Objective To investigate and analyze the health education requirement in migrant-worker patients and establish health education countermeasures that accord with migrant-worker pa-tients. Methods 600 hospitalization patients were chosen from January to December, 2007,among which 300 migrant-worker patients were set as the experimental group,300 native patients were selected as the control group.The contents and manners of health education requirement were compared between the two groups. Results In the aspect of contents of health education requirement,the highest require-ment was to understand treatment expense,which was significantly higher than that of the control group. The higher requirement included to understand illness condition and treatment protocols,prevention and treatment of common disease and infectious disease.In the aspect of manners of health education require-ment,the highest requirement was to communicate with medical workers,the lowest requirement was to communicate among patients. Conclusions Migrant-worker patients concern about treatment expense, nurses should not only conduct health education about disease-related knowledge, but also pay much atten-tion to informed consent about treatment expense in order to avoid unnecessary dispute.

OBJECTIVE:To investigate the curative effect and economics of prostaglandins drugs in the treatment of glauco-ma,and to provide reference for clinical medication. METHODS:In retrospective study,a total of 790 glaucoma patients were di-vided into latanoprost group(62 cases),travoprost group(356 cases)and bimatoprost group(372 cases)according to therapy regi-men. They were given relevant medicine. Total effective rate of 3 groups were calculated,and the cost-minimization method was used for pharmacoeconomics evaluation. RESULTS:The total effective rate of 3 groups were 87.10%,84.27%,76.08% respective-ly,without statistical significance(P>0.05). The cost of them were 208.00 yuan,225.00 yuan and 173.00 yuan,and that of bima-toprost group was the lowest. The results of sensitivity analysis was in line with that of cost-minimization analysis. CONCLU-SIONS:For glaucoma,bimatoprost is more economical than latanoprost and travoprost.

PBL is a new teaching method.Applying it to the teching of distribution of bacterias can fully display students'subjective initiative and have good teaching effect ant at the same time build the new model of PBL in medical education.

AIM　To study the activation of choline on M3-R in heart and observe the hemodynamic changes of rat and rabbit. METHODS　A cardiac catheter was inserted into the left ventricular cavity via the right carotid artery, then the HR, LVSP, LVEDP, and ±dp/dt were measured using a polygraph system. RESULTS　Choline was shown to decrease the hemodynamic assessments, such as HR, +dp/dt, LVSP and LVEDP. while the M3-R antagonist 4-DAMP (4-diphenylacetoxy-N-methylpiperidine-methiodide) showed little effect on these assessments. It was found to reverse the hemodynamic effects of choline. CONCLUSION M3 receptor agonist can produce negative inotropic and chronotropic effects on the heart of rat and rabbit.

Objective To study the preventive effect of volatile oil extracted from Foeniculum vulgare Mill. Seeds on the formation of postoperative intra-abdominal adhesion. Methods Thirty-eihgt SD rats were randomly divided into sham operation group (10), operation group (14) and volatile oil treated group (14):sham operation group was only operated by abdominal incision, the rest two groups were established animal model of abdominal adhesion by rubbing the procussus vermiformis of cecum with dry sterile gauze, clamping and scuffing abdominal wall. Half of rats were separately killed on day 7 and day 14 after surgery, respectively.The degree of adhesion was evaluated according to Phillips 5-scale grade and the feature of this model. Results The scores of intra-abdominal adhesion were significantly lower in the carbachol group than those in operation group both on 7 d and 14 d(P＜0.01 ). Conclusion Volatile oil extracted from Foeniculum vulgare Mill. Seeds may take a significant role in the prevention of postoperative abdominal adhesion in rats.

Objective To observe the effects of fennel essential oil and water extracts (distilled oil is not included) on gastrointestinal motility disorder caused by atropine in mice.Methods Kunming mice were randomly divided into blank control group, model group atropine, water extracts group, fennel essential oil group, mosapride group. Blank control group and model group atropine were orally administered with normal saline of 0.2 ml/10 g. Water extracts group was orally administered with Water extracts (75 mg/ml) of 0.2 ml/10 g. Fennel essential oil group was orally administered with Essential oil of 300 mg/kg. Mosapride group was orally administered with mosapride(15 mg/ml). Subjects were orally treated for 3 d. After fasting for 18 h, blank control group was intraperitoneally injected saline on the fourth day, and other groups were injected atropine sulfate injection to induce animal model of gastrointestinal motility disorder. Blue dextran(BD)2000 was used to observe the gastric emptying rate and rate of intestinal propulsion. Results Gastric emptying rates of fennel essential oil group, mosapride group, water extracts group and model group atropine were respectively(91.97±4.42)%, (90.26±5.81)%, (80.01±6.27)%,(72.88±9.13)%,and intestinal pushing rates were respectively(53.32±7.49)%,(53.02±9.13)%,(44.16±7.68)%,(37.52±6.19)%.Fennel essential oil, mosapride and water extracts enhanced the gastric emptying and intestinal propulsion in gastrointestinal motility disorder animal caused by atropine(P values were 0.004、0.001、0.004、0.003、0.025、0.015),where Fennel essential oil and mosapride were superior to the water,extracts(P values were 0.000、0.002、0.001、0.001).Conclusion Fennel extracts may promote gastrointestinal movement of atropine-induced gastrointestinal motility disorder in mice and fennel essential oil is the main active ingredient.

The age-related changes of serum lipid peroxide (LPO), RBC supero-xide dismutase activity (SOD) and glutathione peroxidase activity (GSH-Px), Cu, Zn and Se of blood were observed in 217 normal male persons aged 6~82. It was found that LPO increased significantly with age and SOD, GSH-Px decreased significantly in the elderly (60-70yrs). Blood Zn, Cu and Zn/Cu were highest in the childhood and lowest in the elderly. No significant change of Se with age was observed.The stepwise regression analysis showed that the factors influencing aging mainly were LPO and GSH-Px. It seems that LPO can be used as an indicator of aging.LPO was positively but GSH-Px, SOD, Zn and Cu negatively correlated with aging.

OBJECTIVE: To explore the risk factors of the newborns who failed initial hearing screening by analysing the distortion production otoacoustic emission (DPOAE) results of 1021 newborns with potential risk factors of hearing loss. METHOD: All newborns, who were born in obstetrical department and admitted in the neonatal department of the Nanfang Hospital during June 2009 to January 2012 and underwent initial hearing screening, were included in this study. Their clinical data and DPOAE results were analyzed retrospectively in order to identify the risk factors for failure of initial hearing screening in infants; cases who failed the DPOAE test were followed up by telephone interviews. RESULT: (1) One hundred and thirty-seven cases (13.42%) of the 1021 newborns did not pass the hearing screening. 51 cases (5.00%) did not pass the test in both ears. Meanwhile, left ear in 47 cases (4.60%) and right ear in another 39 cases (3.82%) failed the test respectively. (2) Univariate analysis showed that 14 factors had significant influence on the hearing screening results, such as birth weight, small for gestational age, multiple pregnancy, gestational age, delivery mode, oligohydramnion, oxytocin, blood sugar level of newborn, Apgar scores at 1 min, exposed prenatally to glucocorticoid, maxillofacial deformity, hypoxic-ischemic encephalopathy, neonatal respiratory distress syndrome and neonatal asphyxia (P < 0.01). (3) Multivariate Logistic regression analysis suggested that birthweight less than 1500 g, multiple pregnancy, Apgar scores of 0-4 at 1 min, exposed prenatally to glucocorticoid and maxillofacial deformity were risk factors for failure of initial hearing screening (OR were 3.132, 1.808, 2.615, 1.827 and 12.174 respectively; 95% CI were 1.466-6.691, 1.120-2.917, 1.317-5.336, 1.130-2.953 and 1.986-74.632 respectively). (4) Results of telephone interviews revealed that Apgar scores of 0-4 at 1 min would be a risk factor of language development. CONCLUSION Birthweight less than 1500 g, multiple pregnancy, Apgar scores of 0-4 at 1 min, exposed prenatally to glucocorticoid and maxillofacial deformity are risk factors of failure of initial hearing screening among newborns with potential hearing loss. Monitoring of the hearing condition of the infants at risk should be strengthened.
Deafness ; diagnosis ; Female ; Hearing Disorders ; diagnosis ; Hearing Tests ; Humans ; Infant, Newborn ; Neonatal Screening ; Otoacoustic Emissions, Spontaneous ; Pregnancy ; Risk Factors

Cellular prion protein (PrP(C)) is ubiquitously expressed in the cytomembrane of a considerable number of eukaryotic cells. Although several studies have investigated the functions of PrP(C) in cell proliferation, cell apoptosis, and tumorigenesis of mammals, the correlated functions of chicken PrP(C) (chPrP(C)) remain unknown. In this study, stable chPrP(C)-downregulated Marek's disease (MD) virus-transformed avian T cells (MSB1-SiRNA-3) were established by introducing short interfering RNA (SiRNA) targeting chicken prion protein genes. We found that downregulation of chPrP(C) inhibits proliferation, invasion, and migration, and induces G1 cell cycle phase arrest and apoptosis of MSB1-SiRNA-3 cells compared with Marek's disease virus-transformed avian T cells (MSB1) and negative control cells. To the best of our knowledge, the present study provides the first evidence supporting the positive correlation between the expression level of chPrP(C) and the proliferation, migration, and invasion ability of MSB1 cells, but appears to protect MSB1 cells from apoptosis, which suggests it functions in the formation and development of MD tumors. This evidence may contribute to future research into the specific molecular mechanisms of chPrP(C) in the formation and development of MD tumors.
Animals ; Apoptosis* ; Carcinogenesis ; Cell Cycle ; Cell Proliferation ; Chickens ; Down-Regulation* ; Eukaryotic Cells ; Mammals ; Marek Disease* ; RNA, Small Interfering ; T-Lymphocytes*

This study was conducted to explore whether cholecystokinin-B/gastrin receptor (CCKBRwt) gene and its alternative splicing variant (CCKBRi4sv) are expressed in human gastric carcinomas cell line and tissues, and to find out their relationship with the development and progression of human gastric carcinoma. The mRNA expression levels of CCKBRwt and CCKBRi4sv were detected in 30 human gastric carcinomas and normal tissues adjacent to cancer, 10 gastritis specimens, 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells. The results revealed that the transcripts of CCKBRwt and CCKBRi4sv were observed in all of the human gastric specimens tested, but only CCKBRwt was expressed in gastric cancer cell line SGC-7901 cells. The expression levels of the two receptors were not correlated with the differentiation and metastases of gastric cancers. From the results, we infer that human gastric tissues simultaneously express CCKBRwt and CCKBRi4sv, and CCKBRi4sv may have unknown physiological functions in gastric epithelial cells.
Base Sequence ; Epithelial Cells ; metabolism ; Gastric Mucosa ; metabolism ; Gastrins ; biosynthesis ; genetics ; Gastritis ; genetics ; metabolism ; Humans ; Molecular Sequence Data ; RNA, Messenger ; biosynthesis ; genetics ; Receptor, Cholecystokinin B ; biosynthesis ; genetics ; Stomach ; metabolism ; Stomach Neoplasms ; genetics ; metabolism ; Tumor Cells, Cultured