Objective To evaluate the effect of catheterization on the serum prostate specific antigen (PSA) in acute urinary retention (AUR) patients due to benign prostate hyperplasia (BPH). Methods The data of 35 patients with AUR due to BPH were analyzed, and the changes in serum PSA values were compared before and after catheterization. The effect of AUR on serum PSA in BPH patients was discussed. Results It has been found that serum PSA declined from 9.93?8.85ng/ml to 6.46?5.75ng/ml after catheterization for the patients of AUR with BPH, and the values showed a significant difference (P0.05) in the patients in whom the serum PSA ≤4ng/ml, while a significant difference existed in both the group of patients in whom the serum PSA was 4~10ng/ml (from 5.95?1.43ng/ml to 4.09?2.00ng/ml, P10ng/ml (from 18.93?8.49ng/ml to 11.49?6.37ng/ml, P4ng/ml.

Objective To establish a model of homogenous bladder transplantation without blood vessel anastomosis in rat,and to investigate the outcome of the transplanted grafts.Methods 20 newborn SD rats were used as donors,and a total of 20 Wistar rats of 5-week-old were used as the reeipiemts for transplantation of homogenous bladder.The grafts were harvested from the SD rats,and they were wrapped with the greater omentum of the recipients without blood vessel anastomosis.Immunosuppressive agent was given postoperatively.10 rats were sacrificed on the 7th day and the remainings rats were sacrificed on the 14th day after bladder transplantation.The transplanted bladder grafts were observed grossly and microscupicully.Results Of 20 rats undergone homogenous bladder transplantation,19 rats were alive and the grafts were in good condition microscopically.There was no obvious rejection reaction in the transplanted bladders.The bladder mucosa,blood vessels and muscle layers of the grafts appeared to be normal microscopically.Conclusions The homogenous bladder transplantation into the omentum without blood vessel anastomosis in rats is considered to be a kind of stable animal model,with a higher survival rate of the tramsplanted bladder.

Objective To evaluate the long-term complications of continent ileocolic reservoir with in situ appendix conduit through umbilicus and their management.Methods We reviewed 37 patients with bladder diseases who underwent the surgery of continent ileocolic diversion with in situ appendix conduit through umbilicus and followed up for 85 to 136 months(mean 109 months).Long-term postoperative complications were documented.Results Complications occurred in 15 cases(40.5%),including stone formation in urinary pouch in 2 cases,efferent limb stricture and difficulty in catheterization in 3 cases,ureter stricture and hydronephrosis in 7 cases,foreign matter in urinary pouch in 2 cases,efferent limb mucosa exstrophy in 1 case.These complications were correctly treated and good results were achieved.Conclusion Long-term complications of continent ileocolic diversion with in situ appendix conduit through umbilicus were related to nursing in daily life after operation and unsuited design in operation.Nursing in daily life is considered very important in preventing postoperative complications.

Objective To determine the causative association between a newly isolated coronavirus and the current epidemic of severe acute respiratory syndrome (SARS) Methods Coronavirus was isolated from the samples of patients with SARS by cell culture Immunofluorescence assay and neutralization test were used to detect the antibodies in serum of SARS patients against newly isolated coronavirus, in order to analyze and determine the association between coronavirus and SARS pathogen Results The antibodies against the novel coronavirus could be detected in 99 of 113 sera from clinically diagnosed SARS patients The results of 10 pairs of double serum detection showed that, antibody titers in the late phase were significantly higher than those in the acute phase, and a highest increase by 128 folds was being found. The neutralization test showed that the antibodies from SARS patients' sera could neutralize the novel coronavirus Conclusion The newly isolated coronavirus was closely associated with and possibly the key pathogen of SARS

Drug addiction is a chronic and relapsing brain disease, which causes damage to the health of addicts seriously, and causes huge social problems. Relapse is one of the major characteristics of drug addiction, and is the main problem to be solved. In the past several decades, the mechanisms, high relapse rates, abstinence of addiction were studied. The Results of these research findings are summarized in this review to provide a better overview of the mechanisms and treatments of addition in order to provide ideas for further research.

10.3969/j.issn.2095-4344.2013.26.008

Objective To analyze the clinical data of recipients over 15 years after renal transplantation,and to find the factors that affect the long-term survival of recipients after renal transplantation.Method Before June 30,2000,326 renal transplant recipients in our hospital were collected retrospectively.The risk factors which affect the survival of kidney transplant recipients and kidney were analyzed from four dimensions.A Cox model was established to analyze these multi factors.Result Cox hazard model indicated that advanced age (P=0.010,RR =1.052),AMR (P＜0.001,RR =18.311),nonadherence (P =0.001,RR =2.854),smoking (P =0.025,RR =2.097)were the risk factors for recipients' survival.Using immunosuppressive regimen FK506 + MMF+ Pred (P =0.019,RR =0.433),or CsA + MMF + Pred (P =0.019,RR =0.413) was the protective factor for recipients' survival.Nonadherence (P＜0.001,RR =5.645),and diabetes (P＜0.001,RR =3.310) were the risk factors of grafts' survival.Using immunosuppressive regimen FK506 + MMF + Pred (P＜0.001,RR =0.236),or CsA + MMF + Pred (P =0.002,RR =0.317) was the protective factor of grafts' survival.Conclusion To enhance the long-term outcome of recipients and grafts,the individualization of immunosuppressive regiments and controlling of the chronic diseases progress by changing the unhealthy life style are cutting on edge.

Objective:To evaluate the effects of maxillomandibular advancement and contrarotation in the treatment of severe com-plicated multilevel obstructive sleep apnea/hypopnea syndrome (OSAHS).Methods:1 2 patients (all males,aged 26 to 56 years) with severe complicated multilevel OSAHS were treated by orthognathic surgery methods of maxillomandibular advancement and cont-rarotation and followed up for 6 -1 2 months.Results:After surgery the symptoms of all patients,such as snoring,waking up be-cause of stoke and streaming with sweat,disappeared or were ameliorated remarkably.The AHI index declined from 51 -70 before operation to 0 -3 after operation.While sleeping,their blood oxygen saturation augmented from 30% -70.1 % presurgically to 93%-99% postsurgically,and their average blood oxygen saturation increased from 60% -72% presurgically to 95% -99% postsurgi-cally in all 1 2 patients.Conclusion:Orthognathic surgery method of maxillomandibular advancement and contrarotation is effective in the treatment of severe complicated multilevel OSAHS.

Objective To investigate effects of Kanglaite injection on proliferation, cycle and apoptosis of human breast cancer MCF-7 cells;To discuss its relevant mechanism. Methods Logarithmic growth phase cells were divided into control group and Kanglaite-treatment group (10, 20, 40μL/mL). Cells were cultured in RPMI-1640 for 24 h before drug treatment. The inhibition rate of Kanglaite injection on proliferation of human breast cancer MCF-7 cells was detected by MTT assay. Apoptosis and cell cycle of MCF-7 cells were detected by flow cytometry. Changes in cell nucleus were determined by Hochest staining assay. Protein expressions of Bcl-2 and Bax were detected by ELISA and Western blot. Results Kanglaite injection for 12 h, 24 h or 48 h resulted in a significant inhibition of MCF-7 cells proliferation (P<0.05, P<0.01);Compared with the control group, Kanglaite injection-treated cells showed increased percentage in G2/M and G0/G1 phases (P<0.001, P<0.01), but showed decreased percentage in S phase (P<0.01), and apoptosis rate increased (P<0.05, P<0.001). Kanglaite injection significantly decreased protein expression of Bcl-2, and enhanced protein expression of Bax of MCF-7 cells (P<0.01, P<0.001). Conclusion Kanglaite injection can inhibit the proliferation of human breast cancer MCF-7 cells, decrease cell cycle and induce apoptosis, the mechanism is related with decreasing protein expression of Bcl-2 and enhance the protein expression of Bax.

Objective To explore the feasibility of using clinical whole body MR scanners to investigate the intravital visibility of central nervous system (CNS) lesions in rats of multiple sclerosis (MS). Methods Ten Lewis rats were injected with the peptide 35-55 of myelin oligodendrocyte glycoprotein to make the model of MS. On a Siemens Sonata 1.5T MR scanner equipped with a flexible surface coil, rats brain and spinal cord were examined using T2-weighted and T1-weighted imaging with slice thickness of 1-2 mm. On a Siemens Trio Tim 3.0T MR-scanner equipped with a quadrature wrist coil, rats were examined using T2WI, T1WI and Gd-DTPA enhanced T1WI 3-dimensional imaging with voxel size up to 0.06-0.08 mm~3. Rat brain and spinal cord images in multiple orientations were reconstituted with special software in workstation. Results T2WI and T1WI of the lesions in MS rat brain with high spatial and contrast resolution could be obtained with clinical 3.0T MR scanner, though the image resolution of spinal cord was relatively low. The resolution of 1.5T MR was lower than that of 3.0T. Plaques in CNS of MS rats presented as hyperintense areas on T2WI and hypointense areas on T1WI. Contrast enhancement was observed as hyperintense on T1WI. Conclusion High quality images of CNS lesions canbe obtained with clinical 3.0T MR-scanner in MS rat, which offers a noninvasive access for studying CNS diseases in the rats.