1.Purification of methanol dehydrogenase from mouth methylotrophic bacteria of tropical region
Diana Waturangi ; Nico Marko ; Maggy Thenawidjaja Suhartono
Malaysian Journal of Microbiology 2011;7(4):226-229
Aims: Purification of methanol dehydrogenase (MDH) from methylotrophic bacteria was conducted to obtain pure enzyme for further research and industrial applications due to the enzyme’s unique activity that catalyzes oxidation of methanol as an important carbon source in methylotrophic bacteria.
Methodology and Results: The enzyme was screened from methylotrophic bacteria isolated from human mouth. Purification of this enzyme was conducted using ammonium sulphate precipitation followed by cation exchange chromatography. Two types of media were used to produce the enzymes: luria broth and standard mineral salts media (MSM). MSM produced MDH with higher specific activity than LB. Specific activity was also increased along with the purification steps. Application of ammonium sulphate increased the purity of enzyme and was more effective for the enzyme produced in LB. Using sepharose increased the enzyme activity 10 -57 folds.
Conclusion, significant and impact of this study: With this, ammonium sulphate precipitation coupled with single cation exchange chromatographic system has been proved to provide sufficient purified of methanol dehydrogenase from methylotrophic bacteria origin of human mouth with high specific activity for further application.
2.Characterization and identification of mannanolytic actinomycete Nonomuraea sp. ID06-379
Shanti Ratnakomala ; Yopi ; Bambang Prasetya ; Maggy Thenawidjaja Suhartono ; Anja Meryandini
Malaysian Journal of Microbiology 2015;11(3):284-293
Aims: The study focused on screening, identification and characterization of mannanolytic actinomycetes isolated from
soil and leaf litter samples obtained from several sites in Indonesia.
Methodology and results: A total of 337 isolates of actinomycetes isolated from soil and leaf litter samples collected
from various areas in Indonesia were screened for their mannanolytic activity. Mannanase activity was analysed using
locus bean gum (LBG) as the substrate. The strain ID06-0379 displayed significant mannanase activity. The strain ID06-
0379 was analysed for its mannanase activity by determining the rate of enzyme production when cultured in the
presence of palm kernel cake (PKC) as a substrate. The highest mannanase activity from ID06-0379 was 4.40 U/mL at
5% PKC concentration at 5 days incubation. Chemotaxonomic and phenotypic characterisation of mannanolytic
actinomycete was done and the strain ID06-0379 contained meso-diaminopimelic acid, and madurose was the
diagnostic sugar in whole cell sugar. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol,
phosphatidylinositol, and hydroxy-phosphatidylethanolamine. The predominant menaquinone of strain ID06-0379 was
MK-9(H4). The major cellular fatty acids were C16:0 (31.47%), cis9-C16:1 (15.23%) and iso-C16:0 (10.84%), and the G+C
content of the DNA was 71.7 mol%. Phylogenetic analysis based on the 16S rDNA sequences revealed that strain ID06-
379 was closely related to species of Nonomuraea jabiensis A4036T with 99% nucleotide similarity.
Conclusion, significance and impact study: The results from this study revealed that the mannanolytic actinomycete
strain ID06-379 belongs to the genus Nonomuraea that closely related to N. jabiensis A4036T
. Mannanase production
using agricultural waste such as palm kernel cake may contribute to the development and utilisation of biomass
bioconversion processes.
Keywords: Indonesian actinomycetes, mannanase enzyme, locus bean gum, palm kernel cake, Nonomuraea sp. ID06-
0379.
3. Potential effect of striatin (DLBS0333), a bioactive protein fraction isolated from Channa striata for wound treatment
Puji RAHAYU ; Faustine MARCELLINE ; Erna SULISTYANINGRUM ; Maggy Thenawidjaja SUHARTONO ; Raymond Rubianto TJANDRAWINATA ; Maggy Thenawidjaja SUHARTONO
Asian Pacific Journal of Tropical Biomedicine 2016;6(12):1001-1007
Objective To characterize proteins and other nutrients in striatin (DLBS0333), a bioactive protein fraction isolated from snakehead fish (Channa striata) and to investigate its wound healing activity. Methods Proteins and other constituents in striatin were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two dimension electrophoresis, immunoblotting assay, spectroscopy and high performance liquid chromatography. The wound healing activity of striatin was studied in vitro using 3T3 fibroblast cells and in vivo using wound-induced animal model. Various parameters related to wound healing process were evaluated. Results Striatin contained four major bioactive proteins with approximate molecular weight of 8.3, 10.9, 15.4 and 16.7 kDa. In addition to proteins, striatin also contained amino acids (10 essential and 7 non essential amino acids), fatty acids (palmitic acid, oleic acid, stearic acid, linoleic acid, arachidonic acid), vitamins (vitamin A, vitamin B
4.Suppression of Feed Intake in Response to Rice Bran Oil Supplementation in Normal Rat
Evy Damayanthi ; Hana Fitria Navratilova ; Maggy Thenawidjaja
Malaysian Journal of Medicine and Health Sciences 2020;16(Supp 6, Aug):184-190
Introduction: Rice bran oil (RBO) is used in Asian countries as a daily dietary supplement. RBO is known in particular for its hypolipidemic effect. There has been increasing interest recently in the use of RBO as a means to maintain body weight and prevent obesity, though the mechanism of how this happens is still not well understood. We have investigated the effect of RBO on expression of genes that might influence energy homeostasis and feed intake. Methods: This study assessed Sprague-Dawley male rats at 12-weeks that were split into three groups over a 28-day period. A control group was fed a diet of standard rat chow, a standard group was fed standard rat chow with Orlistat (10.8 mg/kg bw/day), and a treatment group was fed standard rat chow with RBO (57.6 mg oryzanol/day). All supplementation was given by oral gavage. Possible adiposity was investigated through a histological analysis of adipocytes size measurement of intra-abdominal white adipose tissue in the rats. Changes in gene expression in the liver were determined by microarray. Results: The data suggest that RBO supplementation of a regular diet did not result in excess body weight and adiposity. A microarray analysis of the rats’ livers found that RBO altered the expression of genes related to energy homeostasis and feeding behavior, by upregulating genes such as Olr522, RGD1561231 and Rgs16. Conclusion: It is suggested that RBO supplementation can be used to maintain body weight by lowering appetite.
5.Lytic polysaccharide monooxygenase of soil actinomycete with potential use for lignocellulose biodegradation
Esti Utarti ; Antonius Suwanto ; Maggy Thenawidjaja Suhartono ; Anja Meryandini
Malaysian Journal of Microbiology 2021;17(1):60-68
Aims:
Lytic polysaccharide monooxygenase (LPMO) is an enzyme capable of cleaving glycoside bonds of recalcitrant
polysaccharides through an oxidative mechanism. LPMO activity, in synergy with hydrolytic enzymes, increases the
production of monomer sugars from the biodegradation of lignocellulose. This study was aimed at evaluating
actinomycete S2 strain LPMO activity based on the release of xylose as one of reducing sugar and hydrogen peroxide
(H2O2) in the course of lignocellulosic biodegradation.
Methodology and results:
The oxidation activity of LPMO from actinomycete S2 strain was measured by using the
substrate of Avicel supplemented with ascorbic acid and copper ions (Cu2+) to identify its effect on the release of xylose
as one of reducing sugar. The optimum incubation time for the LPMO production was also conducted. Further, H2O2
quantitative analysis was performed as by-product of LPMO activity and 16S rRNA gene sequence of actinomycete S2
strain were subsequently determined. We found that supplementation of 1 mM ascorbic acid and 0.2 mM Cu2+ increased
xylose as one of reducing sugar production by up to 5-fold from 255.03 to 1290 μg/mL after an optimal incubation period
of 6 days. Based on H2O2 production, the LPMO activity of actinomycete S2 strain was 0.019 ± 0.001 U/mL. There is
likelihood that LPMO activity derived from actinomycete S2 strain has a synergistic effect with the activity of other
lignocellulose-degrading enzymes. This actinomycete showed 99% similarity to the 16S rRNA gene sequence of
Streptomyces avermitilis strain EAAG80.
Conclusion, significance, and impact of study
LPMO enzyme activity from actinomycete S2 strain as determined by
the production of reducing sugar and H2O2 was greatly increased by supplementation with ascorbic acid as an electron
donor and Cu2+ ions. To the best of our knowledge, this is the first elucidation of LPMO activity from an indigenous
Indonesian actinomycete.
Mixed Function Oxygenases--metabolism
;
Lignin--metabolism