The platinum-based chemotherapy play an important role in alleviating symptoms of advanced ovarian cancer patients and prolongation of survival time ,The multidrug resistance(MDR),however,seriously affects the chemotherapy affect, the current MDR and its Mechanism studies show that,as a phase Ⅱ detoxification enzymes ghtathione S-transferase-π(GST-π) promote tumor cell resistance to chemotherapoutic drugs by affecting the biotransformation and metabolic processes of drugs,and closely related to MDR in ovarian cancer.

The study on susceptibility genes play an important role in early diagnosis and prevention for the individuals with family history of breast carcinoma,elucidation of pathologic mechanism of sporadic breast carcinoma,early diagnosis and adjudging prognosis,research on drug sensitivity,differential diagnosis of benign and malignant disease,etc.Two major susceptibility genes for breast cancer,BRCA1 and BRCA2,are identified,respectively.Other tumor susceptibility genes such as TP53 are known to increase breast cancer risk of BRCA1 and BRCA2.

Objective To survey the treatment effect of Inhibace on heart failure combined with arrhythmia. Methods Clinical diagnosis of cardiomyopathy 13 cases,carditis 11 cases,hypertensive heart failure 9 cases,cardiac infarction 9 cases,and rheumatice heart failure 2 cases were divided in NHYA Ⅱ 7 cases,Ⅲ 29 cases,Ⅳ 8 cases. There are premature beat in 11 cases,atrial fibrillation in 11 cases, ventricular tachycardia or ventricular fibrillation in 4 cases,and heart conductive block in 13 cases. Orally Inhibace 5 mg qd for two weeks and monitored by clincal synptoms and 24 hours Holter determination. Results The 41 cases of total 44 patients had the obvious effect(75%) and effectiveness(16%) accoording the health ministry heart diseases therapy standard,otherwhile 3 cases had no effectiveness(3/44).Conclusion Inhibace is a kind of ACEI,it ameliorate the circulating volumes,the loading and electrophysiology of heart muscles,that made the good effect in failure heart and decreased the ocurrence of arrhythmia.

Most drugs of amphetamines contain chiral centers, which form different optical isomers, or enantiomers. Because different enantiomers have different pharmcological effect and have different machnism of metabolism. Besides, the ratio of the two eanatiomers could reflect the route and method used in the synthesis of the drugs. So the separation and determination of these eanantiomers for seizured samples or for biological samples became very important in the sence of forensic science.The paper used two chiral reagents: N-trifluroacetylprolyl chloride (TPC) and ( R )-( + )-α-methoxy-α-(trifluormethyl)phenylacetic acid (MTPA) to reach the purpose. They reacted with amphetamine enantiomers to form diasteromeric pairs, which possess some diffeences in physical and chemical natures and could be separated by GC/MS. The paper examined in detail some fectors such as the solvents, chiral reagent amounts, reaction time,temperature,etc. on the effect of chiral derivatization. Some enantiomers of amphetamine (Am),N-methylamphetamine(MAm),3,4-methylenedioxyamphetamin (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3, 4-methylenedioxy-N-ethylamphetamine ( MDEA ) and N-methyl-1-( 3, 4-methylenedioxy )-2-butanamine (MBDB) were well separated each other.

Objective: To study the relationship between negative behavior of preschool children and behavior of their mothers. Method.. 742 mothers of children in their 3 ～ 6 year old age were tested by self- made questionnaire. Results: Negative behavior was not significantly related to age and gender of children. It was negatively related to maternal supporting behavior and positively to maternal un- supporting behavior. The culture background (urban/rural), family income and mother' s occupation were found to be important factors mediating maternal un- supporting behavior. Conclusion: Negative behavior of preschool children is influenced by mother' s behavior, which in turn has its cultural - economical background.

Congenital heart disease(CHD) is one of the most common birth defects,which is caused by either hereditary or environmental factors,and more commonly by the combination of both.Although CHD is known to be a polygenetic disease,its molecular pathogenesis is still poorly understood.Many genes including the transcription factors have recently been identified to be involved in the development of heart as well as in the pathogenesis of CHD.At the molecular level,this paper reviews the roles of the transcription factors of GATA4/5/6,Nkx2.5,Nkx2.7,TBX1,TBX5,and TBX20,and the interactions among them in the abnormal development of the heart.

Food allergy is the earliest and most common allergic disease in infants that affects children' s growth and development.It is closely related to other allergic diseases with a great increase in prevalence.It is important to learn the mechanisms and related factors,investigate the prevention and treatment,and explore the influence to other allergic diseases with food allergy in infants.

ObjectiveTo observe the influence of mini-invasive evacuation of hematoma for myelin basic protein (MBP) and neuron-specific enolase (NSE) in patients with cerebral hemorrhage.Methods Eighty patients with cerebral hemorrhage were divided into group A and group B by random digits table method with 40 cases each.The patients in group A were given conservation treatment.The patients in group B were given mini-invasive evacuation of hematoma treatment.At the same time 30 cases of healthy people randomly were selected as control group.The serum MBP and NSE were determined before treatment,after treatment 1,3,7,14 d in group A and group B.ResultsThe effective rate in group B was 92.5％ (37/40),which was higher than that in group A [80.0％(32/40)],there was significant difference (P ＜ 0.05 ).The serum NSE before treatment in group A and group B was higher than that in control group [ (12.8 ± 2.9),( 13.1 ± 2.8 ) μg/L vs.( 5.3 ± 2.4) μg/L],there was significant difference(P ＜ 0.05 ).The serum NSE after treatment in group B was obviously decreased compared with group A.The serum MBP after treatment 1 d was increased,7 d was decreased,that in group B was obviously decreased compared with group A [ (5.4 ± 1.5) μ g/L vs. (6.9 ± 1.3) μ g/L,P ＜ 0.05 ].ConclusionThe method of mini-invasive evacuation of hematoma in treatment of cerebral hemorrhage patients has good effect,and can decrease the serum MBP and NSE.

Mutations in CD2AP, mapped to 6pL2. 3 and encoding CD2 - associated protein ( CD2AP), are responsible for autosomal recessive or autosomal dominant steroid - resistant nephrotic syndrome (SRNS). CD2AP plays a key role in the slitdiaphragm network of the kidney, which is necessary for the structure and ultrafiltration functions of the slitdiaphragm. The CD2AP homozygous mutation results in early - onset SRNS while heterozygous expression of the CD2AP mutation has increased susceptibility to glomerular injury.No recurrence of proteinuria was observed in the patient with SRNS with CD2AP homozygousmutationafter the renal transplantation. Therefore, detection of the CD2AP gene in the patients suffering from SRNS will be beneficial to making therapeutic decisions and predicting prognoses.

ObjectiveTo explore the relationship between hypoxia induced by cobalt chloride( CoCl2 ) and inflammation in 3T3-LI adipocytes, and the underlying molecular mechanism. Methods3T3-L1 pre-adipocytes were induced under standard differentiation process. Differentiated 3T3-L1 adipocytes were incubated with CoCl2 to induce hypoxia. The mRNA and protein expressions of hypoxia inducible factor-1 α ( HIF-1 α ), glucose transporter 1 ( Glut1 ), adipocytokines, and inflammatory cytokines, as well as the related signaling pathways were determined with quantitative realtime-PCR and Western-blot in this cell model. ResultsCoCl2 at the concentration of 200 μmol/L significantly up-regulated HIF-1 α and Glut1 expressions. After adipocytes were treated with CoCl2, the mRNA levels of endoplasmic reticulum stress marker genes, such as C/EBP homologous protein (CHOP) and glucose-regulated protein78 (GRP78) were markedly increased, adiponectin mRNA expression was significantly decreased, while resistin and leptin mRNA expressions were significantly increased.In addition, the mRNA expressions of cyclooxygenase 2, interleukin-6, and other inflammatory factors were also increased. The phosphorylation of IκBα,which could inhibit the activation of NF-κB, was stimulated by CoCl2.ConclusionsThe results indicate that hypoxia may induce inflammatory response via NF-κB activiation in adipocytes.