Objective:To observe and analyze the dynamic changes of hamster's cheek pouch mucosa in different stages of the carcinogenesis under naked eye and light microscope with the carcinogenic agent dimethyl-benzanthracene(DMBA).Methods:DMBA was painted on hamster's cheek pouch mucosa,3 times a week.Dynamic observation of the carcinogenesis of cheek pouch mucous membrane was carried out under naked eye;histological dynamic changes in the microstructure of cheek pouch mucosa in the carcinogenesis were observed under light microscope after the animals were killed respectively in the 2nd week,the 4th week,the 6th week,the 10th week,the 12th week and the 14th week.Results:For the cheek pouch mucosa of hamster painted with DMBA,the simple hyperplasia appeared in the 2nd week,the mild epithelial dysplasia in the 4th week,the moderate dysplasia in the 6th week,the severe dysplasia the partial changes of carcinoma in situ in the 8th week and cancer in the 10th week.All cancer models appearing in the experiment belonged to the well-differentiated squamous cell carcinoma.Conclusion:The carcinoma of hamster cheek pouch mucosa induced by DMBA is similar to the human oral squamous cell carcinoma so it is an ideal model for studying the oral squamous cell carcinoma in multi-stage and multi-step dynamic development.

DNA microarray is the first studied and the most matured form of the bio-chip, which generated huge amounts of data in a number of valuable hidden information. Scientists have attached more importance to the exploration and development for the analysis of these data. At present, many methods have been used for large-scale gene expression data mining, such as statistical analysis, cluster analysis and self-organizing map.This paper reviews the existing methods for analyzing experimental data collected from and introduces some new analytical methods.

Objective To investigate the effects of preoperative anxiety on the recovery of anesthesia and postoperative analgesia in patients undergoing elective laparoscopic ovarian cystectomy.Methods A total of 87 patients were enrolled who were undergoing elective laparoscopic ovarian cystectomy during July 2015 to June 2016.State-Trait Anxiety Inventory-1 was administered to the patients:patients with anxiety were included in the high-anxiety group (Group H) and patients witouth anxiety were included in the low-anxiety group (Group L) We observed the recovery of anesthesia and postoperative analgesia of the patients in two groups.Results In group H,the extubation time,the time for the Aldrete score to reach 9,were significantly longer;more agitation were found;and more fentanyl and parecoxib were needed.The difference was statistically significant (P＜0.05).The age,BMI,operation time,anesthesia time of the patients in the two groups had no statistically significant differences (P＞0.05).Conclusion High anxiety has a negative effect on recovery from anesthesia and on postoperative pain control in patients undergoing elective laparoscopic ovarian cystectomy.

Objective To evaluate the long-term prognostic capacity of the SYNTAX score Ⅱ(SxScore Ⅱ) and SYNTAX score (SxScore) in patients undergoing left main percutaneous coronary intervention (LM-PCI). Methods A total of 209 patients undergoing unprotected LM-PCI in the Cardiology Department of the Affiliated Hospital of PAP of Logistic College were prospectively collected. Follow up was carried out by telephone or outpatient or rehospitalization. The clinical endpoint focused on MACCE after PCI including composite death, nonfatal myocardial infarction, target vessel revascularization and stroke. The secondary endpoint included cardiac death and stent thrombosis. The SxScore and SxScoreⅡ were retrospectively calculated according to results of coronary angiography and clinical features of patients. Patients were stratified according to tertiles of low (≤22), intermediate (23-32), and high (≥33). The clinical data were comparedbetween three groups. The predictive ability of two scoring systems to MACCE after PCI was compared by COX regression evaluation. Results In 209 patients, 12 patients were lost to follow-up (5.7%), and the median follow-up was 30.2 months, 56 cases (28.4%) were observed to suffer from MACCE. The incidence rates of MACCE were 19.0%, 28.6%and 44.4%in SxScore low, intermediate and high groups respectively. The incidence rates of MACCE were 12.8%, 23.8%and 45.5%in SxScoreⅡlow, intermediate and high groups respectively. Single factor analysis showed that SxScore, SxScoreⅡ, age, diabetes and left ventricular ejection fraction (LVEF) were the independent predictors of MACCE. Multivariate analysis showed that SxScore and SxScoreⅡwere still risk independent predictors for MACCE. Conclusion Both SxScore and SxScoreⅡare independent risk predictors for MACCE in patients with unprotected left main coronary artery disease undergoing PCI treatment.

General practice is the core of primary care, and it has become the fundamental part of some high-valued health care system. The function-orientated definition of primary care comprise of five core attributes, which are first-contact, accessibility, continuity, coordination and comprehensiveness of service. This paper started from the definition of general practice and sought to clarify and summarize the definitions, contents and functions of its five core attributes;meanwhile, the high-performance functional mechanism of health care system was also explored. On this basis, four suggestions are put forward: The basic medical health care system should be renamed primary care system;Five core attributes of primary care should be emphasized when strengthening the primary care system; the primary care system should be improved, and the key points are the number of general practitioners (GPs), payment system, primary care purchasing, educational system of GPs and etc.;The management system and service process should be developed which would benefit the implementation of the core attributes of primary care at an organizational level.

Objective To evaluate the incidence and degree of organic impairment after neonatal asphyxia,analyze the high risk factors of this and find a new approach to lower the mortality of asphyxia.Methods Two hundred and twenty-two newborns with asphyxia were studied. They were divided into mild asphyxia group and severe asphyxia group, and the function of main organs were classified as mild and severe, too. Chi-square tests were conducted for statistical analysis.Results The incidences of organic impairment and multi-organ impairment with asphyxia were 90.1 % and 71.6 % respectively. The frequency of organ dysfunction in severe asphyxia was significantly higher than that of mild group(P

Objective To evaluate the parenteral complications and their risk factors and to study the immune status by detecting immunoglobulin and lymphocyte subsets in children with rotavirus enteritis. Methods Sixty-four children with rotavirus enteritis who were treated in Shengjing Hospital of China Medical University between January 2007 and December 2008(observation group) and 18 healthy chiIdren for health screening at pediatric health care center (normal control group) were reeruimd.Clinical manifestations were collected,stool specimens were detected for rotavirus using ELISA,lymphocyte subsets were detected using flow cytometer,and immunoglobulin.liver enzyme and myocardial enzyme were detected.Results Rotavirus enteritis may be complicated by parenteral complications(liver,myocardium,respiratory and nervous system).The activity of ALT,AST,LDH,CK and CK-mB were higher in observation group than that in normal control group(P<0.05).In observation group,liver injury rate in children younger than 12 months was higher than that in children older than 12 months,and the activity of CK and CK-mB were highcr in severe diarrhea cases[CK(324.5±995.5)U／dl,CK-mB(93.8 4±61.5)U／dl]than that in mild diarrhea cases [CK(252.8±130.4)U／dl,CK.mB(59.6±32.6)U／dl](P<0.05).The level of IgG was lower in observation group[(4.46±1.56)g／L]than that in control group[(5.80±1.67)g／L](P<0.05).Lymphocytes subsets study revealed that the activity of CD4+ in observation group[(29.0±4.18)％]was lower than that in control group[(38.6±3.97)％](P<0.05),the activity of CDl9+ [(38.8±3.94)％]was higher than that in control group[(23.1±7.70)％](P<0.05)and the CD4+／CD8+ ratio was reverse in observafion group.Conclusion Rotavirus enteritis may be complicated by parenteral injuries which get liver,myocardium,respiratory and nervous system involved.Children with rotavirus enteritis Call lead to low immune function.Determination of liver enzyme,myocardial enzyme,immunoglobulin and lymphocyte subsets has important clinical significance to monitoring the change of condition and guiding treatment.

Trials in public hospital reforms bear special significance for rational deployment of health resources and exploring the separation of hospital management from routine operations. The paper identified such setbacks in the present trials as easy technology measures, numerous models of separations, difficulty in setting pharmaceutical service fees, and inadequate governance. Authors also recommended such measures as searching for appropriate reforms for public hospitals locally, greater financial resources for completing the compensation mechanism of public hospitals, streamlining their management system for separation of management from operations, and improving governance. All these efforts are designed to enhance the public benefit nature of public hospitals.

Objective To observe the suppressive effect of combination of tissue plasminogen activator(t-PA),heparin and homoharringtonine on the formation of proliferative vitreoretinopathy (PVR) after vitreoretinal surgery. Methods Forty-three cases (44 eyes)of complicated retinal detachment who receivedvitreoretinal surgery were divided into 2 groups.Twenty cases(20 eyes)in group A were treated by intravitreal injection of above mentioned drugs at the end of operation,while no intraocular injection of drugs given in 23cases(24 eyes)in group B.The mean follow-up period was 7.9 months. Result The rate of recurrent PVR in group A was 15.8%(3 of 19),and 45.5%(10 of 22) in group B (P

Objective To study the effect of Tibetan MedicineManu-Xitanggranules on the expression of prostaglandin E2 and TNF-a on adjuvant arthritis (AA) rats.Methods A total of 60 rats were randomly divided into the control group, the model group, the Aspirin group, the low-, medium- and high- dose Manu-Xitang granules groups (each group with 10). Complete Freund's adjuvant method was used for the adjuvant arthritis model except the control group. All the groups started treatment at 8th day, and the treatment last 20 days. The low-, middle- high-dosage groups were treated with 2.0, 1.0, 0.5g/(kg body weight) Manu-Xitang granules, while the the Aspirin group with ASP (0.27 g/kg), control and medol groups with the equal Volume saline. The body weight, the swelling of primary side of arthritis index were observed. The levels of PGE2 and TNF-α were measured by ELISA.Results At 6th, 12th, 18th day, compared with the model group, the body weights in Aspirin group, the low-, middle-, high-dosage groups significantly increased (P<0.05 or P<0.01); at the 6th day, the foot swelling (0.40% ± 0.18%, 0.50% ± 0.25%, 0.55% ± 0.35%vs. 0.85% ± 0.15%) in the Aspirin group, middle-, and high-dosage groups significantly decreased (P<0.05 or P<0.01). And the PGE2 (0.66 ± 0.31vs. 0.39 ± 0.11) and TNF-a (0.72 ± 0.24vs. 0.50 ± 0.15) in the high- dose group were significantly lower than the model group (P<0.05).Conclusions The Tibetan medicineManu-Xitanggranules could treat AA through the changes of PGE2 and TNF-a.