Objective To study the CT manifestations of thyroiditis. Methods CT findings on plain and contrast enhanced thin-slice CT scan of 36 cases with thyroiditis proved by surgery (34/36) or fine needle biopsy (2/36) were analyzed retrospectively.Results There were 28 cases of Hashimotos’ thyroiditis.The characteristic signs were seen in 68% cases (19/28).Plain CT scan showed diffuse and symmetrical enlargement of bilateral leaves and isthmus whith greately reduced density which was homgeneous or slightly heterogeneous,no calcification or lower density nodule was seen,conrast-enhanced CT scan demonstrated uniform enhancement which was more dense than adjacent muscles, the thyroid outline could be clearly defined and no lymphadenopathy was found. Symmetrical enlargement of bilateral leaves and isthmus with uneven density and multiple hyperdens nodules were presented in 1 case. 5 cases of Hashimotos’ thyroiditis associated with multinodular goiters revealed asymmetrical thyroid enlargement and uneven density on plain and conrast enhanced CT images. In 3 cases the thyroid gland was normal in size and shape,except for the reduced ,uneven density. Of these 3 cases,1 showed the enlargement of left lobe with a calcified nodule. There were 8 cases of subacute thyroiditis,the thyroids were asymmetrical enlargement ,strongly decreased in density with obscure margin and slight to intermediate enhancement.Conclusion CT is a very important modality for the diagnosis and differential diagnosis of various thyroidities.

ObjectiveTo investigate the distribution of types of human papillomavirus(HPV)in cervical cancer Xinjiang Uyghur women.MethodsFrom june 2008 to April 2010,patients in the gynecological departmen of people's Hospital of Xinjiang Uygur Autonomous Region,who was gidiagnosed cervical cancer and came from southern of Xinjiang.Cervical cells of these patients were collected,HPV genotyping DNA chip was used to detect HP-DNA of each collected sample.ResultsTotally 120 wommen were screened,the infecton rationof HPV was 95.8%(115/120).The positive rate of HPV-16 infection was 69.6%(80/115);other highrisk HPV infection rates were ranked from high to low as HPV-56,HPV-33,HPV-18,HPV-45,HPV-58,HPV-51,HPV-59,HPV-31,HPV-52,HPV-39,HPV-68,HPV-73,low-risk HPV infection rates were ranked from high to low as HPV-11,HPV-42,HPV-43,HPV-6.However,HPV-35,HPV-53,HPV-66,HPV83,HPV-MM4 and HPV-44 were not detected.Single infection of HPV16 or HPV56 was 53.9%(62/115)and 17.4(20/115)respectively,ultiple infection rate was 29.6%(34/115).ConclusionHPV-16 is the most common types in cervical cancer of Xinjiang Uyghur women.HPV56 maybe susceptible to the Xinjiang Uyghur women,reflecting the specificity of HPV infection in cervical cancer of Xinjiang Uygur women.It needs deep study to development a suitable HPV vaccines.

BACKGROUND:Coronary artery stents can be used in clinical treatment of various lesions due to coronary artery stenosis, and different types of drug-eluting stents and bare metal stents can be used surgicaly. However, there are some differences in the therapeutic efficacy and safety among the stents made in different manufacturers. OBJECTIVE:To explore the safety of biodegradable sirolimus eluting stents from different manufacturers in the treatment of coronary artery stenosis. METHODS:Totaly 193 patients with coronary artery stenosis were enroled, including 116 males and 80 females, aged 37-81 years old. These patients were equaly divided into two groups and respectively treated with Firebird stent (MicroPort) and Partner stent (LOOP INC). Patients were folowed up for 12 months, and the restenosis rate,incidence of acute myocardial infarction, rate of coronary artery bypass graft or secondary percutaneous coronary artery interventional therapy, and mortality rate were compared between two groups. RESULTS AND CONCLUSION:After 12 months of folowed-up, there was no difference in the restenosis rate, incidence of acute myocardial infarction, rate of coronary artery bypass graft or secondary percutaneous coronary artery interventional therapy, and mortality rate between two groups (P> 0.05). During the folow-up, no adverse reaction occurred in both two groups. These findings indicate that different brands of biodegradable sirolimus eluting stents can obtain good outcomes in the treatment of coronary artery stenosis, have no adverse reaction, and exhibit a certain degree of security.

Objective:To investigate the relationship between human papilloma virus (HPV)16 E6/7-specific T cell immune response in the periphral blood and clinical features and prognosis of patients with cervical squamous cell carcinoma (CSCC).Methods:Seventy-two patients pathologically diagnosed with CSCC admitted to Affiliated Tumor Hospital of Xinjiang Medical University from June 2013 to October 2015, and 75 healthy controls were enrolled in this study. The special responses of peripheral blood T cells to E6 and E7 overlapping peptides before treatment were detected by enzyme-linked immunosorbent assay (ELISA). The differences of frequency and intensity expression of specific immune responses between two groups were analyzed by chi-square χ2 test and nonparametric test. The correlation between antigen-specific immune response and T cell subsets was analyzed by Spearman test. Log-rank test and Cox’s regression model were employed for univariate and multivariate prognostic analyses. Results:The frequencies of HPV16 E6-ad E7-specific T cell responses in CSCC patients were significantly higher than those in healthy controls (51.39% vs. 29.33%, P=0.006 and 45.83% vs.25.33%, P=0.009), and the mean intensities were also considerably higher than those in healthy controls (20.00 SFC/10 6vs.10.76 SFC/10 6, P<0.001 and 16.17 SFC/10 6vs.10.72 SFC/10 6, P=0.017). The intensity of HPV16 E6-specific T cell immune response was positively correlated with the CD 4+/CD 8+ ratio in the peripheral blood of CSCC patients ( r=0.279, P=0.018). And a strong correlation was noted between E7-specific T cell immune response intensity and increasing proportion of NK+ cells ( r=0.274, P=0.020). Univariate and multivariate analyses showed that therapeutic mode (radiotherapy vs. concurrent chemoradiotherapy, HR=2.918, 95% CI 1.454-5.854, P=0.003) and E6-specific T cell response (response group vs. no response group, HR=0.491, 95% CI 0.243-0.99, P=0.047) were the independent prognostic factors influencing the clinical prognosis. The 5-year overall survival in patients with HPV16 E6-specific T cell responses was significantly higher than that in the no response group (64% vs.41%, P=0.041). Conclusions:The intensity of HPV16 E6-specific T cell immune response is positively correlated with the CD 4+/CD 8+ ratio. No HPV16 E6-specific T cell response and radiotherapy alone are more likely to cause poor prognosis of CSCC patients.

BACKGROUND:As a novel noninvasive method, high frequency ultrasound has been used for the detection of skeletal muscle widely. OBJECTIVE:To detect the thickness of the dorsalLisfrancligament between the first (medial) cuneiform (C1) and second metatarsal (M2) using high frequency ultrasound to evaluate the feasibility and clinical application value of high frequency ultrasound for the detection of theLisfrancjoint. METHODS:Both feet of 100 healthy volunteers were included and the thickness of dorsalLisfranc ligament and the distance between C1 and M2wereexamined using high frequency ultrasound. RESULTS AND CONCLUSION:The display rate of high frequency ultrasound for the dorsalLisfranc ligament in the 100 healthy volunteers was 100%. There was no significant difference in the thickness of the dorsalLisfrancligament between both feet or sexes in adults(P> 0.05); as did the distance between C1 and M2 (P> 0.05). These findings suggest that visual dorsalLisfrancligament is detected by high frequency ultrasound. The measurement of the distance between C1 and M2by high frequency ultrasound may be a feasible, noninvasive, low-cost, and early diagnostic method forLisfrancinjuries.

BACKGROUND:Ultrasonography as a noninvasive means has been widely recognized in the examination of the skeletal muscle system. OBJECTIVE: To investigate the clinical significance of ultrasonography for the elderly with degenerative knee cartilage. METHODS:Totaly 102 patients (204 knees) who were≥ 60 years old and successively admitted for knee disease were selected and according to clinical diagnostic criteria of degenerative knee disease, divided into two groups: osteoarthritis group (167 knees) and non-osteoarthritis group (37 knees). Al the knees were subjected to ultrasonography for observing the effusion depth of the suprapatelar bursa, medial bursa and lateral bursa, as wel as the synovium, pannus and cartilage. RESULTS AND CONCLUSION: Of the 204 knee joints, degenerative changes of the knee occurred in 167 knees, primary knee arthritis in 91 knees, secondary knee arthritis in 76 knees, and the degeneration rate was 82%. The volume of effusion in the suprapatelar bursa, medial bursa and lateral bursa was significantly higher in the osteoarthritis group than the non-osteoarthritis group. In the osteoarthritis group, synovial hyperplasia was in 59 knees, pannus formation in 23 knees, rough cartilage surface in 167 knees, and the knee cartilage thinning or loss in 117 knees. In the non-osteoarthritis group, synovial hyperplasia was in 5 knees, rough cartilage surface in 27 knees, and the knee cartilage thinning or loss in 4 knees. These findings indicate that ultrasonography is of certain clinical significance in evaluation of degenerative knee cartilage in the elderly.

Objective:Abnormal Savda syndrome transplantation tumor model was established on the basis ofabnormal Savda syndrome model in order to study the relevance between the abnormal Savda syndrome tumor and the disorder ofthe neuroendocrine-immune network by analyzing morphological and ultrastructural changes ofthe hypothalamus-pituitary-adrenal axis(HPAA).Methods:120 ICR mice were divided randomly into four groups(control, tumor model, abnormal Savda syndrome model, and abnormal Savda syndrome tumor transplantation model).The structural changes in hypothalamus, pituitary and adrenal glands were analyzed by bright-field and electron microscopy.Results:First, in abnormal Savda syndrome, the rate oftumor transplantation was increased significantly than in the normal state(93.3% and 56.7%, respectively, P

Objective To construct eukaryotic expressing vector of mouse soluble CD160 and stably transfect into CHO cells for eukaryotic expression.Methods Recombinant soluble CD160(rsCD160) was constructed by gene recombination.Total RNA was extracted from the spleen of C57BL/6 mice.cDNA was amplified for the soluble form of CD160.Then,the PCR product was cloned tO pcDNA3.1 and pEGFP-N1.The recombinant plasmid was identified by restriction map and sequence analy-sis.The soluble CDl 60 expression in CHO cells transfected with recombinant psCDl 60 was verified by RT-PCR and Westernblot.The binding ability of psCD160 tO its ligand was detected by FACS.Results 520 bp mouse soluble CD160 gene was obtained.Recombinant mouse psCD160 was successfully constructed.After transfection,soluble CD160 expression in the culture supernatant of CHO cells was successfully detected.FACS analysis indicated that soluble CD160 could bind tO its ligand.Conclusion Recombinant mouse psCD160 is successfully constructed,which will benefit our further study on soluble CD160 for immune therapy against tumor in the future experiments.

OBJECTIVETo investigate the methylation levels of death-associated protein kinase (DAPK) in Uyghur female patients with different cervical lesions in Xinjiang, and to discuss the relationship of the expression and significance of DAPK in normal cervix, chronic cervicitis, cervical intraepithelial neoplasia (CINI, CIN II/III) and invasive cervical squamous cell carcinoma.

METHODS30 cases of normal cervix and chronic cervicitis, 30 cases of CINI, 30 cases of CINII/III and 30 cases of cervical squamous cell carcinoma were tested by methylation specific PCR (MSP). Expressions of DAPK in 30 cases of normal cervix and chronic cervicitis, 30 cases of CINI, 30 cases of CINII/III and 30 cases of cervical squamous cell carcinoma were assayed using immunohistochemical SP staining.

RESULTSThe methylation rate of DAPK gene in normal cervix and chronic cervicitis was 3.33%, 10% in cervical intraepithelial neoplasia CINI, 36.7% in CINII/III, and 63.3% in invasive cervical squamous cell carcinoma. The methylation rate of DAPK in the SCC group was significantly higher than that in the other groups (P < 0.05). Aberrant promoter methylation of the DAPK gene was positively correlated with the degree of cervical lesions. The positive rate of DAPK protein in normal cervix and chronic cervicitis was 93.3%, 83.3% in cervical intraepithelial neoplasia CINI, 60.0% in CINII/III, and 33.3% in invasive cervical squamous cell carcinoma. The expression of DAPK in the SCC group was significantly lower than that in the other groups (P < 0.05). The positive rate of DAPK protein was negatively correlated with the degree of cervical lesions (r(s) = -0.603, P < 0.001).

CONCLUSIONSMethylation of DAPK is involved in the cervical carcinogenesis and DAPK gene promoter methylation occurs in the early development of cervical cancer in Uyghur women in Xinjiang. Detection of DAPK gene methylation may provide a basis for use in early detection of cervical cancer. DAPK protein expression is decreasing even disappears along with the progression of cervical lesions.

Adult ; Aged ; Apoptosis Regulatory Proteins ; genetics ; metabolism ; Calcium-Calmodulin-Dependent Protein Kinases ; genetics ; metabolism ; Carcinoma, Squamous Cell ; genetics ; metabolism ; pathology ; Cervical Intraepithelial Neoplasia ; genetics ; metabolism ; pathology ; China ; ethnology ; DNA Methylation ; Death-Associated Protein Kinases ; Disease Progression ; Female ; Humans ; Middle Aged ; Promoter Regions, Genetic ; genetics ; Uterine Cervical Neoplasms ; genetics ; metabolism ; pathology ; Uterine Cervicitis ; genetics ; metabolism ; pathology

Human papilloma virus (HPV) is believed to be an essential factor for the development of cervical cancer. Early diagnosis and treatment of cervical intraepithelial neoplasia can effectively inhibit the future progression. HPV late 1 protein possesses epitope that can identify and adhere to host cells, and thus may play an important role in HPV infection and cervical carcinogenesis.
Capsid Proteins ; Cervix Uteri ; metabolism ; virology ; Female ; Humans ; Oncogene Proteins, Viral ; Papillomavirus Infections ; complications ; Uterine Cervical Neoplasms ; virology