DNA fragments of ? subunit and ? subunit were amplified by PCR from the genome of Acidianus tengchongensis with the degenerated primers designed from the consensus amino acid sequence of Sulfolobus chaperonins. The two DNA fragments about 500bp were used as probe for southern hybridization to determine the suitable restriction endonuclease. Restriction endonuclease digested genomic DNA was circularized by self-ligation, and complete sequences of ? subunit and ? subunit were obtained by inverse PCR. The primers oriented in the reversed direction of the usual orientation to amplify the DNA sequence that flank the known region. The complete genes of ? subunit and ? subunit were PCR amplified from genomic DNA using two pairs primers.

Objective To discuss MRI scan in the diagnosis and clinic-pathology of ovarian cystoid-material lesion.Methods We retrospectively analyzed 41 patients with clinic pathologically-proved in 2005~2007,and analyzed US,MR scan and Gd-DTPA in the diagnosis of ovarian cystoid-material lesion.Results The clinical date of the 87 patients were completed.After operation,41 of them were confirmed by pathology to be cystiform or parenchymal process,with age 16~76,average 56,of which,21 cases,were tumour-like lesions occupying 51.2%,18 cases were timorous lesions,occupying 30.2%,13 cases weer ovarian cystadenoma,occupying 30.2%,and 2case ueve other disense,occupying 4.65%.Conclusion The benign often present as ovarian cystoid-material lesion.MRI scan can show the inner structure of ovary lesion.

With the population ageing, the number of elderly patients with end-stage renal disease(ESRD)is increasing, and the proportion of elderly patients undergoing hemodialysis is also increasing year by year.The elderly patients undergoing hemodialysis are susceptible to frailty due to the comorbidities, polypharmacy, chronic inflammation, malnutrition and other factors.In addition, frailty can lead to a variety of adverse prognosis such as falls, fractures, cognitive decline, all-cause hospital readmissions and all-cause death in elderly patients undergoing hemodialysis.

目的探讨校园意外伤害发生的原因、特征和危险因素,为伤害预防与控制、残疾补偿、社会康复的有机结合提供参考依据。方法以1990—2002年因校园意外伤害造成脊髓损伤的 26例残疾学生作为个案调查研究对象。为了解校园意外伤害事故的致残原因及受伤后的处理情况,对仍在院接受治疗的残疾学生进行随访。结果伤害致残者中男生多于女生;初高中学生是伤害致残的高发人群(69%);校园意外伤害致残原因依次为:①体育训练;②跳水、潜水;③嬉闹摔伤;④高处坠落;⑤坠落物撞击;⑥钝器、刀砍击伤。医疗费用73%由校方支付。结论校园不安全隐患是危害学生生命安全的主要危险因素;提高青少年自我保护意识和自我防卫能力、加强安全教育、建立环境安全检查制度、定期对学校设施进行检查和维护是减少校园意外伤害事件的重要措施

Arteriosclerosis Obliterans ; surgery ; Humans ; Lower Extremity

AIM: To determine the effect of phacoemulsification and intraocular lens (IOL) implantation for angle closure glaucoma previously treated by laser iridotomy.METHODS: Thirty-nine patients(39 eyes )with angle closure glaucoma previously treated by laser iridotomy and cataract were divided into two groups according need for anti-glaucoma medications for controlling intraocular pressure (IOP) or not. All the patients underwent phacoemulsification and foldable IOL implantation and were followed up 3 months postoperatively.RESULTS: The best-corrected visual acuity(BCVA) of both groups improved significantly (P<0.05). The post-operative IOP of group I at 3 months was 15.72±3.02mmHg,and that of group Ⅱwas 16.30±3.81mmHg. The IOP of both group decreased significantly(P<0.05). The central anterior chamber depth of group I was deepened from 1.64±0.45mm preoperatively to 3.21±0.41mm at 3 months postoperatively,group Ⅱ was deepened from 1.92±0.52mm preoperatively to 3.18±0.39mm at 3 months postoperatively. The postoperative central anterior chamber depth of both groups increased significantly(P<0.05).Anterior chamber angle was much wider postoperatively.CONCLUSION: Phacoemulsification and IOL implantation was effective for angle closure glaucoma previously treated by laser iridotomy,and can improve visual acuity and completely relieve the pupillary block of angle closure glaucoma.

The recombinant protein drug usually has a short half-life after intravenous (IV) or subcutaneous (SC) administration. The methods of prolonging the half-life of recombinant protein drug in common use are mainly based on three principles: 1 Amplifying the molecule weight of protein drug; 2 Making use of drug balance in the blood; 3 Reducing Immunogenicity. Three methods were focused on: Analog construction, PEGylation and Albumin fusion technology. The characteristics, half-life and immunogenicity problem of their products in the market and under development are summarized.

Hepatitis B is a serious infectious disease worldwide, and hepatitis B virus (HBV) is the direct cause of this disease. In recent years, as an essential part of its evolutionary process, HBV mutation has been extensively studied domestically and globally. However, the study on the conserved sequences in HBV sequences is still in its infancy. In this study, we applied multiple EM for motif elicitation (MEME) algorithm to discover HBV motif and proposed a new metric, conservative index (CI), to carry out phylogenetic analysis based on HBV sequences. Then, the constructed phylogenetic tree was subjected to reliability assessment. The results demonstrated that the new metric CI combined with the MEME algorithm can effectively help to discover motifs in HBV sequences and construct a phylogenetic tree based on them and to analyze the evolutionary relationship between HBV sequences; in addition, the possible ancestral sequences of samples may be obtained by conservative analysis. The proposed method is valuable for the exploratory study on large HBV sequence data sets.
Computational Biology ; Conserved Sequence ; Evolution, Molecular ; Hepatitis B virus ; genetics ; Humans ; Nucleotide Motifs ; Phylogeny ; Reproducibility of Results

Objective To study the electrophysiological properties of rat skeletal muscle within the frequency from 100Hz to 100MHz.Methods The AC impedance of rat sural muscle tissues was measured using the Agilent 4294A impedance analyzer, then the electrophysiological properties of the normal rat sural muscle cells were established in the respect of cell dielectric spectroscopy, Cole-Cole plots, spectrum of loss factor and loss tangent.Results (1) The dielectric response of rat sural muscle cells was frequency-dependent, such as increase in frequency caused decrease in permittivity. At the same time the conductivity trended to arise; (2) The dielectric parameters(?_L,?_h,?_L,?_h,△?″_ max,△tg?_ max,f_ C1,f_ C2) cell fiber parallel to the applied electric field differed from that cell fiber perpendicular to the applied electric field; (3) The effect of alternating current electric field on rat sural muscle cells showed a dielectric dispersion phenomenon, representing two characteristic frequency: the 1 st frequency(f_ C1(∥)=f_ C1(⊥)=1.02 kHz) and 2 nd frequency(f_ C2(∥)=59.31 kHz,f_ C2(⊥)=380.04kHz). Conclusion The method for studying the electrophysiological properties of rat skeletal muscle cells was established in the respect of the frequency domain,providing a possibility for investigating muscle fatigue in the future.

Objective:To regulate operation requirement for electric instrument using in surgery and eliminate hidden danger so as to ensure their safety in using.Methods: Through discussed the regulation of operative process for electric instrument using in surgery to develop a insulation detector which can adapt the particular case of hospital, and then to carry out relevant management for this kind of electric instrument.Results: The simplest insulation monitoring method got best reliable data, and it provide accuracy basis for clinical electric instrument. Therefore, it ensured the safety of clinical application of electric instrument.Conclusion: The special insulation monitor detects electric instrument in surgery has series of merits including low investment, fast detection and reliable performance, it not only can ensure the safety of surgery but also can provide traceable basis for preventing relevant medical dispute.