1.Inhibitive effects of genistein and quercetin on NIH3T3 fibroblast proliferation and collagen synthesis
Lihong QI ; Luping KANG ; Junping ZHANG ; Futang LIU ; Jianping ZHOU ; Bin ZHOU ;
Academic Journal of Second Military Medical University 1999;0(12):-
Objective:To study the effects of genistein (GE) and quercetin (QU) on proliferation and collagen synthesis of mouse fibroblast NIH3T3 cells.Methods:Cell proliferation was measured by crystal violet staining assay.Collagen synthesis was determined by 3H proline incorporation assay.Results:GE (25 70 ?mol?L -1 ) and QU (6.25 50 ?mol?L -1 ) concentration dependently reduced the serum driven increase of cell proliferation and collagen synthesis.GE and QU also attenuated platelet derived growth factor (PDGF) driven proliferative activity and transforming growth factor ?1 (TGF ?1) driven collagen synthesis.Conclusion:GE and QU can inhibit mouse fibroblast NIH3T3 cell proliferation and collagen synthesis in vitro .
2.Detailed resume of RNA m6A demethylases.
Dandan SHEN ; Bo WANG ; Ya GAO ; Lijuan ZHAO ; Yaping BI ; Jinge ZHANG ; Ning WANG ; Huiqin KANG ; Jingru PANG ; Ying LIU ; Luping PANG ; Zhe-Sheng CHEN ; Yi-Chao ZHENG ; Hong-Min LIU
Acta Pharmaceutica Sinica B 2022;12(5):2193-2205
N6-Methyladenosine (m6A) is the most abundant internal modification in eukaryotic mRNA, playing critical role in various bioprocesses. Like other epigenetic modifications, m6A modification can be catalyzed by the methyltransferase complex and erased dynamically to maintain cells homeostasis. Up to now, only two m6A demethylases have been reported, fat mass and obesity-associated protein (FTO) and alkylation protein AlkB homolog 5 (ALKBH5), involving in a wide range of mRNA biological progress, including mRNA shearing, export, metabolism and stability. Furthermore, they participate in many significantly biological signaling pathway, and contribute to the progress and development of cancer along with other diseases. In this review, we focus on the studies about structure, inhibitors development and biological function of FTO and ALKBH5.