Objective: As the breakthrough for public hospital reform in China, County public hospital comprehensive reform has become the main part of the reform, the reasonable use of New Rural Cooperative Medical System(NCMS) would strongly push the process of new medical reform. Methods: The method of mathematical modeling was applied in policy imitate of hospital operation historical data, to estimate the increasing of decreasing status of NCMS hospital compensation expenditure after the implementation of county-level public hospital comprehensive reform measurement in 10 county-level public hospital. Results: Through analyzing the operation data of applying county-level public hospital comprehensive reform measurement in 10 county-level public hospital from 2010 to 2012, there was 66.67% annual time, the NCMS compensation expenditure for public hospitals increased to 23.075 6 million yuan, which increased by 1.691 7 yuan averagely. Conclusion: After the implementation of comprehensive reform policies, the influencing factors of NCMS funds on the decreasing degree of county-level public hospital expenditure were mainly the proportion of pre drug addition ratio and basic drug sales in the total drug sales.

Survivin is the strongest member of the inhibitor of apoptosis protein(IAP)family. Smac is the second mitochondria-derived activator of cysteine proteases,which can promote apoptosis by combining with IAP. Abnormal expression of them is closely related with occurrence,development,treatment tolerance and prognosis of ovarian cancer. It is prompted that Survivin and Smac are expected to play important roles in the early diagnosis and targeted therapy of ovarian cancer.

Objective To study the curative effectiveness of external iliac-popliteal artery artificial graft cross bypass in treating extensive arterial occlusion in unilateral lilac-femoral arteriosclerosis. Methods From Sep. 1999 to Oct. 2007, 39 elder patients were diagnosed as having extensive arterial occlusion in unilateral lilac-femoral arteriosclerosis by color Doppler ultrasonography, CTA or DSA. 25 patients (61.5%) had resting pain, 14 had ulcers or gangrenes in the toes. The average ankle-brachial index was 0. 19. Contralateral external iliac-popliteal artery artificial graft cross bypass surgeries were performed on 22 patients from left to right, the other 17 were from right to left. Results There were no perioperative death or amputation. The ankle-brachial index increased from 0 ～ 0. 41 ( preoperative ) to 0. 85 ～ 1.02(postoperative) ; the claudication distance increased from 15 ～ 60 m (preoperative) to 350 ～ 500 m or more than 500 m [ postoperatively at the speed of (100 ～ 120) m/min] ; The postoperative color Doppler uhrasonography showed the average velocity of the popliteal artery was 45 cm/s. In the anterior tibial artery or the posterior tibial artery, little blood flow was showed in preoperative color Doppler ultrasonography examination, the postoperative average blood flow velocity was 41 cm/s. 35 patients (89. 7% ) were followed up for an average of 3.4 years, with an accumulative patency rate of 85.7%. Four patients had amputation, the limb salvage rate is 88.6%. Conclusion External iliac-popliteal artery artificial graft cross bypass is effective in treating unilateral lilac-femoral arterial occlusion, particularly for aged patients with vital organ incompetence.

Objective To investigate the effect of arsenic trioxide (As2O3) and imatinib mesylate (imatinib) or in combination with imatinib with different concentration on Raji cells and the mechanisms.Methods The inhibition of cell proliferation was measured by MTT assay to assess the cells survival after As2O3 and imatinib or in combination with imatinib treatment with different concentration at indicated time.Caspase-3 activity changes and the relative number of cells in different phases and the percentages of cells calculated in G1 and S phase of the cell cycle were assessed by FCM. The expression of p16 protein was analyzed by SABC immunohistochemical method. Results The results of MTT assay showed that As2O3 and imatinib or in combination with imatinib could inhibit Raji cells growth. There was clear statistical significance between the union groups and the single-drug group (P<0.05). As2O3 inducing apoptosis was accompanied by up-regulation and activation of apoptosis protein Caspase-3. The mean percentage of apoptosis cells was both in time-and dosage-dependent form (P<0.0001). While Raji cell lines were less sensitive to imatinib than arsenic trioxide (P >0.05). Further more, Combination of imatinib with As2O3 had not a synergistic inducing apoptosis effect on Raji cells (P >0.05). The optical density of p16 protein increased both in time-and dosage-dependent form with As2O3 by immunohistochemical method (P<0.05). While at higher concentration and for a longer time, imatinib could up-regulated the optical density of p16 protein. There was no obvious statistic significance for p16 protein in Raji cells with using As2O3 only compared with the unions group(P >0.05). The cell cycle was arrested at G1 phase, the number of cells G1 period increased significantly,and S phase decreased on Raji cells after As2O3 treatment. The relationship between the cellular DNA contents and the concentration of As2O3 showed a dose-and time-dependent manner (P<0.0001). But it was found that imatinib had no effect on Raji cell cycle. There was no the obvious difference (P >0.05) between two drugs unions group with only using As2O3 group. Conclusion The results showed that As2O3 exerted variable and definite effects on lymphoma Raji cells, and suggested that As2O3 might induce apoptosis and up-regulate the optical density of p16 protein and arrest cell cycle. As for Raji cells, imatinib might affect the expression of p16 protein at higher concentration. Two drugs unions had no effective and synergistic effect.

Radiotherapy is an important treatment for malignant tumors, but it will have a negative impact on the reproductive function of young female patients.In recent years, the measures to protect female fertility are increasing, including clinical mature technologies such as embryo cryopreservation and mature oocyte cryopreservation; highly experimental technologies such as immature oocyte in vitro fertilization and ovarian tissue cryopreservation and transplantation.In addition, the application of stem cells and the proposal of artificial ovary also show great prospects in the protection of female fertility.This paper reviews the research progress of female fertility protection strategies in radiotherapy.

To elaborate the principal pathogenesis is that imbalance of zang-fu organs and disorder of qi lead to internal wind ,which instigates pathogenic phlegm ,fire and blood stasis to the lesion of brian function.Then to advocate applying Chinese medicine intervening timely the treatment,so as to dispel pathogeny and recuperate the function of zang-fu organs. Moreover to illustrate the concrete clinical adhibition and do some salutary exploration particularly in the aspect of decreasing the number of outbreak.

Developing along with the chemicals to tend gradually hard and adopting the natural medicine to rise,the international pharmacy field interesting in Chinese materia medica(CMM) increases gradually.The exterior competition of the CMM enterprises is becoming drastic and the secondary deve-(lopment) of CMM for the medicine enterprises in our country should be a better choose to face the international medicine market competition.The internal and external process about the secondary development of CMM should be taken into account and the strategy that should be adopted in the secondary development put forward.Such as: secendary development of new medicine of the CMM compound recipe;turning deeply to the CMM foundation research;the importance of developing the safe and valid CMM whose qua-(lity) is stabile and controlled;the importance of rising the processing level of the product,etc.It emphasized the importance in appling modern science and technology in the text.

[Objective] To dicuss the application of contraray treatment from“Yel ow Emperor’s Internal Classics”in gynecological diseases, hoping to guide the clinic. [Method] By reviewing the articles concerning contrary treatment in “True Large Theory.Yel ow Emperor’s Internal Classics”, take clinical recipe cases as examples, such as stimulating the menstrual flow and water circulation for long menstruation; dispel ing wind to relieve exterior syndrome for night sweat in menstruation;dismounting for morbid leucorrhea;reinforcing spleen for pregnant digestion;tonifying Qi for pregnant constipation;warm drugs for sore throat, etc. Meanwhile, it sums up the application rules of the contrary treatment in gynecological diseases. [Result] The gynecological dis-eases can be cured by contrary therapy based on differentiation of signs, i.e.treating diarrhea with purgative,treating obstruction with tonics, treating heat with warm, al with good cure effect proved by practice.[Conclusion] To treat gynecological diseases with contrary therapy is the demonstration of search-ing for primary cause in treatment;we shal further dig out, inherit and develop it and make it better serve the clinic.

The syndrome of malignant migrating partial seizures in infancy (MMPSI) is one of the most severe epileptic encephalopathies of infancy onset.It has been firstly reported by Coppola in 1995.There have been approximately 80 cases reported worldwide by now,but there is no case reported in our country.The etiology for MMPSI is still unknown,the diagnosis is difficult,and conventional antiepileptic drugs have little efficacy.This paper reviews the possible causes,clinical characteristics,diagnosis and treatment of MMPSI.

Objective To investigate the influence of Wendan decoction on sleep indexes of insomniac patients.Methods 66 patients with insomnia in our hospital were chosen and divided into an experiment group and a control group.The experiment group was treated with the Wendan decoction,and the control group was treated with alprazolam.The curative effects and sleep indexes changes were compared between the two groups.Results The total effective rate of the experimental group was 96.97％ (32/33),which was significantly higher than the control group 66.66％ (22/33) with statistical significance (x2=1 0.1852,P＜0.05).After the treatment,the sleep latency (20.63 ±21.38) min,sleep efficiency (79.73 ± 25.69) ％,total sleep time (6.80± 1.50) h in the experimental group were superior to the control group with sleep latency (65.77 ± 46.41) min,sleep efficiency (61.04 ± 21.76) ％,total sleep time (4.43 ± 2.15) h,with statistical significance (P＜0.05).Conclusion Wendan decoction can prolong sleep time and improve sleep quality for insomniac patients.