Objective:To study the effect ofRNA interferenc(RNA)ion FAK gene expression and the biological characteristics of human breast cancer MCF-7.Methods:The constructed recombinant plasmid FAK-siRNA was transfected into human breast cancer MCF-7 cell using LipofectamineTM 2000.The transfection efficiency was observed under fluorescence confocal microscope.Expression of FAK mRNA and protein were detected by reverse transcription polymerase chain reaction(RT-PCR)and Western blot.Distribution of cell cycle was assessed by flow cytometry.Cell penetrate matrigel capacity were determined by invitro experiment.Results:FAK-siRNA effectively inhibited FAK mRNA and protein expression(P

Developing along with the chemicals to tend gradually hard and adopting the natural medicine to rise,the international pharmacy field interesting in Chinese materia medica(CMM) increases gradually.The exterior competition of the CMM enterprises is becoming drastic and the secondary deve-(lopment) of CMM for the medicine enterprises in our country should be a better choose to face the international medicine market competition.The internal and external process about the secondary development of CMM should be taken into account and the strategy that should be adopted in the secondary development put forward.Such as: secendary development of new medicine of the CMM compound recipe;turning deeply to the CMM foundation research;the importance of developing the safe and valid CMM whose qua-(lity) is stabile and controlled;the importance of rising the processing level of the product,etc.It emphasized the importance in appling modern science and technology in the text.

Objective To study the curative effectiveness of external iliac-popliteal artery artificial graft cross bypass in treating extensive arterial occlusion in unilateral lilac-femoral arteriosclerosis. Methods From Sep. 1999 to Oct. 2007, 39 elder patients were diagnosed as having extensive arterial occlusion in unilateral lilac-femoral arteriosclerosis by color Doppler ultrasonography, CTA or DSA. 25 patients (61.5%) had resting pain, 14 had ulcers or gangrenes in the toes. The average ankle-brachial index was 0. 19. Contralateral external iliac-popliteal artery artificial graft cross bypass surgeries were performed on 22 patients from left to right, the other 17 were from right to left. Results There were no perioperative death or amputation. The ankle-brachial index increased from 0 ～ 0. 41 ( preoperative ) to 0. 85 ～ 1.02(postoperative) ; the claudication distance increased from 15 ～ 60 m (preoperative) to 350 ～ 500 m or more than 500 m [ postoperatively at the speed of (100 ～ 120) m/min] ; The postoperative color Doppler uhrasonography showed the average velocity of the popliteal artery was 45 cm/s. In the anterior tibial artery or the posterior tibial artery, little blood flow was showed in preoperative color Doppler ultrasonography examination, the postoperative average blood flow velocity was 41 cm/s. 35 patients (89. 7% ) were followed up for an average of 3.4 years, with an accumulative patency rate of 85.7%. Four patients had amputation, the limb salvage rate is 88.6%. Conclusion External iliac-popliteal artery artificial graft cross bypass is effective in treating unilateral lilac-femoral arterial occlusion, particularly for aged patients with vital organ incompetence.

Objective To investigate the correlation between the expression of p170、GST-π 、TOPO Ⅱ and the expression of Her-2 in human breast cancer. Methods The expression of Her-2 was detected by fluorescence in situ hybridization.The expression of p170,GST-π,TOPO Ⅱ were tested in 48 breast cancer by immunohistochemical SP method,and their correlations with clinicopathological features and the expression of Her-2 were analyzed.Results The positive rate of p170,GST-π,TOPO Ⅱ were 43.8 ％(21/48),39.6 ％(19/48),56.3 ％(27/48),respectively. The expression of p170, GST-π,TOPO Ⅱ positively correlated with the expression of Her-2(P＜ 0.05).The levels of p170,GST-π,TOPO Ⅱ expression were not associated with age,primary tumor and lymph node metastasis(P ＞ 0.05).Conclusion There is great possibility of chemotherapy drug resistance in breast cancer with expression of Her-2.Expressions of p170,GST-π,TOPO Ⅱ and Her-2 have an instructive significance for chemotherapy.

Papillary carcinoma is one of the commonest tumors in the thyroid.The pathologic diagnosis of papillary carcinoma as well as follicular carcinoma and follicular adenoma of the thyroid are based on the morphology observation hitherto.However,the diagnostic value of immunostaining for distinction among differentiated neoplasm of the thyroid was so far absent.With the identification of the special markers of lymphatic vessel endothelial cell by immunohistochemical method in the recent years,the rapid progress in the research of lymphangiogenesis of the tumors had been made.This article briefly reviewed the progress of the special markers of lymphatic vessel endothelial cell and vascular endothelial growth factors in the differentiated neoplasm of the thyroid.

Objective To study the effects of single low-dose intravenous injection of milrinone on plasma cardiac troponin I(cTnI) in rabbits with stunned myocardium.Methods Thirty rabbits with stunned myocardium were included in this study and they were randomly divided into three groups(10 rabbits a group).In sham-operated group(group A),the left anterior descending branch(LAD) of rabbits were not occluded.In ischemia and stunned myocardium group(group B),the LAD of rabbits were occluded for 15min and perfused for 60min.In milrinone group(group C),the LAD of rabbits were occluded for 15min,injected with low-dosage(5ug/kg) of milrinone,and perfused for 60min.Results The content of plasma cTnI in group C was decreased significantly than that in group B(P

BACKGROUND: The method and effect of construction of tissue-engineering heart valve scaffold with cell detergents are different, and the method of detergent combining with the trypsase and nucleese is more suitable than others. OBJECTIVE: To investigate the effect of different detergents (sodium deexycholate, sodium dodecylsulfate, and triton) combined with trypsase on decellularized porcine heart valve and the influence on the accellular scaffold. METHODS: Porcine aortic valve leaflets were sterilized by antibiotics for 12 hours, maintained in the solution of the trysin and the EDTA for 12 hours, and treated with sodium deexycholate, sodium dodecylsulfate, and triton. Finally, the sample was dip in nucleese solution for 12 hours to remove endothelial cells and interstitial cells. HE staining was used to detect whether the endothelial cells were removed completely, Masson staining was used to evaluate damage level of collagen fiber and elastic fiber, and electronic scanning was used to observe the microstructure. RESULTS AND CONCLUSION: All the three detergents completely removed the endothelial ceils; however, the effect of sodium decxycholate on collagen fiber and elastic fiber was light, and then sodium dodecylsulfate and triton. This suggested that the method of DOA combining with the enzyme digestion was a suitable technique to construct tissue-engineering heart valve scaffold.

As a new biotechnology, protein transduction connects the target fragment with biology active and protein transduction domain, and transducer it in to cells, exert its biological effects. Protein transduc-tion domain is a small molecule peptide, not only the protein transduction domain can penetrate through cell membrane, but could maintain the activity of its fusion protein. Protein transduction is an emerging biology technology, it has been applied into all aspects of cell gene regulation since its invention. In this article, we take Tat, Pdx-1 and Beta/NeuroD for instance and review the application of transduction protein in the dif-ferentiation of pancreatic stem cell.