Objective To investigate the myocardial injury in the early stage of acute dichlorvos poisoning in rats. Method A total of 24 Sprague Dawley rats were randomly (random number) divided into control group(n = 12) and poisoning group(n = 12). Hemodynamic variables were monitored by using an arterial cannula inserted into right arteria carotis communis. Serum levels of cardiac troponin T(CTnT) and brain natriuretic peptide (BNP) were measured. Myocardial tissue was observed with HE stain under microscope. Results The rats of poisoning group showed that the heart rate (HR) and maximum ascending rates of left ventricular pressure(+ dp/dtmax)were significant decreased in an hour after poisoning (P ＜0.01). The maximum descending rates of left ventricular pressure(-dp/dtmax)and left ventricular end diastolic pressure (LVEDP)were markedly increased (P＜0. 01) and reached peak in 7 minutes in the poisoning group. Compared with the control group, cardiac troponin T obviously changed in rats poisoned with dichlorvos in the first hour. BNP was not affected after poisoning(P ＞ 0. 05). Myocardial damage was found in the poisoning rats.Conclusions Myocardial injury and heart failure occurred in the early stage of acute organophosphorus pesticides poisoning(AOPP) in rats. CTnT could play a major role in AOPP while BNP might not be involved in.

Objective To observe the change of cerebral oxygen metablism and the level of blood lactate in early stage of sepsis in rats.Method Sixty-four SD rats were randomly(random number)divided into septic group and control group.The sepsis model of rat was made by lipopolysaccharide (LPS,10 mg/kg)injected intra-abdominally,and rats of control group were treated with the same amount of physiological saline instead.And each group was further divided into 4 sub-groups of4 h,6 h,12 h and 24 h after treatment.At each interval,blood samples were obtained via jugular vein for detecting blood oxygen saturation (Sjv02)and blood lactate(LA).The blood oxygen saturation(Sa02)of ventral aorta was also measured.Arteriovenous oxygen content difference (AVD02) and oxygen extraction fraction (OEF) were studied.These four variables were analyzed and compared between two groups.Results The AVD02 and OEF in sepsis group were higher than those in control groups of 3 h,6 h and 12 h (P<0.05) ,but lower than those in control group of24 hours (P<0.05).The Sjv02 in sepsis group was lower than that in control groups of 3 h,6 h and 12 h (P<0.05),but increased to that in control group of 24 h (P>0.05).LA in sepsis group was higher than that in control group in each interval (P<0.05).Conclusions In early stage of sepsis,cerebral perfusion,cerebral oxygen supply and cerebral oxygen extraction increase.The global oxygen metabolic variables(Sjv02,AVD02 and OEF)and the level of LA can be used to objectively and accurately evaluate the cerebral oxygen metabolic dysfunction in early stage of sepsis.

Objective:To investigate the protective effect of fasudil on the striated muscle in rats with acute organophosphorus pesticides poisoning(AOPP).Methods:The AOPP model was established by peritoneal injection of dichlorvos (DDVP).Rats were randomly divided into control group(n=8),poisoned group(n=8)and pretreated group(n=8).All rats in the 3 groups were supported with breathing machine connected with the tube from incision of trachea.The control group was peritoneally in-jected 0.9% sodium chloride solution (0.4 mL).The poisoned group and the pretreated group were peritoneally injected DDVP (30 mg/kg).The pretreated group was peritoneally injected fasudil(10 mg/kg)30 min before anesthesia.Serum level of cardiac troponin T(cTnT)was measured by using autonomic biochemical analyzer,and creatine kinase isoenzyme MM(CK-MM)and lactate dehydrogenase isoenzyme M(LDH-M)were measured by enzyme-linked immunosorbent assay(ELISA)2 h later.The expression levels of Rho-associated coiled-coil containing protein kinase I (ROCKI)mRNA of left ventricular myocardium and left gastrocnemius muscle were analyzed by reverse transcriptase polymerase chain reaction(RT-PCR).Results:Compared with the poisoning group,the striated muscle poisoning symptoms were less serious in the pretreated group.Compared with the con-trol group,the level of cTnT in the poisoning group and the pretreated group significantly increased(P <0.05).Compared with the poisoning group,the level of cTnT in the pretreated group significantly decreased(P <0.05).The levels of CK-MM and LDH-M were not obviously different among 3 groups.Compared with the control group,the expression level of ROCKI mRNA of left ventricular myocardium and left gastrocnemius muscle in the poisoning group significantly increased(P <0.05).Com-pared with the poisoning group,the expression level of ROCKI mRNA of left ventricular myocardium and left gastrocnemius muscle in the pretreated group was significantly inhibited(P <0.05).Conclusions:Fasudil can relieve symptoms of AOPP in rats.Fasudil has protective effect on the striated muscle in rats.