1.Site-specific PEGylation of Engineered Cysteine Analogues of Recombinant Human Interleukin-11
Zhi-Hua LI ; Man-Cang HU ; Ling-Mei YAN ; Yu-Jiao ZHAO ; Xu YANG ; Zheng-Hua PENG ; Wei-Ming XU ; Jian-Feng LI ;
China Biotechnology 2006;0(06):-
Human Interleukin-11(hIL-11)has no Cys residue in its natural form.By site-directed mutagenesis,a Cys residue can be introduced to replace the 1st residue Gly and the rhIL-11 was chemically modified by using 20 kDa mPEG-maleimide conjugated to this site.The mPEG-hIL-11 conjugate was purified and showed a single band on SDS-PAGE with an apparent molecular weight.The biological activity of purified mPEG-hIL-11 was determined using a dependent cell line 7TD1.The remaining biological activity of PEGylated-rhIL-11 was 30% of native rhIL-11,suggesting chemical modification of rhIL-11 by PEG is a promising approach for improving the pharmacological efficacy.
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