Survivin is the strongest member of the inhibitor of apoptosis protein(IAP)family. Smac is the second mitochondria-derived activator of cysteine proteases,which can promote apoptosis by combining with IAP. Abnormal expression of them is closely related with occurrence,development,treatment tolerance and prognosis of ovarian cancer. It is prompted that Survivin and Smac are expected to play important roles in the early diagnosis and targeted therapy of ovarian cancer.

Objective: As the breakthrough for public hospital reform in China, County public hospital comprehensive reform has become the main part of the reform, the reasonable use of New Rural Cooperative Medical System(NCMS) would strongly push the process of new medical reform. Methods: The method of mathematical modeling was applied in policy imitate of hospital operation historical data, to estimate the increasing of decreasing status of NCMS hospital compensation expenditure after the implementation of county-level public hospital comprehensive reform measurement in 10 county-level public hospital. Results: Through analyzing the operation data of applying county-level public hospital comprehensive reform measurement in 10 county-level public hospital from 2010 to 2012, there was 66.67% annual time, the NCMS compensation expenditure for public hospitals increased to 23.075 6 million yuan, which increased by 1.691 7 yuan averagely. Conclusion: After the implementation of comprehensive reform policies, the influencing factors of NCMS funds on the decreasing degree of county-level public hospital expenditure were mainly the proportion of pre drug addition ratio and basic drug sales in the total drug sales.

Objective To study the curative effectiveness of external iliac-popliteal artery artificial graft cross bypass in treating extensive arterial occlusion in unilateral lilac-femoral arteriosclerosis. Methods From Sep. 1999 to Oct. 2007, 39 elder patients were diagnosed as having extensive arterial occlusion in unilateral lilac-femoral arteriosclerosis by color Doppler ultrasonography, CTA or DSA. 25 patients (61.5%) had resting pain, 14 had ulcers or gangrenes in the toes. The average ankle-brachial index was 0. 19. Contralateral external iliac-popliteal artery artificial graft cross bypass surgeries were performed on 22 patients from left to right, the other 17 were from right to left. Results There were no perioperative death or amputation. The ankle-brachial index increased from 0 ～ 0. 41 ( preoperative ) to 0. 85 ～ 1.02(postoperative) ; the claudication distance increased from 15 ～ 60 m (preoperative) to 350 ～ 500 m or more than 500 m [ postoperatively at the speed of (100 ～ 120) m/min] ; The postoperative color Doppler uhrasonography showed the average velocity of the popliteal artery was 45 cm/s. In the anterior tibial artery or the posterior tibial artery, little blood flow was showed in preoperative color Doppler ultrasonography examination, the postoperative average blood flow velocity was 41 cm/s. 35 patients (89. 7% ) were followed up for an average of 3.4 years, with an accumulative patency rate of 85.7%. Four patients had amputation, the limb salvage rate is 88.6%. Conclusion External iliac-popliteal artery artificial graft cross bypass is effective in treating unilateral lilac-femoral arterial occlusion, particularly for aged patients with vital organ incompetence.

Developing along with the chemicals to tend gradually hard and adopting the natural medicine to rise,the international pharmacy field interesting in Chinese materia medica(CMM) increases gradually.The exterior competition of the CMM enterprises is becoming drastic and the secondary deve-(lopment) of CMM for the medicine enterprises in our country should be a better choose to face the international medicine market competition.The internal and external process about the secondary development of CMM should be taken into account and the strategy that should be adopted in the secondary development put forward.Such as: secendary development of new medicine of the CMM compound recipe;turning deeply to the CMM foundation research;the importance of developing the safe and valid CMM whose qua-(lity) is stabile and controlled;the importance of rising the processing level of the product,etc.It emphasized the importance in appling modern science and technology in the text.

Radiotherapy is an important treatment for malignant tumors, but it will have a negative impact on the reproductive function of young female patients.In recent years, the measures to protect female fertility are increasing, including clinical mature technologies such as embryo cryopreservation and mature oocyte cryopreservation; highly experimental technologies such as immature oocyte in vitro fertilization and ovarian tissue cryopreservation and transplantation.In addition, the application of stem cells and the proposal of artificial ovary also show great prospects in the protection of female fertility.This paper reviews the research progress of female fertility protection strategies in radiotherapy.

To elaborate the principal pathogenesis is that imbalance of zang-fu organs and disorder of qi lead to internal wind ,which instigates pathogenic phlegm ,fire and blood stasis to the lesion of brian function.Then to advocate applying Chinese medicine intervening timely the treatment,so as to dispel pathogeny and recuperate the function of zang-fu organs. Moreover to illustrate the concrete clinical adhibition and do some salutary exploration particularly in the aspect of decreasing the number of outbreak.

Objective To investigate the effect of arsenic trioxide (As2O3) and imatinib mesylate (imatinib) or in combination with imatinib with different concentration on Raji cells and the mechanisms.Methods The inhibition of cell proliferation was measured by MTT assay to assess the cells survival after As2O3 and imatinib or in combination with imatinib treatment with different concentration at indicated time.Caspase-3 activity changes and the relative number of cells in different phases and the percentages of cells calculated in G1 and S phase of the cell cycle were assessed by FCM. The expression of p16 protein was analyzed by SABC immunohistochemical method. Results The results of MTT assay showed that As2O3 and imatinib or in combination with imatinib could inhibit Raji cells growth. There was clear statistical significance between the union groups and the single-drug group (P<0.05). As2O3 inducing apoptosis was accompanied by up-regulation and activation of apoptosis protein Caspase-3. The mean percentage of apoptosis cells was both in time-and dosage-dependent form (P<0.0001). While Raji cell lines were less sensitive to imatinib than arsenic trioxide (P >0.05). Further more, Combination of imatinib with As2O3 had not a synergistic inducing apoptosis effect on Raji cells (P >0.05). The optical density of p16 protein increased both in time-and dosage-dependent form with As2O3 by immunohistochemical method (P<0.05). While at higher concentration and for a longer time, imatinib could up-regulated the optical density of p16 protein. There was no obvious statistic significance for p16 protein in Raji cells with using As2O3 only compared with the unions group(P >0.05). The cell cycle was arrested at G1 phase, the number of cells G1 period increased significantly,and S phase decreased on Raji cells after As2O3 treatment. The relationship between the cellular DNA contents and the concentration of As2O3 showed a dose-and time-dependent manner (P<0.0001). But it was found that imatinib had no effect on Raji cell cycle. There was no the obvious difference (P >0.05) between two drugs unions group with only using As2O3 group. Conclusion The results showed that As2O3 exerted variable and definite effects on lymphoma Raji cells, and suggested that As2O3 might induce apoptosis and up-regulate the optical density of p16 protein and arrest cell cycle. As for Raji cells, imatinib might affect the expression of p16 protein at higher concentration. Two drugs unions had no effective and synergistic effect.

Bendamustine hydrochloride is a bifunctional alkylating agent with clinical activity to a number of cancers.Bendamustine,an emerging nitrogen mustards anticancer agent,can improve tolerability without sacrificing clinical activity.Although bendamustine has been used widely for more than 40 years,it was not systematically studied in lymphoproliferative disorders until the 1990s.Bendamustine molecule is more stable than the molecules of other alkylators,and it can cause more extensive and more durable damage to DNA.Unlike other alkylating agents,primary targets of bendamustine are based on excision repair pathways rather than mismatch repair pathways,and it activates DNA damage stress responses,induces apoptosis,inhibits mitotic checkpoints,and induces mitotic catastrophe.This paper will review the role of bendamustine in chronic lymphoblastic leukemia(CLL),indolent non-Hodgkin's lymphoma (NHL),diffuse large B-cell lymphoma (DLBCL),mantle cell lymphoma (MCL),peripheral T-cell lymphoma (PTCL),and Hodgkin' s lymphoma (HL).

BACKGROUND:Anatomical studies on cutaneous nerve around the knee are less in China. Revision surgery or long-term use of medication is commonly used for intractable pain after knee replacement. However, these methods cannot effectively relieve postoperative pain. OBJECTIVE:To explore the therapeutic effect of denervation scheme on intractable pain in patients with knee joint pain after knee replacement through identifying the distribution of the cutaneous skin around the knee joint in the study of anatomy of the knee joint. METHODS:Two adult cadavers and two fresh adult cadavers were dissected to observe the distribution of cutaneous nerves around the knee joint. 100 cases of knee joint of patients with intractable pain after total knee arthroplasty were randomly divided into two groups (n=50). Patients in the observation group underwent denervation scheme. Patients in the control group underwent drug treatment. Patients were followed up after surgery. Bone joint index score was used to evaluate the knee joint function. 36-Item Short-Form Health Survey (SF-36) was utilized to evaluate the quality of life. Visual analogue scale was employed to assess the patient’s pain. Adverse reactions were recorded. RESULTS AND CONCLUSION:(1) Distribution of cutaneous nerves around the knee was fixed. Moreover, each cutaneous nerve had a more definite bony landmark, and could provide anatomic basis for related surgery. (2) Visual analogue scale scores were significantly lower in the observation group than in the control group (P<0.05). (3) Bone joint index score was lower, but SF-36 scores were higher in the observation group than in the control group at 1, 3, 6 and 12 months after surgery (P<0.05). (4) Three cases affected joint swel ing in the observation group and two cases suffered from joint swel ing in the control group. No significant difference in joint swel ing was detected between the two groups (P>0.05). (5) Necroscopy could identify anatomical distribution of cutaneous nerve around the knee. On this basis, denervation therapy can effectively improve symptoms and bone quality in patients with intractable pain, improve the quality of life of patients, and have better safety.

Objective:To establish the best processing conditions of fried with earth for Atractylodes macrocephala.Methods:The contents of water extract , ethanol extract and atractylenolide Ⅲwere used as the evaluation indices , the temperature of pot room , pro-cessing time and the amount of terra flava usta were chosen as the influencing factors , and the processing conditions of fried with earth for Atractylodes macrocephala was optimized by L9(34) orthogonal design.Results:The best conditions of fried with earth processing technology for Atractylodes macrocephala were as follows:the pot temperature of 220℃-250℃, the processing time of 6min and the a-mount of terra flava usta of 30%.Conclusion: The processing technology optimized by orthogonal design can ensure the quality of products .