNAs were differentially expressed, 131 up-regulated and 22 down-regulated. Among the 458 differentially expressed genes atfer MALAT1 silence, GSEA results revealed lots of enriched miRNAs. hTere were 28 over-lapped miRNAs between TLDA and GSEA results, suggesting these 28 miRNAs are regulated by MALAT1.Conclusion hTis study comprehensively identiifed MALAT1 regulated miRNAs, providing resources for further research.

Background and objective Glypican-5 (GPC5) is an important tumor suppressor, while little is known about the impact of GPC5 on proliferation ability and gene expression in lung adenocarcinoma cell lines. Here, we stably over-expressed GPC5 in A549 cells and investigated the impact of cell proliferation ability and gene expression.MethodsA549 cells that stably overexpressed GPC5 were constructed by lentivirus. Cell counter kit 8 (CCK8), colony formation, EdU assay were conducted to analyze cell proliferation ability, and transcriptome sequencing was utilized to investigate gene expression proifle. ResultsCCK8 assay showed that compared with empty vector, overexpression of GPC5 signiifcantly inhibited cell pro-liferation rate in A549 cells and the number of colony was also decreased (181±17vs 278±23). EdU assay also conifrmed the percentage of positive staining cells decreased atfer GPC5 overexpression. Transcriptome sequencing revealed that 2,108 genes were differentially expressed atfer GPC5 overexpression. Among these differentially expressed genes, 47 genes of the Gene Ontology item “positive regulation of cell proliferation” were downregulated.ConclusionOverexpression of GPC5 inhibited proliferation ability in lung adenocarcinoma A549 cells and genes with the function of “positive regulation of cell proliferation” were downregulated.

Background and objective MicroRNAs were important regulators of tumors and the expression of miRNA-221 was associated with malignant proliferation and invasion in many tumors. hTe aim of this study is to explore the expression of miRNA-221 in non-small cell lung cancer (NSCLC) tissues and its correlation with prognosis. Methods We ret-rospectively analyzed the clinical characteristics of 117 NSCLC patients who underwent surgery in our hospital from Novem-ber 2005 to January 2007. Real-time PCR was used to detect the expression of miRNA-221. Chi-square test was utilized to ana-lyze the relationship between miRNA-221 expression and clinicopathologic features. Survival curves were plotted by using the Kaplan-Meier method and compared by the Log-rank test. A Cox proportional hazard regression model was applied to examine the joint effect of covariants. A P-value less than 0.05 was evaluated as statistically signiifcant. Results hTe patients were di-vided into two groups according to the relatively expression of miRNA-221. No statistically signiifcance was observed between the expression of miRNA-221 and the clinicopathologic parameters, including gender (χ2=0.070, P=0.791), histology (χ2=0.414, P=0.520), p-TNM stage (χ2=0.068, P=0.794) and history of smoking (χ2=0.206, P=0.650). Kaplan-Meier analysis showed that high expression of miRNA-221 was closely associated with a shorter survival time (P<0.001). Finally, both univariate and multivariate analyses demonstrated that high miRNA-221 expression might be a poor prognostic marker of NSCLC patients. Conclusion Our results indicated that down-regulation of miRNA-221 was associated with poor prognosis of patients with NSCLC. MiRNA-221 may serve as a molecular prognosis marker for patients with NSCLC.

"Global cancer statistics 2022" based on the latest GLOBCAN data from the International Agency for Research on Cancer (IARC) was recently released, providing a systematic analysis of the incidence and mortality of 36 types of cancer across 185 countries worldwide. The international burden of cancer is expected to continue to increase over the next 30 years, posing a severe public health and social challenge for many countries, including China. This article offers a key point interpretation of the "Global cancer statistics 2022", focusing on the evolution of cancer epidemiology and future development trends. The aim is to broaden the international perspective on cancer prevention and treatment, with the hope of providing reference and guidance for cancer prevention and treatment efforts in our country.