Objective:To study the effect ofRNA interferenc(RNA)ion FAK gene expression and the biological characteristics of human breast cancer MCF-7.Methods:The constructed recombinant plasmid FAK-siRNA was transfected into human breast cancer MCF-7 cell using LipofectamineTM 2000.The transfection efficiency was observed under fluorescence confocal microscope.Expression of FAK mRNA and protein were detected by reverse transcription polymerase chain reaction(RT-PCR)and Western blot.Distribution of cell cycle was assessed by flow cytometry.Cell penetrate matrigel capacity were determined by invitro experiment.Results:FAK-siRNA effectively inhibited FAK mRNA and protein expression(P

Objective To study the curative effectiveness of external iliac-popliteal artery artificial graft cross bypass in treating extensive arterial occlusion in unilateral lilac-femoral arteriosclerosis. Methods From Sep. 1999 to Oct. 2007, 39 elder patients were diagnosed as having extensive arterial occlusion in unilateral lilac-femoral arteriosclerosis by color Doppler ultrasonography, CTA or DSA. 25 patients (61.5%) had resting pain, 14 had ulcers or gangrenes in the toes. The average ankle-brachial index was 0. 19. Contralateral external iliac-popliteal artery artificial graft cross bypass surgeries were performed on 22 patients from left to right, the other 17 were from right to left. Results There were no perioperative death or amputation. The ankle-brachial index increased from 0 ～ 0. 41 ( preoperative ) to 0. 85 ～ 1.02(postoperative) ; the claudication distance increased from 15 ～ 60 m (preoperative) to 350 ～ 500 m or more than 500 m [ postoperatively at the speed of (100 ～ 120) m/min] ; The postoperative color Doppler uhrasonography showed the average velocity of the popliteal artery was 45 cm/s. In the anterior tibial artery or the posterior tibial artery, little blood flow was showed in preoperative color Doppler ultrasonography examination, the postoperative average blood flow velocity was 41 cm/s. 35 patients (89. 7% ) were followed up for an average of 3.4 years, with an accumulative patency rate of 85.7%. Four patients had amputation, the limb salvage rate is 88.6%. Conclusion External iliac-popliteal artery artificial graft cross bypass is effective in treating unilateral lilac-femoral arterial occlusion, particularly for aged patients with vital organ incompetence.

Developing along with the chemicals to tend gradually hard and adopting the natural medicine to rise,the international pharmacy field interesting in Chinese materia medica(CMM) increases gradually.The exterior competition of the CMM enterprises is becoming drastic and the secondary deve-(lopment) of CMM for the medicine enterprises in our country should be a better choose to face the international medicine market competition.The internal and external process about the secondary development of CMM should be taken into account and the strategy that should be adopted in the secondary development put forward.Such as: secendary development of new medicine of the CMM compound recipe;turning deeply to the CMM foundation research;the importance of developing the safe and valid CMM whose qua-(lity) is stabile and controlled;the importance of rising the processing level of the product,etc.It emphasized the importance in appling modern science and technology in the text.

Objective To investigate the correlation between the expression of p170、GST-π 、TOPO Ⅱ and the expression of Her-2 in human breast cancer. Methods The expression of Her-2 was detected by fluorescence in situ hybridization.The expression of p170,GST-π,TOPO Ⅱ were tested in 48 breast cancer by immunohistochemical SP method,and their correlations with clinicopathological features and the expression of Her-2 were analyzed.Results The positive rate of p170,GST-π,TOPO Ⅱ were 43.8 ％(21/48),39.6 ％(19/48),56.3 ％(27/48),respectively. The expression of p170, GST-π,TOPO Ⅱ positively correlated with the expression of Her-2(P＜ 0.05).The levels of p170,GST-π,TOPO Ⅱ expression were not associated with age,primary tumor and lymph node metastasis(P ＞ 0.05).Conclusion There is great possibility of chemotherapy drug resistance in breast cancer with expression of Her-2.Expressions of p170,GST-π,TOPO Ⅱ and Her-2 have an instructive significance for chemotherapy.

As a new biotechnology, protein transduction connects the target fragment with biology active and protein transduction domain, and transducer it in to cells, exert its biological effects. Protein transduc-tion domain is a small molecule peptide, not only the protein transduction domain can penetrate through cell membrane, but could maintain the activity of its fusion protein. Protein transduction is an emerging biology technology, it has been applied into all aspects of cell gene regulation since its invention. In this article, we take Tat, Pdx-1 and Beta/NeuroD for instance and review the application of transduction protein in the dif-ferentiation of pancreatic stem cell.

Thyroid cancer accounts for the most common endocrine malignancies in women during childbearing age.Many questions remain to be answered regarding the association between thyroid cancer and pregnancy.This review will discuss both the effects of pregnancy on thyroid cancer and vice versa.Recommendations on how to manage thyroid cancer in pregnancy will also be cited from a Chinese academic guideline as listed at the end of this review.

Liver cancer is one of the most common cancers worldwide and the third leading cause of cancer death.Partial hepatectomy and liver transplantation are the most effective therapies.However, postoperative tumor metastasis and recurrence are the main obstacles in the long-term survival.Liver cancer stem cells (LCSCs) within cancer tissues are associated with tumor occurrence, proliferation and tolerance to current therapy and are regarded as the major root of metastasis and recurrence.Eradication of LCSCs is a novel therapy of liver cancer.In this review, surface markers of LCSCs and mechanisms of pro-metastasis and recurrence, circulating LCSCs,microenvironment of LCSCs and their roles in the metastasis and relapse are summarized.

Papillary carcinoma is one of the commonest tumors in the thyroid.The pathologic diagnosis of papillary carcinoma as well as follicular carcinoma and follicular adenoma of the thyroid are based on the morphology observation hitherto.However,the diagnostic value of immunostaining for distinction among differentiated neoplasm of the thyroid was so far absent.With the identification of the special markers of lymphatic vessel endothelial cell by immunohistochemical method in the recent years,the rapid progress in the research of lymphangiogenesis of the tumors had been made.This article briefly reviewed the progress of the special markers of lymphatic vessel endothelial cell and vascular endothelial growth factors in the differentiated neoplasm of the thyroid.