ObjectiveTo observe the effect of muscle stimulating instrument in enforcing muscle stone of children with cerebral palsy after selective posterior rhizotomy(SPR).Methods106 patients were divided into two groups,treatment group(n=51) and control group(n=55).Two groups received routine rehabilitation therapy,otherwise,treatmwnt group were treated with the muscle stimulating instrument after SPR.Pretreatment and 30d,60d,180d after treatment,muscle stone of two groups was measured and compared.ResultsImprovement of treatment group in muscle stone was better than that of control group(P<0.01).ConclusionMuscle stimulating instrument is effective to improve muscle tone of children with cerebral palsy after SPR.

ObjectiveTo observe the effect of selective posterior rhizotomy (SPR) on children with spastic cerebral palsy.Methods517 spastic cerebral palsy cases were operated on by SPR, and a following up was performed for 24 months. After operation, curative effect of SPR was examined and evaluated.Results298 cases had excellent effect (57.6%); 187 cases had good effect (36.2%).Conclusion SPR is very effective for children with spastic cerebral palsy.

Objective:To clone swine leukocyte antigen,class II,DO alpha (SLA-DOA) gene from Banna mini-pig inbred line (BMI) and detect its mRNA expression level in 19 important tissues.Methods:The complete eDNA sequence of SLA-DOA gene was cloned by RT-PCR method from BMI and the mRNA expression pattern in BMI important tissues was examined by semi-quantative RT-PCR method.Nucleotide and protein sequences of SIA-DOA were used to carry out bioinformatics analysis and construct the phylogenetic tree.Results:The eDNA length of BMI SLA-DOA was 1 079 bp,which encoded a protein of 250 amino acids with molecular weight (Mw) 27.81 kD,and isoelectric point (pI) 6.48.Genome structure analysis showed it localized to Sus scrofa chromosomes 7 and consisted of four exons and three introns.Semi-quantitative expression analysis showed that SLA-DOA gene expressed highly in the lymph nodes and stomach;weakly in the heart,skin and duodenum and none in other 14 tissues.Functional bioinformatics analysis indicated that SLA-DOA protein contained one signal poptide,one transmembrane region,three conserved domains,four O-GlcNAc glycosylation sites,18 potential phosphorylation sites and to be located in the cytoplasm with 94.1％ certainty.Phylogenetic analysis demonstrated that BMI (pig) had the closest relationship with cattle.Conclusion:This study have successfully cloned the SLA-DOA gene of Banna mini-pig inbred line,performed bioinformatics analysis and tissue expression profile analysis.It will provide a basis for the studies of BMI xenotransplantation.