Adolescent ; Adult ; Blast Injuries ; complications ; Deafness ; etiology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Treatment Outcome ; Young Adult

Objective To investigate the influence of donepezil hydrochloride and hyperbaric oxygen on cognitive function in patients with vascular dementia.Methods 61 cases of patients with vascular dementia were chosen to research,and were divided into research group(31 cases)and control group(30 cases),the control group was treated with piracetam,the research group was treated with donepezil hydrochloride and hyperbaric oxygen,after 12 weeks of treatment congnitive function,daily life quality and adverse reaction between two group were compared. Results The ADL score and MMSE score between the two group had no significant difference before treatment(all P <0.05).The MMSE score of the research group were higher than the control group[(18.71 ±3.29)points vs (15.83 ±3.45)points]after treatment,the difference was statistically significant (t =3.337,P <0.05).The ADL score of the research group were lower than control group[(37.12 ±7.13)points vs (42.53 ±6.74)points],the difference was statistically significant (t =3.026,P <0.05).4 cases of mild to moderate earache and 1 case of mild diarrhea occurred in the research group,and 1 case of upper abdominal pain occurred in the control group,there was no significant difference in the incidence of adverse reactions between the two groups (χ2 =0.803,P >0.05 ). Conclusion Donepezil hydrochloride combined with hyperbaric oxygen could effectively improve the cognitive function of patients with vascular dementia,and with good safety.

Antigens, CD ; metabolism ; Antigens, Differentiation, Myelomonocytic ; metabolism ; Breast Neoplasms ; metabolism ; pathology ; surgery ; Carcinoma, Lobular ; metabolism ; pathology ; surgery ; Female ; Histiocytoma, Malignant Fibrous ; metabolism ; pathology ; surgery ; Humans ; Immunohistochemistry ; Middle Aged ; Receptors, Progesterone ; metabolism ; Retroperitoneal Neoplasms ; metabolism ; pathology ; surgery

By UV induced mutagenesis of protoplasts of Saccharomyces cerevisiae strain f4, f5 and f6, and screening on plates containing different concertration of ethanol at different temperature, we obtained improved strains, such as f4.2, f5.1, f6.2, f4.5. By using a DES to deal with all the improved strains, We obtained two mutants, f5.1.1 and f4.2.1,which have improved ethanol torlerance. We made use of genome shuffling to generate improved strains in this work. We shuffled twice these improved strains by protoplast fusion and finally obtained strains with higher temperature and ethanol tolerance. we also identified shuffled strains that produced more ethanol by shake-flask experments. At the 35℃, the ethanol yield of R24 strain got to 12.93% (W/V), and were almost 5% higher than that of stain f4.

Objective To study the inhibitory effects of Probucol on the expressions of serum promatrix metalloproteinase-2(pro-MMP-2) and matrix metalloproteinase-2(MMP-2) in patients with coronary heart disease(CHD).Methods The levels of serum Pro-MMP-2 and MMP-2 were detected by zymograph and total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C)were measured by enzyme method in 30 CHD patients before and after treatment with Probucol.Results After treatment of Probucol,the expression levels of pro-MMP-2,MMP-2,TC and LDL-C in serum were decreased than those before treatment,respectively;there were obvious differences of various indexes mentioned above between before and after treatment(P0.05).Conclusion Probucol can inhibit the expressions of pro-MMP-2 and MMP-2 in serum as same as TC and LDL-C.Probucol,as one of TIMPs,may be applied to stabilize plaque..

[Objective] To develop a new type nerve tissue engineering scaffolds to repair the defect of peripheral nerve and observe the growth of bone marrow stromal cells cultured in vitro on the scaffolds.[Method]The biomaterial was made of I-collagen and gelatin by freeze-drying method,the alignment regularities of microscopic channels and there course directions were observed under the scanning electronic microscope.The size of the micropores and the factor of porosity were also measured.The bone marrow stromal cells were seeded on the collagen scaffolds and cultured for 5 days,then the growth of bone marrow stromal cells cultured in the scaffolds was observed.[Result]All the scaffolds were circular cylinder,the microscopic channels were arranged in parallel manners,and the pore sizes of the channels were uniform.The bone marrow stromal cells were seeded in the scaffolds successfully.[Conclusion]The developed nerve tissue engineering scaffolds have good structure and biocompatibility,which can be used in the repair of the nerve injuries.

Objective To investigate the prostecdtive therapeutic efficacy of pulsed radiofrenquency and radiofrequency thermocoagulationof the Gasserian ganglion guided with spiral CT on trigeminal neuralgia. Methods 100 patients with idiopathic trigeminal neuralgiawere treated with pulsed radiofrenquency or radiofrequency thermocoagulation of gasserian ganglion. The numeric rating scales (NRS) ofpain were recorded before and 0.5 year, 1 year, and 2 years after treatment. Results There was no difference in NRS within 1 year, but the recurrencerate was high in the paitents accepted pulsed radiofrenquency 2 years later (P<0.01). Conclusion Pulsed radiofrenquency of thegasserian ganglion is safe and effective on trigeminal neuralgia, but poor in long-term outcome.

Objective To observe the therapeutic effects of stellate ganglion block (SGB) with oxycodone and acetaminophen tablets on the treatment of migraine. Methods 100 patients with migraine were divided into two groups randomly. They received SGB with oxycodone and acetaminophen tablets (experimental group) or only SGB (control group) respectively. The parameters included pain intensity before and 1 d,3 d,1 w,1 m and 3 m after the treatment, and pain relief rate of each patient. The adverse effects were also evaluated. Results The pain intensity described as numeric rating score (NRS) decreased in the two groups at each time point. In the experimental group, the value of NRS was significantly less than that in the control group at 3d,1m and 3m after the treatment. The effec-tive rate ha the experimental group and the control group were 88% and 68%. Conclusion SGB with oxycodone and acetaminophen tablets is more effective for migraine and deserves widely used in patients.

Objective To observe the effect of Sijunzi Decoction (SD) on the mitochondrial DNA deletion of the aging model mice, and to explore its anti-aging biomolecular mechanism. Methods Kunming mice were divided into 6 groups according to the body weight: normal control group, .model control group, high-, middle-and low-dose SD groups (at the dose of 20, 10, 5 g·kg-1 respectively), and vitamin E group (250 mg·kg-1), 20 mice in each group.The mice models of aging were induced by D-galactose. Water maze test was used to evaluate the learning and memory ability of the aging model mice. The effect of SD on the mitochondrial DNA deletion of the aging model mice was ob-served. Results In the hippocampal mtDNA of model control group and low-dose SD group, there were about 3.87 kb fragment missing. But there was no missing pieces in high-and middle-dose SD groups. Conclusion The anti-aging biomolecular mechanism of SD is probably related to the preventive effect on the mutation and deletion of the mitochondrial DNA in the aging model mice.

0.05). But according to the data, IL-8 in Qingqinye low dosage group was lower than model group, positive medicine group, Qingqinye high dosage group and Qingqinye middle dosage group. TNF-? in Qingqinye group was lower than model group and positive medicine group, especially in Qingqinye middle dosage group. Pathological section with articular cavity and surrounding tissues were better in positive medicine group and Qingqinye group. Conclusion Qingqinye has a certain effect for repairing joint immune pathological injury in hyperuricemia model rats.