Objective To explore the herpes virus Ⅱ(HSV-2) infection and Ki-67 expression for the cervical carcinoma and cervical intraepithelial neoplasia(CN).Methods The polymerase chain reaction(PCR) and immunohistochemistry were applied to detect the HSV-2 and Ki-67 expressions in cancerous tissues of uterine cervix in 84 cases (67 scaly cell carcinoma,3 scaly adenocarcinoma,and 14 adenocarcinoma cases),CN in 35 cases,and normal cervical tissues in 13 cases.Results (1)The positive rote of HSV-2 is 40.2% (53/132),and the rate in cervical cancerous tissues,CN and normal cervical tissues were 48.8% (41/48) ,28.5% (10/35) and 15.4% (2/13) respectively.HSV-2 DNA expressions in cancerous tissue and CN were much higher than that of normal cervical tissues (P<0.01).(2)The Ki-67 positive cells were progressive increasing in the normal cervical tissues,CN and cervical cancerous tissues,thus showing that there were relations between the Ki-67 expression degree and tissue types.The HSV-2 and Ki-67 expressions were remarkably connected(P < 0.01).Conclusion There are HSV-2 infection ,close related between HSV-2 infection and Ki-67 expression in the tissues of cervical carcinoma and CN.And the cooperation of them may play an important role in the development of cervical carcinoma.

Objective To analysis of the causes, operative methods and the clinical results in patients with dislocated intraocular lens(IOL). Methods 11 cases (11 eyes) underwent surgical management of dislocated IOL were analyzed retrospectively. Results In the all cases trauma was the main cause; Surgical techniques included IOL exchange in 3 eyes and IOL removal in 1eye,removal combined with re-implantation in 5 eyes, simple cut the after cataract in 2 eyes, the final best-corrected visual acuity was 0.6~0.8 in 3 patients (27.27%), 0.4～0.5 in 4 patients (45.45%) ,0.2~0.3 in 2 patients (18.18%) and

Objective To observe the effect of early intervention on motor and mental development in premature infants. Methods 108 cases of hospital-born premature infants were divided into early intervention group (n=57) and control group (n=51). All the cases accepted conventional treatment and health-care, and the intervention group accepted the early intervention in addition. They were assessed with psychomotor development index (PDI) and mental development index (MDI) of Bayley Scales of Infant Development when they were 1 or 2 years old. The incidence of cerebral palsy when they were 2 years old were recorded. Results 2 cases (1 for each) lost during the follow-up.The PDI and MDI were more in the intervention group than in the control group (P<0.05), both as the 1 and 2 years old. The incidence of cerebral palsy was less in the intervention group than in the control group (P<0.05). Conclusion The early intervention can promote mental and motor development in premature infants, and reduce the incidence of cerebral palsy later.

Objective To know the effect of pre-hospital emergency care for patients with acute cranio-cerebral injury. Methods Selected 312 patients with acute cranio-cerebral injury from Jan of 2005 to Jan of 2009 as the emergency group, selected 285 patients with acute cranio-cerebral injury from Jan of 2000 to Dec of 2004 as the control group. Retrospective analized the clinical condition between the two groups to know the effect of pre-hosptial emergency cares. Results There was significant difference of dead rate betweent the two groups, the prognosis of the two groups was also different. Conclusions Effective pre-hospital emergency care is very important for patients with acute eranio-cerebral injury, which can avoid certain complications and reduce dead rate.

PurposeWith the extensive use of percutaneous radiofrequency ablation (RFA) for the treatment of hepatic carcinoma (HC), the study of MRI findings and its clinical signiifcance after RFA of HC have important value and can improve the complete ablation rate.Materials and MethodsA retrospective analysis of post-procedure MRI ifndings of 79 patients (114 lesions) with HC were performed, the size of the lesion, the signal changes and enhancement condition were observed at the ifrst, fourth and seventh month after RFA; the two different ifndings of high signal ring on MRI T1WI and local recurrence rate were analyzed.ResultsOne month after RFA, peripheral region of RFA lesion showed high signal on T1WI, and slightly lower signal on T2WI, the size of lesions was slightly larger than pre-procedure, enhancement scan showed the thin homogeneous ring enhanced around the non-enhanced lesions; 4 months later, the size of lesions were relative stable and the periphery enhancement was weaken; 7 months later, the size of lesions were reduced and showed no enhancement. For recurrence lesions, the high signal ring was incomplete on TIWI, the incomplete area showed nodular enhancement on the arterial phase, and most of nodule showed slightly lower signal on the delay phase demonstrated a feature of quick wash-in and wash-out; 7 months after RFA, recurrence rate was 6.12% in patients with complete high signal ring and 43.75% in patients with incomplete high signal ring, the difference was statistically significant (P<0.05). The total survival rate and accumulated survival rate of the patients with complete high signal ring on T1WI were higher than the patients with incomplete ring, the difference was statistically signiifcant (P<0.05).ConclusionThere are characteristic ifndings of MRI examination of liver cancer after percutaneous RFA, observation of the integrity of high signal ring on T1WI image and ifnding of dynamic enhancement scan can early evaluate efifcacy of RFA guide the selection of treatment plan.

ObjectiveTo observe the therapeutic effects of gastric lavage with fuller earth combined with QingyiⅡ catharsis in treatment of oral paraquat poisoning in rabbits.Methods Thirty healthy adult Japanese white rabbits were randomly divided into five groups: namely control group, model group, gastric lavage group (lavage of 10%fuller earth suspension), catharsis group (QingyiⅡ catharsis), and combination group (10 minutes after gastric lavage of fuller earth suspension liquid, giving QingyiⅡ for catharsis), with 6 rabbits in each group. All groups were challenged with paraquat (100 mg/kg) diluted to 5 mL with normal saline by lavage to reproduce the model of acute poisoning, while the control group was given 5 mL of normal saline instead. Each treatment group was treated accordingly at 1 hour after gavages of paraquat, and treatment continued for 3 days. The animal survival rate was observed. Venous blood samples were collected from ear marginal vein to determine the plasma concentration of paraquat by ultraviolet spectrophotometer at 1, 2, 4, 8 and 24 hours after the poisoning. The animals were sacrificed by intravenous air injection on the 8th day after the poisoning, and the right lower lobe of lung was harvested to observe the lung tissue pathological changes with hematoxylin-eosin (HE) staining.Results① Survival rate: the surviving rate of the combination group (6 rabbits) was higher than that of gastric lavage group (5 rabbits), catharsis group (2 rabbits) and model group (0 rabbit) on the 2nd day with statistically significant difference (P< 0.001). The survival rate on the 7th day in combination group (5 rabbits) was higher than that of gastric lavage group (3 rabbits), and catharsis group (0 rabbit) with statistically significant difference (P = 0.003).② Plasma concentrations of paraquat: plasma paraquat concentration in all groups peaked at 2 hours after intoxication, and its levels in the gastric lavage, catharsis and combination groups were significantly lower than that of the model group (mg/L: 1.830±0.068, 1.890±0.048, 1.800±0.052 vs. 1.960±0.063, allP< 0.01). As the time prolonged, the plasma concentration of paraquat was lowest in combination group than that of gastric lavage group and catharsis group (allP< 0.01). Gastric lavage and catharsis had interaction at 4 hours in combination group [F = 5.194,P = 0.034; the concentrations of paraquat (mg/L) was 0.670±0.057 vs. 1.010±0.018, 1.210±0.052].③ Lung histopathology: obvious expansion and hyperemia of the alveolar capillary, widened alveolar septum, a large number of inflammatory cell infiltrations were observed in model group and catharsis group. Lung histopathology was more improved in combination group and gastric lavage group, and it was improved more obviously in combination group than that in gastric lavage group.Conclusions Early start of gastric lavage with fuller earth combined with QingyiⅡ catharsis, can reduce the animal plasma concentrations of paraquat in oral paraquat poisoning rabbits. At the same time, it can alleviate the degree of lung injury and significantly improve survival rates compared with the single gastric lavage or catharsis alone. Gastric lavage with fuller earth combined with QingyiⅡ catharsis can improve the prognosis of animal synergistically.

Objective To explore the clinic value of sequential severity evaluation in emergency nursing for stroke patients. Methods 138 of stroke patients were enrolled in study group, which completed sequential severity evaluation in emergency medical and nursing provision drawn up according to the results. Another 207 of stroke patients were enrolled in control group, which emergency nursing provision drawn up according to general procedure. Length of time from emergency call to special therapeutic, mortality compared between the two groups respectively. Results In study group, length of time from emergency call to special therapeutic (48.9?34.1) min was significantly shorter than that of control group (73.1?46.7) min; mortality (11.1%) was significantly lower than that of control group (24.3%),cure rate (34.7%) was significantly higher than that of control group (26.9%). Conclusion Sequential severity evaluation in acute nursing may be a worthy procedure for proving reaction ability of nurse reaction ability in emergency medical and nursing, proving outcome for stroke patients and it should be commended.

Objective To investigate the causes, clinical features, classifications and liver function change of drug-induced liver damage (DILD) in the elderly. Methods One hundred and sixty seven inpatients with acute drug-induced liver injury in our hospital in the past ten years (January 2000 to December 2009) were retrospectively investigated,and the diagnosis and classification methods of acute DILD were based on international consensus meeting (international criteria). Results Among 167 DILI cases, there were 53 cases (31.7%) in the older group and 114 cases (68.3%) in middle-youth age group. Fatigue and jaundice were the more common symptoms, accounting for 50.3% and 46.7%, respectively. In 167 cases, no obvious symptoms and signs were shown in 25 cases. There were no significance differences in clinical manifestation between two groups. Many drugs could induce liver injury. The most common drugs inducing DILD were Chinese traditional and herbal drugs, accounting for 47.9%. Drugs used in heart diseases and inducing liver injury were more common in the older group. In this study, 40 (75.5%), 5 (9.4%) and 8 (15.1%) cases were designated as hepatocellular, cholestetic and mixed DILD in the older group, and 91 (78.9%), 8 (7.4%) and 15 (13.7%) in middle-youth age group, respectively. There were no significance differences between two groups in classifications. Conclusions Many drugs could cause liver injury. The symptoms of acute DILD are nonspecific. Drugs used in heart diseases and inducing liver injury are more common in older patients.

Aim To prepare a new oral colon-specific delivery formulation and to investigate the release profile in vitro and the colon-specific delivery property in vivo in dogs. Methods Sodium 4-aminosalicylic acid was selected as the model drug. The combination of Eudragit RL30D and RS30D were used as sustained-release film, and Eudragit FS30D used as enteric film, which was expected to release drug depending on pH and time. The release profile of tablets was studied in three phosphate buffers with the pH 6.5, 7.0 or 7.4 for 12 h after a simulated gastric presoak for 2 h in 0.1 mol · L-1 HCl. The tablets were radiolabelled with 99mTc to make their release times and positions in the gastrointestinal tract be followed using a gamma camera. Results For the in vitro study, there was no drug released in 0. 1 mol ·L- 1 HCl for 2 h, and release occurred slowly when pH was above 6.5. Drug was released faster while pH was higher. For the in vivo study, the coated tablets remained intact in the upper gastrointestinal tract, and drug release began after the colonic arrival. The uncoated tablets, however, disintegrated in the stomach of the dogs rapidly. Conclusion The coating could protect the drug until the tablets reached the ascending colon, where drug was released slowly for over 10 h.

Objective To observe the effect of Rood therapy intervened very early on development of premature infants. Methods 148 hospital-born infants gestated 32 weeks with high risk of brain injury were divided into intervention group (n=74) and control group (n=74).All the cases accepted routine treatment and nursing, and the intervention group accepted Rood therapy in addition. They were followed up to 28th day, assessed with Neonatal Behavioral Neurological Assessment (NBNA). Results The NBNA score was more in the intervention group than in the control group (P<0.05) in all the sub-scores except primitive reflexes. Conclusion Rood therapy is useful to improve the neural development in premature infants in 32 to 36 weeks.