1.STUDY ON THE ERROR THAT WAS ACCOMPANIED WITH BODY GIRTH
MITSUTSUGU ONO ; MOTOKO KOBAYASHI ; TERUO KAISE ; KAZUKO NAKAGAWA ; TAKAHIRA NOMURA ; MICHIAKI IKEDA ; HILOSHI KURATA ; AKIHITO YANAGIMOTO ; KUNIHIKO HARADA ; TERUYA AMAMIYA
Japanese Journal of Physical Fitness and Sports Medicine 1971;20(3):159-168
10 male and 8 female skillful tester on physical fitness measurements were attended. They were measured one another the body girth and blood pressure of themselves. The result obtained on the same person by the different tester were not always coincident. The following issues were obtained.
(1) Mean value of individual error finding on the systolic pressure was about 4mmHg, on the diastolic pressure was about 6mmHg. Mean value of the difference between the maximum and minimum value of the systolic pressure reported on the same person was 15.4mmHg in male group. That of the diastolic value was 21.4mmHg in male group. For the female group, that of the systolic pressure was 17.0mmHg, that of the diastolic pressure was 23.0mmHg.
(2) Mean value of individual error (as under, be showed by“error”) obtained on the chest girth was 1.43cm in male group, 1.05cm in female group. Mean value of the difference between the maximum and minimum value (as under, be showed by“breadth”) was 5.99cm in male, 4.08cm in female.
(3) “Error”observed on the overarm extended girth was 0.73cm in male, 0.79 cm in female, “breadth”was 2.96cm in male, 2.99cm in female.
(4) “Error”finding on the overarm flexed girth was 0.60cm for both sex, “breadth”was 2.81cm in male, 2.43cm in female.
(5) For the forearm girth, “Error”was 0.40cm in male. 0.24cm in female, “breadth”was 1.81cm in male, was 1.35cm in female.
(6) For the thigh girth, “Error”was 0.90cm in male, 0.69cm in female, “breadth”was 3.91cm in male, 2.93cm in female.
(7) On the calf girth measurement, in male and female group, “Error”was 0.44 and 0.40cm, “breadth”was 1.51 and 1.48cm respectively.
From above mentioned findings, we considered that“Error”is possible to regard as unavoidable error, “breadth”might be probable error.
2.Multiple variant mRNAs with different length tandem repeats of (CAYYCC)n produced from bovine selenoprotein P-like protein gene.
Kiyofumi SAIJOH ; Tatsuya KOBAYASHI ; Motoko NAGANO-FUJII ; Shigeki FUJII ; Hironobu KATSUYAMA
Environmental Health and Preventive Medicine 2005;10(2):103-110
In contrast to selenoprotein Ps (SeIPs) from other animal species, bovine selenoprotein P-like-protein (SeIPLP) was found to contain a tandem repeat of (CAYYCC)(11). During an investigation into whether SeIPLP was a bovine substitute for SeIP or uniquely bovine, its mRNA was found to consist of multiple variants with different length tandem repeat, namely p(0) with (CAYYCC)(11), p(-4) lacking (CAYYCC)(4), p(-8) lacking (CAYYCC)(8), and p(-9) lacking (CAYYCC)(9). Although they were encoded on a single gene locus, neither classicalGT-AG: nor minor classAT-AC: donator-acceptor sequences for alternative splicing were identified. A subsequent S1 protection assay using oligonucleotides, whose sequence may occur as variants, performed against bovine poly(A)(+)RNA identified a total of nine variants. Judging from the sequence of these variants and the branch point mapping, the consensus sequence for recognition of the donator was CACCCCCAC: and of the acceptor and the branch point A nucleotide,ACCCC: CAT orACCCC: CATCCCCAT. Furthermore, when the p(0) insert mRNA was expressed in COS-7 cells derived from an African green monkey kidney, cDNAs corresponding to p(-8) and p(-9) could be isolated. Therefore, the bovine SeIPLP mRNAs consisted of multiple variants probably due to a novel splicing mechanism which was not bovine-specific but common to other mammals.