The purpose of this investigation was to determine the effects of daily walking on aerobic work capacity, vital age and other fitness components in middle-aged and elderly walkers. The subjects were thirty-six walkers (average 65.0±8.0 yr, 51-80 yr), whose training period averaged 13.5±9.0 yr. The walkers were significantly greater in maximal oxygen uptake (VO2max), and oxygen uptake at the lactate threshold (VO2LT), and were 6.5 yr younger in vital age (VA) than sedentary middle-aged and elderly. These findings suggest that middle-aged and elderly walkers have more favorable profile of aerobic work capacity and of health status when compared to sedentary persons.