Objective T o explore the num ber of necrophagous flies and seasonal distribution of com m on necrophagous flies at present in B eijing. Methods T he specim ens of necrophagous flies w ere collected by the m ethods of anim al carcass, trapping and feeding. A nd the specim ens w ere observed and counted after the classification and preservation. Results T he necrophagous flies in B eijing belonged to 4 fam i-lies, 9 subfam ilies, 21 genera and 46 species, and 12 species of them w ere the first records in B eijing. T he necrophagous flies had the characteristics of regional and seasonal distribution. Conclusion T he data of seasonal distribution of necrophagous flies and com m on necrophagous flies in B eijing can provide refer-ence for related research.

Objective To investigate the fly in relation to cadaver in the area of Guizhou. Method According to entomological investigation method, 12 areas were selected for investigation, where specimens were collected from 10th to 20th in February, May, August and November in a year. Results 37433 sarcosaphagous fly specimens were collected in 12 areas in a year, which were classified into 27 species of 17 genera in 5 families.Conclusion Data on the sarcosaphagous fly species, area- and season-related distribution were obtained, which is reported first time in China. and may form a basis for further study on the development of sarcosaphagous fly and provide reference to estimation of possible season and circumstance when death occurred.

The changes of calcium and magnesium content in brain tissue (BCa;BMg) and in the mitochondria (MCa,MMg) and calmodulin content in the cortex and hippocampus (CCaM,HCaM) were determined in the rats with 4-vessel-ligation cerebral ischemia.And then the therapeutic effects of nimodipine were observed during ischemic and.reperfusion stages of the brain.It was found that BMg and BMg were decreased and CCaM HCaM increased during both the ischemic and reperfusion stages and BCa and MCo were increased during the reperfusion stage.The fact that calcium content was only increased during the reperfusion stage indicates that the influx of calcium plays an important role to precipitate injury during the reperfusion stage.Adminstration of nimodipine could prevent the increase of calcium and calmodulin and the decrease of magnesium after cerebral ischemia.

Objective To explore the value of laparoscopic operations for early-stage cervix cancer. Methods Eighteen patients with Ⅰ b～Ⅱ a astage cervix cancer underwent extensive total uterectomy combined with pelvic lymphadenectomy under laparoscope (17Ⅰ b cases, 1Ⅱ a case). Concurrently, another 19 patients with the same diagnosis and approximately the same clinical features as the abovementioned 18 cases, as the control group, were treated by open extensive total uterectomy combined with pelvic lymphadenectomy. The operation time, intraoperative blood loss, postoperative recovery and complications of two groups were compared. Results No statistically significant differences were found on mean operation time between the laparoscopy group (272.8 min?80.3min) and open group (226.5 min?66.8min) (t=1.921,P=0.063). The number of removed lymph nodes was 15.6?5.1 in laparoscopy group and 16.8?5.7 in open group, without statistically significant differences (t=0.674,P=0.505). The recovery time of intestine functions in laparoscopy group (34.2 hours) was obviously shorter than that of open group (60.7 hours) (P

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Objective To evaluate the clinical efficacy of four different operative patterns of laparoscopic hysterectomy: laparoscopically assisted vaginal hysterectomy (LAVH), laparoscopically introfasial subtotal hysterectomy (LISH), laparoscopically subtotal hysterectomy (LSH) and laparoscopically total hysterectomy (LTH). Methods A retrospective analysis on 2272 cases of laparoscopic hysterectomy was carried out, including operating time, blood loss, complication and postoperative recovery. Results For the two groups which preserved cervix, LISH was performed in 1323 cases. The operating time was (91?21) min, blood loss (93?23) ml, complication rate 4.1%. LSH was conducted in 229 cases, with an operating time (70?18) min, blood loss (69?17) ml, complication rate 0. The difference between the two groups was significant (all P0.05). All patients recovered well postoperatively. Conclusions The four operative patterns are ideal for hysterectomy. Young patients should be operated with laparoscopic hysterectomy with preservation of cervix, old patients or patients with CIN should be operated with excision of cervix.

Objective To test the reliability and validity of CRIES-13(Chinese edition). The Children Revised Impact of Event Scale (CRIES-13) was recommened for diagnosing PTSD of children. Methods In the last third of the September 2008,according to the suffering condition,600 students were choosed who were fit for the research standard as subjects in two middle schools randomly. The viability of CRIES-13 was weighted by testretest reliability,Cronbach' s alpha,Split half reliability. The validity of CRIES-13 was analysed by content validity ,criterion validity,construction validity. Results In the test-retest reliability of CRIES-13, the Spearman correlation coefficient of total,intrusion factor,avoid factor,high warkening factor were 0.79, 0.75, 0.71, 0.75. Significant correlation were found among these scores. The Cronbach' s alpha of population was 0. 81. The Cronbach' s alpha of three factors was 0. 79 ( intrusion factor) , 0. 71 ( avoid factor), 0. 65 ( high awarkening factor). CRIES-13's split-half reliability was 0. 85. In the content validity test,the Spearman rank correlation coefficient between total score and each item was 0. 83 (intrusion factor), 0. 75 (avoid factor), 0. 85 (high awarkening factor). The correlation between intrusion factor and avoid factor was 0.63. The correlation between avoid factor and high awarkening factor was 0.41. The correlation between intrusion factor and high awarkening factor was 0.41. In struction validity, variance orthogonal rotation factor analysis was adopted and got three general factors. Their cumulative contribution to total variance was 55.52%. In the criterion validity test,significant correlation was found between intrusion factor and SDQ emotional factor and depression scale total score. Significant correlation was found between high awakening factor and SDQ emotional factor and depression scale total score. Conclusion The reliability and validity of CRIES-13 was good. It could be used extensively.

Objective The Children's Revised Impact of Event Scale (CRIES-13) is used for Screening PTSD of children. The reliability and validity of CRIES-13 is good. To research the demarcation points of CRIES-13 (Chinese version) based on the reliability and validity analysis,and to improve the useful value of the scale.Methods In late September 2008, according affected condition, students were choosed who were fit for the research standard as subjects in two middle schools. First,general questionnaire (self-writing) and CRIES-13 were applied to the subjects. Second, according to K-SADS-PL, physician carried out diagnosis meeting and evaluation to 310 students who were classified by stratified rand sampling. Critical point of CRIES-13 was divided by K-SADSPL. The assessment value of it were sensitivity, specificity, veracity, PPV, NPV. The right choice of division was measured by ROC curve. Results When the critical score was higher than 30, the score of Se ( 0. 833 ), Sp(0. 836) and NPV (0.97) was in the high level. Conclusion When the critical score is higher than 30, the scale have a good discrimination for PTSD, non-PTSD and it can be used extensively.

Objective To detect CD_(36) expressions in polycystic ovary (PCO), and to explore its correlation with local androgen and insulin at transcription level. Methods From August 2002 to February 2003, 12 patients with asymmetric PCO, 15 primary or secondary infertile patients without endocrine disorders and 8 polycystic ovary syndrome (PCOS) with bilateral PCO were recruited. Extraction of follicular fluid and detection of testosterone(T), dehydroepiandrosterone sulfate (DHEAS), insulin (INS) and androstenedione (A_2) were performed. Relative CD_(36) mRNA expression level of human ovarian inner thecal cells was analyzed by auto image analysis system (IAS) after RT-PCR. Results The level of CD_(36) mRNA expression in thecal cells was 0.24?0.07 in polycystic ovary of PCO group and 0.21?0.05 in bilateral ovaries of PCOS group, respectively, which were significantly lower than 0.83?0.13 in normal ovaries (P

Objective:To explore the characteristics of working memory ability of female methamphetamine dependent youths, and to analyze the effect of spatial and temporal training task (CODING) on working memory ability of female methamphetamine dependent youths.Methods:Sixty methamphetamine dependent youths from a women's drug rehabilitation center in Sichuan province were selected as the methamphetamine dependent group, while 60 female healthy volunteers from vocational high schools and universities participated as the healthy control group. Visual memory test (VISGED) was used to evaluat the working memory ability of the subjects, and SPSS 21.0 software was used for the nonparametric test of the memory difference between the two groups.The methamphetamine dependent group was divided into treatment group and waiting group based on the baseline matching principle of working memory. The treatment group was trained under the spatio-temporal CODING paradigm for working memory, and the training effect was assessed using the independent sample t-test. Results:(1)The individual parameters(0.06(-1.62, 1.67) vs 1.93 (-0.28, 2.71)) and working program(15.00(14.00 vs 16.00), 17.00(15.00, 20.00)) of working memory in methamphetamine dependent group and normal control group were statistically significant ( Z=-3.83, P<0.05, Z=-3.50; P<0.05). (2) Through memory training, the personal parameters before and after training in the treatment group ((0.08±1.79) vs 1.68 (0.68, 2.25)) had a statistically significant difference ( Z=-3.63, P<0.05). The personal parameters before and after the test in the waiting group ((-0.01±1.86) vs 0.72(-1.54, 1.88)) had no statistically significant difference ( Z=-1.35, P>0.05). Conclusion:Methamphetamine dependence impaire the working memory of female youths regardless of age or education level. Memory training can restore and improve memory ability to a certain extent, help to restore learning ability and living ability, and lay a foundation for a better return to society.