Objective:To preliminarily discuss the career management of hospital pharmacists from the perspectives of human re-source management and psychology to provide reference for the human resource management of pharmacy department. Methods: The current situation of career management of hospital pharmacists was analyzed based on the theory of Zone of Proximal Development in de-velopmental psychology, and some concepts and measures for the career management of hospital pharmacists were proposed. Results:The managers of pharmacy department must set up dynamic developing view of their employees, establish individualized management concept, transform their role in human resource management and promote the capability of career management. Measures, such as work shift and determined posts, career guidance, potential discovery, opening up promotion ways and self-management of personal career, could be adopted to develop a scientific and effective system of career management. Conclusion:It is suggested that modern manage-ment theories be utilized in the career management of hospital pharmacists, which may help achieve the joint development of department and individuals.

The purpose of this article was to review the current knowledge base related to the risk factors,pathogens,diagnosis and to review and evaluate the strategies to reduce ventilator associated pneumonia (VAP),especially ventilator bundles for prevention of VAP which including elevation of the head of the bed,daily sedative interruption and daily assessment readiness of extubate,peptic ulcer disease prophylaxis,deep venous thrombosis prophylaxis,daily oral care with chlorhexidine.This article also reviewed the role of new type endotracheal tube in reducing incidence of VAP.

Objective:To study the expression of survivin gene in cervical carcinoma and its relation to the pathogenesis of cervical carcinoma.Methods:Using SP immunohistochemical technique,we examined 74 cervical tissues of clinicopathological specimens,including 10 normal cervical tissues,5 cervical intraepithelial meoplasia(CIN)lesions,59 invasive squamous cell carcinomas.Rusults:Expression of survivin were not observed in normal cervical tissues. With the lesions progressing ,the expression rate of survivin protein had a increasing tendency.Expression of survivin was related to the tumor grade and clinical stage.Conclusion:The results suggested that survivin expression is the essential event in carcinogenesis and development of cervical carcinoma.Survivin expression in cervical carcinoma is a useful prognostic marker.

Objective To study the expression of a novel inhibitor gene of apoptosis, survivin, in cervical carcinoma and its relationship with the expression of bcl 2, p53 genes Methods Using streptavidin biotin peroxidase (SP) method, we examined the expression of survivin, bcl 2 and p53 genes in 10 normal cervical tissues and 59 cervical carcinoma tissues Results Survivin was expressed in 41 of 59 (69 5%) cases of cervical carcinomas In contrast, normal cervical epithelium did not express survivin Overexpression of survivin was related to the tumor grade and clinical stage Survivin positive cases were strongly associated with bcl 2 expression (36/45, 80 0% versus 5 of 14 cases, P

By integrating data of sci-tech novelty retrieaval and internet data,the paper makes a precise analysis of the potential demands,interest,interest,tendency,preferences and habits of users of novelty retrieval.From the perspective of big data technology,it analyzes the feasibility of designing targeted and deep-level services for mass user data,puts forward the framework and method of conducting deep-level and precise service recommendation based on users of sci-tech novelty retrieval,and studies the opportunity to provide these users with deep-level and precise services.

Objective To detect the double DNA of trisomy 21 syndrome patients by denaturing high-performance liquid chro-matography(DHPLC) in order to rapidly diagnose trisomy 21 syndrome .Methods To amplify DNA fragments of three short tan-dem repeats of D21S111 ,D21S1411 and D21S1412 using corresponding primers .Then DHPLC was introduced to analyze the DNA fragments in the temperature of 50 ℃ .Results DHPLC elution profiles of D21S111 ,D21S1411 and D21S1412 of normal control showed one peak or two peaks with the same altitude .However DHPLC elution profiles of 21 trisomy syndrome patients showed two peaks of different altitudes ,which one′s altitude was twice than another .Conclusion DHPLC is a sensitive ,convenient ,auto-matic and highly-efficient method to diagnose trisomy 21 syndrome and can be widely used in the clinic diagnosis .

Objective To assess the association between serum γ-glutamyltransferase (γGT) levels and the risk of metabolic syndrome (MS),so as to provide a reference for prevention and diagnosis of MS.Methods Ten papers about the relationship between γGT level and incident MS published in Pubmed,Embase,and the Institute for Scientific Information (ISI),China Academic Journal Full-text Database,China National Knowledge Infrastructure Database and Wan Fang Digital Periodicals Database from January 2000 to April 2014 were retrieved and analysed.Results A total of 65 331 participants from the ten studies were included in the rmeta-analysis.The pooled RR was 2.06 (95％ CI 1.63-2.49) when the incidence of MS was compared between the highest and the lowest groups of γGT levels.A subgroup analysis was made in Asian and western populations.The pooled RR of MS in Asian population was 2.26 (95 ％ CI 1.65-2.87) and in western population it was 1.70 (95 ％ CI 1.15-2.25).Sensitivity analysis confirmed the stability of results.No publication bias was found in our meta-analysis by Egger's and Begg's test.Conclusion Current evidences from prospective studies indicate that risk of metabolic syndrome is increased in correlation with an increase in serum γGT,and serum γGT seems to be an important predictor for MS.

Objective To establish a diagnosis method for fungal infection using two-round PCR,and to evaluate its sensitivity in the detection of clinical specimens suspected to be infected with fungi.Methods A total of 29 specimens of clinical sputum and alveolar wash solution were collected from patients with suspicious fungal infection.All specimens uaderwent direct microscopy with 10%KOH,fungal culture,one-round PCR and two-round PCR.The fungal universal primer targeting ITS regions of rDNA was used in PCR.The detection rate for fungi was compared between these methods.Results The detection rate for fungi was 20.69%by direct microscopy,37.9%by fungal culture,17.2%by one-round PCR,and 48.3%by two-round PCR.More than one species of fungus were detected in 6.9%(2/29),3.4%(1/29)and 24.1%(7/29)of these specimens by fungal culture.one-round PCR and two-round PCR, respectively.There was a significant difference in the detection rate between two-and one-round PCR(x2=6.34,P＜0.05).With regard to the detection rate for more than one species of fungus,two-round PCR was significantly higher than one-round PCR and fungal culture(x2=4.09,6.30.bom P＜0.05).Conclusion Two-round PCR may help to improve the sensitivity of molecular diagnosis of fungus-infected specimens.

OBJECTIVE:To provide reference for the development of pharmaceutical care for periconceptional women. METH-ODS:Probability proportionate to size sampling(PPS)method was adopted to select participants from periconceptional women in Yongchuan District. The participants were investigated by questionnaires about drug use safety knowledge,attitude and behavior. Ep-idata 3.0 software was used to establish database. The data were analyzed with SPSS 15.0 software. RESULTS:Totally 800 ques-tionnaires were distributed and then 779 valid questionnaires were collected with effective rate of 97.38%. The total awareness rate of knowledge about drug use safety was 57.69%. The awareness rate rise with the increase of educational level (P<0.01). The awareness rate was higher in pregnant women than in prepregnant ones,with statistical significance in most of the questions(P<0.05 or P<0.01). For questions about attitude for drug use safety,the positive answer rate was higher in pregnant women than in prepregnant ones,with statistical significance in most of the questions (P<0.01). Among 385 pregnant women,only 56.88% re-ceived folic acid following the WHO recommendation. Of 181 pregnant women who had taken medicine,8.29% indicated that the physicians or pharmacists did not inform that whether the medicine affected pregnancy. CONCLUSIONS:Among periconceptional women,awareness rate of drug use safety is in low level and varied by their educational levels. There are difference in awareness rate of drug use safety and attitude between prepregnant women and pregnant woman. Drug use behavior of pregnant woman is to be modified.

Objective To compare the consistency and difference of optical coherence tomography angiography (OCTA) and traditional multimodal fundus imaging in the diagnosis and activity evaluation of choroidal neovascularization (CNV) in exudative age-related macular degeneration (AMD). Methods A total of 112 exudative AMD patients (130 eyes) were included in this retrospective study, 62 were men (71 eyes) and 50 were women (59 eyes). The mean age was (68.250±9.789) years (range 50–91 years). All patients were underwent traditional multimodal fundus imaging including fundus fluorescein angiography (FFA), indocyanine green angiography (ICGA) and spectral domain optical coherence tomography (OCT);OCTA was performed at the same time. The CNV type was divided into active and non-active according to the results of traditional multimodal fundus imaging. The vascular pattern was divided into active and non-active according to the results of OCTA. Using traditional multimodal fundus imaging as the standard, the sensitivity and specialty of OCTA was evaluated. Results In 130 eyes, CNV was visualized on traditional multimodal fundus imaging in 109 eyes (83.8%);CNV was visualized on OCTA in 103 eyes (79.2%), which including 7 eyes of false negative and 1 eye of false positive. The sensitivity of OCTA for CNV diagnosis was 93.6%, with specificity of 95.2%. The CNV detection rate between two methods had no significant difference (Youden index=0.89, Kappa value=0.796, P=0.07). In 109 eyes diagnosed with CNV by traditional multimodal fundus imaging, 73 eyes (67.0%) were active CNV and 36 eyes (33.0%) were non-active CNV;the vascular pattern was active in 60 eyes (55.0%) and non-active in 49 eyes (45.0%). The sensitivity of OCTA for the detection of active CNV was 82.2%, with specificity of 100.0%. The active CNV detection rate between two methods had no significant difference (Youden index=0.82, Kappa value=0.753, P=0.00). Conclusion In the diagnosis and activity evaluation of CNV in exudative AMD, there is remarkable consistency between OCTA and traditional multimodal fundus imaging.