Objective To probe into the proper ratio of doctors and nurses and appropriate nursing programs in community health service. Methods A number of methods were used, including task analysis, use of old handsexperience, sociological survey, and literature review. Results Medical consultation took 1 105 minutes, taking blood pressure took 141.5 minutes, transfusion took 493.5 minutes, intramuscular injection took 44.5 minutes, and drawing blood took 77.5 minutes. According to the above results, the ratio of doctors and nurses was respectively 3.18∶1, 0.94∶1, and 2.02∶1. Also, by analyzing in a comparative way the nursing programs already developed both at home and abroad and taking into consideration the reality, suggestions were put forward as to the kind of nursing program communities ought to start. Conclusion The findings are of referential value to administrative departments of health and medical institutions.

Objective To determine the tasks that health human resources of technicians in the medical laboratory specialty ought to fulfill, the factors that affect performance and the estimation of demands. Methods Such methods as analyzing medical records, tasks and health demands were used. Results The current situation of health human resources of technicians is as follows: Those with intermediate, junior or no professional titles respectively account for 46.5%, 36.5% and 11.6%; those with a master's or a bachelor's degree or a degree from a polytechnic college or school respectively account for 2.0%, 8.2%, 42.9% and 40.8%; and others account for 6.1%. The average utilization ratio of laboratory service by inpatients and outpatients is respectively 96.7% and 49.5%; and the average utilization of laboratory service per inpatient and per outpatient is respectively 12.5 and 2.2 times. Of the average daily service volume, emergency, outpatient and inpatient, microbiological, immune serum, and biochemical tests respectively account for 8.3%, 3.0%, 8.1%, 7.8% and 72.8%. The chief tasks of the technicians are performing laboratory operations and assisting the physicians with the reporting of the laboratory results. The demand for health human resources of technicians in the laboratory specialty in the hospitals across the country numbers 89 920 people. Conclusion The results of the study will provide health administrative departments, medical institutions and medical education departments with basis for strengthening human resources management and formulating educational plans.

Objective To observe the practice of speech therapists (ST) in various grades hospitals in China. Methods Speech therapists of 457 hospitals of various grades in 30 provinces, autonomous regions or municipalities were investigated with self-developed question-naire. Results 657 valid questionnaires were returned among 685 questionnaires. The distribution of speech therapists in grades of hospitals was varied with the size of cities (P<0.001), which was concentrated in the grade 3 hospitals in the large cities. For the degree of education, professional background, qualification certificate, employment nature, work time, part-time work, and annual income of the speech thera-pists, it was varied with the grades of hospitals (P<0.05), which trended to be better in the grade 3 hospitals. The first 4 subjects the speech therapists dealt were dysarthria (91.3%), dysphagia (84.7%), aphasia (73.6%) and language development delay (52.7%). Conclusion It is im-portant to support the practice of speech therapists, especially in grade 1 or 2 hospitals in order to match with the international standards.

Objective To construct the has-miR 146a eukaryotic overexpression vector pmR 146a and to explore its effect on the expression of c-Myc gene in HepG2.2.15 cells.Methods The has-miR-146a precursor gene fragment pre-has-miR-146a was amplified by PCR,then connected to the pmR-mCherry plasmid vector after double enzyme digestion,the accuracy of recombinant vector was verified by colony PCR,double enzyme digestion and sequencing;then the recombinant vector was transfected into HepG2.2.15 cells as the experimental group,meanwhile the empty vector group (transfecting pmR-mCherry empty plasmid group) and blank group(transfecting reagent lip2000+PBS),then the fluorescent protein expression amount was observed under the fluorescence microscopy at 24,48 h;the expression of has miR-146a was evaluated by qPCR;at 24,48 h after transfection,the expression levels of c-Myc gene mRNA were detected by qPCR,and the c-Myc protein expression level after 48 h was detected by Western blot.Results The colony PCR,double enzyme digestion and sequencing verified that the pre-has-miR-146a gene fragment was inserted into the pmR-mCherry vector;at 24,48 h after transfection in the experimental group and empty vector group,intracellular strong fluorescence was seen by fluorescent microscope,the transfection efficiency was at 50％-60％ contrasting without fluorescence;the has-miR-146a expression level in the experimental group was significantly higher than that in the empty vector group and blank group (P＜0.01);the c-Myc mRNA expression at 24,48 h after tranfection was significantly lower than that in the empty vector group and blank group (P＜0.05);the protein expression amount at 48 h after transfection was lower than that in the empty vector group and blank group (P＜0.01).Conclusion The pmR-146a eukaryotic overexpression vector is successfully constructed,this recombinant vector can express miR-146a stably;miR-146a can down-regulate c-Myc cancer gene expression,which can serve as one of potential targets for treating hepatocellular carcinoma.