Aim Subcortical ischemic vascular demen-tia ( SIVD ) induced by chronic hypoperfusion due to small-artery disease is a common cause of vascular de-mentia ( VaD) , which is recognized as the second most prevalent type of dementia. The aim of this study was to determine whether carnosine played a protective role in cognitive impairment induced by permanent occlu-sion of the right unilateral common carotid arteries ( rUCCAO ) in SIVD. Methods Adult male mice ( C57BL/6 strain ) were subjected to rUCCAO, and treated with carnosine or saline. Locomotor test, open field test, hot plate test, freezing test and Morris water maze were performed after rUCCAO. Results There were no differences among rUCCAO group, carnosine group and sham group for total distance traveled in lo-comotor test. In the open field test, carnosine (200, 500 mg · kg-1 ) significantly revised the decrease of latency spent in the center induced by SIVD . There were no differences between rUCCAO and sham groups for the pain threshold. In freezing test, rUCCAO in-duced a significant reduction in content memory, which was completely reversed by treatment of carnosine. In Morris water maze training trials, rUCCAO-treated mice showed prolonged escape latency in acquisition phase, carnosine ( 200, 500 mg · kg-1 ) markedly shortened the escape latency. Conclusion These data suggest that carnosine has a neuroprotective effect on cognitive impairment induced by rUCCAO in mice.

Guided bone regeneration technique (GBR) is regarded as one of the most important methods to increase the bone amount of alveolar ridge, which is often recommended to be applied in the dental implantation surgery. Barrier membrane is utilized in GBR technique to cover the bone defect and create a secluded space, which prevents the connective tissue from growing into the space and facilitates the growth priority of bone tissue. In recent years, along with the emerging of different kinds of barrier membranes and bone grafting materials, the scope of clinical application of this technology is continuously expanded. This article reviews the classification and property of the membranes and the application situation of guided bone regeneration technology in the field of dental implantation.

Objective To study a rapid method for determination of trace formaldehyde in drinking water by oscilloscopic polarography.Methods In the base solution of0.01mol/L H 3 PO 4 ,the reaction product of formaldehyde and phenylhydrazine hydrochloride on the drop mercury electrode revealed a sensitive second order derivative polarographic wave at a pick electric potential-0.76V(VS?SCE).The optimum conditions and interference by other coexisting ions were analyzed.Results The de-tection limit,linear range,recovery rate,relative standard deviation(RSD)of the method were0.002mg /L,0.005-0.25mg /L,94.0%-103.0%,and0.05).Conclusion The method was simple,rapid,sensitive and highly specific.The analytical speed was about 50-60samples /h,which was suitable for the determination of trace formaldehyde in drinking water.

To investigate the correlation between the expression of vascular growth factor (VEGF) and its receptor KDR and angiogenesis in human colorectal cancer. VEGF and KDR were detected in 68 cases colorectal cancer by immunohistochemical SABC technique. Microvessel density was determined by immunostaining for factor Ⅷ related antigen. Results showed that the positive rate of VEGF was 55 9% (38/68) in 68 cases of colorectal carcer, and VEGF was mainly located at the cytoplasm or the membrane of the colorectal cancerous cell; KDR positive rate was 45 6% (31/68), and KDR was located in the vascular endothelial cell of colorectal cancer tissues and their related Peri Cancerous tissues but also in the cytoplasm or the membrane of the colorectal cancerous cell. VEGF was closely related to Dukes stage. Microvessel density (MVD) was significantly higher in VEGF positive tumor than in VEGF negative tumor ( P

BACKGROUND:Periodontal tissue engineering technology provides new ideas and new ways for periodontitis-induced bone defect repair. OBJECTIVE:To develop a culture model for the periodontal ligament cells of miniature swine, which was constructed with hydroxyapatite, to investigate the biocompatibility with hydroxyapatite. METHODS:Periodontal ligament cells from miniature swine were harvested by using tissue explant method. Immunofluorescence was used to detect the expression of stromal cel antigen 1 in the periodontal ligament cells of miniature swine. The third passage cells were co-cultured with a three-dimension hydroxyapatite scaffold, and the biological characteristics of the cells were observed under a scanning electron microscope at days 1, 3, 7 of co-culture. RESULTS AND CONCLUSION:The pirmary miniature swine periodontal ligament cells grew wel , and they were positive for stromal cel antigen 1. Under the scanning electron microscope, the periodontal ligament cells of miniature swine grew wel on the hydroxyapatite scaffold at days 1, 3, 7 of co-culture. These prove that the miniature swine periodontal ligament cells, which can be separated using tissue explant method and cultured successful y in vitro, can grow wel on the hydroxyapatite scaffold.

To identify Taenia cestodes from 6 regions of Yunnan province by PCR and sequencing of mtCOXⅠfragment. the genomic DNA of Taenia cestodes was extracted from proglottid collected in 6 region of Yunnan province, and mtCOXⅠ gene fragments were amplified by PCR, and then sequenced. The genomic distance and phylogenetic tree were constructed in comparison with other known mtCOXⅠgene sequences of T.solium , T.saginata and T. asiatica in GenBank using DNA MAN software. Through distance matrix,it was found that the homologie of NJ4, NJ1 and DQ2 was 99.8%, DL4 and NJ3 homologie was 99.5%, NJ2 and DQ3 homologie was 98.8%; the homologie of DL3 and BZ3 was 98.3%, while the homologie was 96.0% with BZ2; The phylogenetic tree demonstrated that 10 Taenia cestodes including NJ1-4, DL2-3and DQ1-3 occupied one brance with BZ3. BN1, CX1, LC1 and BZ2 occupied one brance, then two brance occupied and occupied with other one which was occupied by DL1 and BZ1. Taenia cestodes from Nujiang and Diqing were T. asiatica. Taenia cestodes from XiShangbanna,Lincang and Chuxiong were T.saginata.Taenia cestodes from Dali were T.solium or T.asiatica.Because same species have no difference from different regions. mtCOXⅠfragment sequencing is valid for tapeworms identification.

Objective To study the action of Mycoplasma genitulium (Mg) in pathogenesis of chronic non-bacterial prostatitis. Methods Mg culture and polymerase chain reaction (PCR) were perfomed on prostatic secretion specimens from 987 patients with chronic non-bacterial pristatitis and 125 healthy men. Results ①The positive rate of Mg in the prostatic secretion from the 987 patients was 31.5% (302/987). Among the 302 positive cases, 153 cases had other pathogens, of which Ureaplasma urealyticum (Uu) was the most (128). ②In the controls, the positive rate of Mg was 2. 53% (3/125). The difference between the two groups was statistically significant (X2=44. 32,P

OBJECTIVE To investigate the distribution and the drug resistance of pathogens in cerebral stroke patients with hospital infction and to provide a foundation for reasonabie application of antibacterials.METHODS Pathogenic bacteria were isolated from inpatients of burn department from 2003 to 2008,instruments assay or K-B disk diffusion method was taken to carry out the sensitive test.The drug resistance rate of the pathogenic bacteria was analyzed.RESULTS Totally 222 strains were isolated,among which 122 strains were Gram-negative bacteria,58 strains were Gram-positive ones,and 42 strains were fungi.The percentage of these three groups was 54.95%,26.13% and 18.92%,respectively.The main strains of Gram-negative bacteria were Pseudomonas aeruginosa,Escherichia coli,and Enterobacter cloacae,and that of the Gram-positive bacteria were Staphylococcus aureus,Enterococcus and S.epidermidis.The major strain of fungi was Candida albicans.The pathogenic bacteria tested showed high drug resistance.The detection rate of the meticillin-resistant S.aureus(MRSA)was 73.68%.The detection rate of the ESBLs from the K.pneumoniae and the E.coli was 60.00% and 33.33 %.CONCLUSIONS The pathogenic bacteria are multidrug-resistant.Inspecting pathogens and strengthening susceptibility tests are very important in reducing drug abuse,decreasing the resistance rate and raising the cure rate in hospital.

In healthy control group(group A),impaired fasting glucose group (group B) and diabetes group(group C),serum ferritin levels gradually raised[(170.7±91.8),(255.2±161.9) and (362.8±298.5) μmol/L].Hemoglobin Was elevated in impaired fasting glucose group,while returned to normal or even lower in diabetes group[group A(141.0±9.5),group B(148.2±11.2),group C(132.3±19.2)g/L].There was no difference in high sensitivity C-reactive protein among the groups.

OBJECTIVE:To study the correlation between antibacterials amount and drug resistance of Echerichia coli,and to provide reference for clinical use of antibacterials. METHODS:Retrospective review was used to calculate DDDs of antibacterials and resistance rate of Escherichia coli to 11 kinds of antibacterials each quarter. The correlation analysis was carried out using the SPSS 13.0 statistical software. RESULTS:The resistance rates of E. coli to piperacillin/tazobactam,cefoperazone/sulbactam and le-vofloxacin were with upward trends,and the others showed downward trends. The resistance rates of E. coli to meropenem and imi-penem/cilastatin appeared in 2014,increasing from 0 to 8.8% and 9.4%,respectively. DDDs of them were significantly correlated to drug resistance of E. coli,showing positive correlation(r=0.915,0.793,P<0.01). DDDs of piperacillin/tazobactam was signif-icantly correlated to resistance rate of E. coli(r=0.807,P<0.01),while that of ceftazidime was negatively correlated to resistance rate of E. coli(r=-0.672,P<0.05). There was no statistical significance in resistance rate of E. coli to other 7 kinds of antibacte-rials. CONCLUSIONS:There are some correlations between the DDDs and resistance rates. We should strengthen the monitoring of bacterial resistance and the management of rational application of antibacterials.