Recently, Toll-like receptor 4 (TLR4) passway has been found to play an important role in the inflammation injury after cerebral infarction, and exercise seems to induce a downregulation of TLR4 expression in various tissues. It is possible that TLR4 plays an important role in the mechanism of rehabilitation after a cerebral infarction.

Objective The generation of drug resistance often leads to the failure of the bladder cancer chemotherapy.P-glycoprotein (P-gp) is an ATP-dependent drug efflux pump linked to development of multidrug resistance in cancer cells.The laboratory has successfully established adriamycin-resistant human bladder cancer cell line (pumc-91/ADM) from its parental cell line (pumc-91).According to the drug resistant spectrum analysis,pumc-91/ADM cell line exhibited the characteristics of multi-drug resistance.However,the expression of P-gp in two cell lines was still unknown.In this paper,there was a comparison between pumc-91/ADM and pumc-91 about the differential expression of P-gp.Methods To determine the expression and location of P-gp in pumc-91 and pumc-91/ADM,qRT-PCR,Western blot and immunocytochemistry were applied in the experiment.qRT-PCR was implemented to research the expression of P-gp mRNA in two cell lines (pumc-91/ADM and pumc-91).Western blot was adopted to investigate the expression of P-gp protein in pumc-91 and pumc-91/ADM cell lines.Immunocytochemistry technique was used to explore the cellular location of P-gp and affirm its expression in two cell lines visually.Student's t-test was employed for statistical analysis and P ＜ 0.05 was considered statistically significant.Results qRT-PCR analysis revealed that the expression of P-gp mRNA was upregulated in drug-resistant cell line pumc-91/ADM compared to parental cell line pumc-91.To normalize for differences in the amount of total RNA,GAPDH was selected as an endogenous RNA control.Compared with pumc-91,the expression of P-gp mRNA was upregulated 7.74 fold in pumc-91/ADM (t =11.97,P ＜ 0.05).Consistent with the qRT-PCR result,Western blot confirmed the protein of P-gp expressed differentially in two cell lines.The expression of P-gp protein was significantly increased in pumc-91/ADM compared to pumc-91.According to the results,the differences between pumc-91 and pumc-91/ADM had statistical significance (t =4.35,P＜0.05).Immunocytochemical analysis results demonstrated that P-gp was not only located in cell membrane but also in cytoplasm of the two cell lines.The expression of P-gp in pumc-91/ADM increased distinctly.The difference was statistically significant (t =11.41,P ＜ 0.05).Conclusion Compared with pumc-91,the expression of P-gp in pumc-91/ADM was significantly upregulated.

ObjectiveTo study the personality traits,interpersonal trust and coping style of juveniles in criminal gangs and their relationship. Methods 15 male juveniles who committed crimes without gangs ( group1 ),52 male juveniles who committed crimes by gangs( group2 ) and 40 male lawful freshmen( group3 ) were tested with Eysenck Personality Questionnaire ( EPQ),Trust Scale and Coping Style Questionnaire.The data were statistically analyzed by covariance analysis or Pearson relation analysis.ResultsAfter the covariant effect of education levels was removed,the levels of P and N in EPQ of group1 ( P:57.49 ± 2.62,N:59.35 ± 3.55 ) and group2 ( P:57.83 ± 1.24,N:59.60 ± 1.68 ) were higher than those of group3 ( P:43.88 ± 4.72,N:39.07 ±6.40) with statistical difference (P＜0.05).The level of E of group1 (63.41 ±3.86) was higher than that of group2 (53.01 ± 1.83 ) with statistical difference (P ＜ 0.05 ).The levels of P,D and F in Trust Scale of greup1 (P:24.68 ± 1.51,D:14.36 ±2.19,F:15.49 ±2.21) and group2(P:22.95 ±0.71,D:22.48 ± 1.04,F:23.09 ±1.05 ) were lower than those of group3 ( P:33.14 ± 2.72,D:40.22 ± 3.95,F:38.44 ± 3.99) with statistical difference (P ＜ 0.01 ).The levels of self-accusation and fantasy in Coping Style Questionnaire of group1 ( 0.80 ±0.08,0.83 ± 0.06 respectively) and group2 (group2:0.59 ± 0.04,0.68 ± 0.03,respectively) were higher than those of group3 (0.39 ± 0.14,0.44 ± 0.11,respectively) with statistical difference (P＜ 0.05 or 0.01 ),while the levels of problem-solving of group1 ( 0.76 ± 0.06 ) and group2 ( 0.70 ± 0.03 ) were lower than that of group3 ( 0.95 ± 0.11 ) with statistical difference (P ＜ 0.05 ).For the male juveniles who committed crimes by gangs,the P of personality traits had a statistical linkage with the problem-resolving and self-accusation of coping style ( r =- 0.389,- 0.395,P ＜ 0.05 ),the N of personality traits had a statiseical linkage with the F of interpersonal trust and the self-accusation of coping style ( r=-0.473,0454,P ＜ 0.05 ),and the E of personality traits had a statistical linkage with the help-seeking of coping style ( r=0.400,P＜ 0.05 ).ConclusionThe personality traits,interpersonal trust and coping styles of male juveniles who committed crimes with or without gangs and the lawful men are different.These psychological factors may have certain effect on juvenile gang crimes.

AIM: To compare the efficacy and safety between quetiapine and clozapine. METHODS: There were 85 patients with first on-set schizophrenia in this study,43 patients treated with quetiapine 425? 56 mg?d -1 and 42 patients treated with clozapine 350? 73 mg?d -1 . The study lasted for 8 wk. The Positive and Negative Symptom Scale (PANSS) and Treatment Emergent Symptom Scale (TESS) were used to evaluate therapeutic and side effects. RESULTS: The PANSS scores in both groups decreased significantly after treatment with those two drugs(P

OBJECTIVE To investigate flora distribution and drug resistance status of hospital acquired infection with Gramnegative bacilli in lower respiratory tract in intensive care unit(ICU) and to provide the scientific reference for diagnosis and reasonable use of antibiotics.METHODS Referring to National Rules of Procedures in Clinical Laboratory,the strains were isolated.The strains were identified by Identification System of Microscan auto-4. The antibiotic susceptibility test was performed by K-B method and the results were read according to CLSI 2007.RESULTS Among 319 strains of Gram-negative bacilli,the top 4 of isolated rate were Pseudomonas aeruginosa(23.5%),Klebsiella pneumoniae(17.5%),Acinetobacter baumannii(14.4%),and Escherichia coli(14.1%).Severe drug resistance was found in four kinds of important pathogens.Except for 100% susceptibility of Enterobacteriaceae to carbopenems,the drug resistance rate to carbopenems in P.aeruginosa and A.baumannii reached 19.6%-22.7%.Pan-drug resistant strains were found in A.baumannii and P.aeruginosa strains.The detection rate of extensive-spectrum beta-lactamases(ESBLs)-producing K.pneumoniae and E.coli was 39.3% and 42.2%,respectively.CONCLUSIONS The Gram-negative bacilli isolated from lower respiratory tract in hospital acquired infection in ICU are mostly multi-resistant.We should strengthen monitoring and controlling of them.

Objective: To observe therapeutic effects of exercise rehabilitation therapy in patients with chronic heart failure (CHF). Method: A total of 60 CHF patients were randomly divided into routine treatment group (n=30) and exercise rehabilitation group (n=30, received exercise rehabilitation therapy based on routine anti heart failure drug treatment). Cardiac function, exercise capacity, 6min walking distance and rehospitalization rate of the patients were observed， therapeutic course was three months. Result: Total effective rate in exercise rehabilitation group was significantly higher than that of routine treatment group (93.3% vs. 80.0%, P<0.05). Compared with routine treatment group after treatment, there was significant improvement in clinic cardiac function [NYHA class: (1.96±0.89) classes vs. (1.48±0.72) classes, P<0.05], significant increase in exercise capacity [(4.01±0.82) METs vs. (5.24±1.16) METs] and 6 min walking distance [(322.5±23.4) m vs. (413.6±26.5) m, P<0.01 both] and significant decrease in rehospitalization rate (56.7% vs. 30.0%, P<0.05). Conclusion: Exercise rehabilitation therapy can improve cardiac function, increase exercise capacity and decrease rehospitalization rate in patients with chronic heart failure.

Objective To develop a rapid and sensitive liquid chromatography-tandem mass spectrometric(LC-MS/MS) method for the determination of valproic acid in human plasma.Methods After treating human plasma sample by acetonitrile protein precipitation method,the analytes were separated on a Shimpack VP-ODS analytical column(150 mm×2.0 mm I.D,5 μm) with the mobile phase of methanol and 5 mmol/L ammonium acetate (55∶45,v/v)at a flow rate of 0.4 mL/min.Detection was carried out by adopting the multiple reaction monitoring(MRM) scanning mode in the API3200 triple quadrupole tandem mass spectrometer,electrospray ionization source,negative ion mode,selected monitoring ionic reactions were m/z 142.9→m/z 142.9(valproic acid) and m/z 179.0→m/z 179.0(1-sulfonic acid).Results Valproic acid and internal standard 1-sulfonic acid retention time were 3.03 min and 2.38 min respectively.The plasma valproic acid linear range was 0.800-80.0 μg/mL(r>0.99) with the lower limit of quantitation(LLOQ) 0.800 μg/mL.The intra-and inter-batch relative standard deviations(RSD) were both less than 15%,and the relative errors(RE) were within ±15%.The mean extraction recovery rate was(84.1±2.4)%,and the mean matrix effect factor was(104.3±2.0)%.In the stability study,valproic acid was found to be stable in plasma under various storage conditions.Conclusion This method is suitable for the determination of valproic acid in human plasma and human pharmacokinetic study of valproic acid semisodium sustained release tablet.

Objective To observe the changes of PECAM-1,Bcl-2 and Bax expressed by ischemic cerebrum of adult rats after focal cerebral ischemia. Methods Middle cerebral artery occlusion (MCAO) models were made by operation with Longa suture method in Wistar rats. The expression levels of PECAM-1,Bcl-2 and Bax at the 6th,12th,18th,24th,48th,72nd hour after MCAO were detected by immunohistochemistry staining. Results The expression levels of PECAM-1,Bcl-2 and Bax in cerebrum were significantly increased after MCAO. The expression levels of Bcl-2 we、re up to the peak at the 12th hour after MCAO, while the levels of Bax and PECAM-1 were up to the peak at the 24th and 48th hour after MCAO. At the 72nd hour after MCAO, the expression levels of PECAM.1.Bcl-2 and Bax were still higher than that in the control group(all P＜0.001).Conclusions PECAM-1,Bcl-2 and Bax participate in the different pathological stages of focal cerebral ischemia.

Objective To explore the effect of positive end expiratory pressure (PEEP) on arterial oxygenation and intrapulmonary shunt during one-lung ventilation (OLV) and pulmonary function during perioperation. Methods Forty patients with normal pulmonary function,ASA I - II :scheduled for pulmonary lobectomy, were divided into control group and PEEP group by random digits table with 20 cases each. Patients were induced by double-lumen tubes under intravenous anesthesia and were received 10 ml/kg tidal volume, 12 frequents/min breathing rate during the two-lung ventilation (TLV), secondary reduced to 6 ml/kg tidal volume, 16-18 frequents/min breathing rate without PEEP (control group) or with 5 cm H2O cm H2O =0.098 kPa) PEEP (PEEP group) during OLV.Hemodynamics and respiratory mechanical parameters were continuously monitored, lung function before operation and at 72 h after operation was detected. Results Compared to before OLV,arterial oxygen tension (PaO2), arterial oxygen saturation (SpO2), oxygenation index (OI) were decreased and intrapulmonary shunt ratio (Qs/Qt) was increased in control group and PEEP group at 30 min after OLV (P < 0.01 or < 0.05). However,PaO2 and SpO2 and OI were higher and Qs/Qt was lower in PEEP group than that in control group at the same time point (P<0.05). In addition, FEV1%, FVC% and FEV1/FVC were (121.8 ± 25.0% ,(117.2 ± 24.3)% , (87.6 ± 15.7)%before operation and (84.9 ± 21.6)%, (77.2 ± 18.3)% , (70.5 ± 12.5)% at 72 h after operation respectively in control group, (116.9 ±24.5)% , (112.1 ±23.6)% , (85.3 ± 13.8)% before operation and (96.3 ± 20.4)%, (88.1 ± 19.8)% , (78.4 ± 10.2)% at 72 h after operation respectively in PEEP group. Although decreased in control group and PEEP group at 72 h after operation comparing with preoperation (P< 0.01 or < 0.05 ), FEV1%, FVC% and FEV1/FVC were higher in PEEP group than those in control group at 72 h after operation (P<0.05). Conclusion Appropriate PEEP increases arterial oxygenation,reduces Qs/Qt and improves pulmonary function during OLV,reduces the risk of hypoxernia and lung injury induced by OLV during perioperation.

Objective To investigate the placenta perfusion in pre-eclampsia by three-dimensional power Doppler ultrasound and analysing the clinical outcome. Method 80 cases of normal and pre-eclampsia pregnant women from July 2007 to May 2008 in Beijing Obstetrics and Gynecology Hospital were studied. The control group of 36 cases, pre-eclampsia were divided into three groups: a total of 44 cases, mild pre-eclampsia group (9 cases), severe pre-eclampsia group (26 cases) and chronic hypertension with pre-eclampsia group (9 cases). The placental vascular index (VI), flow index (FI), blood vessels and blood flow index (VFI) by three-dimensional Doppler histogram and the umbilical blood flow systolic to diastolic (S/D) by color Doppler flow imaging were calculated and the gestational age after delivery, birth weight, placental weight after birth were recorded respectively. Results (1)VI、 FI、 VFI and umbilical blood flow S/D value :6.3±2.9, 38.6±4.4, 2.7±1.3, 2.5±0.6 in normal group ;5.7±3.8, 36.3±7.2, 2.4±2.0, 2.4±0.3 in mild pre-eclampsia group;3.0±2.4, 31.7±5.0, 1.1±1.0, 2.9±1.3 in severe pre-eclampsia group ;2.2±1.6, 26.1±4.4, 0.8±0.6, 3.1±1.6 in chronic hypertension with pre-eclampsia group. Placenta of normal group and mild preeclampsia group VI, FI, VFI were no significant difference (P>0.05), placental VI, FI, VFI of severe preeclampsia group and chronic hypertension with severe pre-eclampsia group were significantly lower than the normal group (P<0.01) and than mild pre-eclampsia group (P<0.05). The umbilical blood flows were not obvious difference among four groups (P>0.05) ; (2) Gestationul age after birth, birth weight, low newborn weight rate and placental weight:(38.7±1.5 ) weeks, (3280±520) g, 3%, (568±141) g in normal group; (37.9±1.0) weeks, (2971±265) g, 0,(576±98) g in mild pre-eclampsia group; (33.2±2.6) weeks, (1820±737) g,58%, (458±154) g in severe pre-eclampsia group; (32.6±2.6) weeks, (1497±533) g, 7/9, (396±141) g in chronic hypertension with pre-eclampsia group. The normal group and mild pre-eclampsia group in gestationul age after birth, birth weight, low newborn weight rate and placental weight were not significant difference (P>0.05); severe pre-eelampsia and chronic hypertension with severe pre-eclampsia group in them were significantly lower than the normal group (P<0.01) and than mild pre-eclampsia group (P<0.05).Conclusions (1) Placental blood flow perfusion of the severe pre-eclampsia and pre-eclampsia with chronic hypertension of pregnancy decreased resulting in clinically lower placental weight, birth weight and gestational age at delivery, but there were no obvious differences in umbilical blood flow S/D values. (2) The investigation was helpful to clinical diagnosis in the placenta perfusion of pre-eclampsia by three-dimensional power Doppler ultrasound.