Objective To evaluate the protective effect of genistein on psoralens plus ultraviolet A (PUVA)-induced photoaging in human dermal fibroblasts (HDFs) in vitro.Methods Dermal fibroblasts were isolated from the foreskin of a healthy 5-year-old boy,and subjected to primary culture.After 5-8 passages of subculture,the fibroblasts were collected and used in the following experiment.To determine the optimal concentration of genistein,methyl thiazolyl tetrazolium (MTT) assay was conducted to detect the proliferation of fibroblasts pretreated with 8-methoxypsoralen (8-MOP) and various concentrations (0-20 μg/ml) of genistein for 24 hours followed by UVA irradiation.Then,the fibroblasts were divided into 3 groups:normal control group receiving no treatment,photoaging group incubated with 8-MOP for 24 hours followed by UVA irradiation,and genistein group incubated with both 8-MOP and genistein at the optimal concentration for 24 hours followed by UVA irradiation.After additional culture,invert microscopy was carried out to observe the morphology of fibroblasts,enzyme histochemistry to assess senescent cells by using SA-β-Gal kit,flow cytometry to determine the level of reactive oxygen species (ROS),real-time fluorescence-based quantitative PCR to detect the matrix metalloproteinase-1 (MMP-1) expression.One-way analysis of variance was conducted to assess the differences in these parameters among these groups.Results At 24 hours after UVA irradiation,the percentage of fibroblasts positive for SA-β-galactosidase was (0.67 ± 0.58)％,(96.67 ± 1.53)％ and (51.67 ± 2.08)％ in the normal control group,photoaging group and genistein group respectively,with significant differences among these groups (P ＜ 0.01).The level of ROS in the photoaging group and genistein group was (0.88 ± 0.24) and (0.62 ± 0.02) fold higher than that in the control group(both P ＜ 0.01).Moreover,the MMP-1 expression level in the photoaging group and genistein group was 10 times and 6 times that in the control group,respectively,with significant differences among the 3 groups (P ＜ 0.05).Conclusion In vitro,genistein can protect against PUVA-induced photoaging in human dermal fibroblasts to some extent.

Objective To develop an HPLC analytic method for determination of cyromazine residue in water.Methods Chromatographic column of YMC C18(150 mm?4.6 mm,5 ?m)was used,mobile phase was methanol+0.02 mol/L NH4Ac=15+85(V/V),flowing rate was 1.0 ml/min,DAD detection wavelength was 210 nm.Results Regression equation was y=243.8x-4.2,r=0.999 96.The minimal detectable concentration was 0.010 mg/L;the rate of recovery was 94%-100% and the relative standard deviation was less than 3.0%.Conclusion The method is satisfactory in precision,accuracy and sensitivity.

Objective To study the contents of antibiotics and antiseptics in the cosmetics sold in markets. Methods The cosmetics sold in professional market and current market (in which cosmetics were retailed) were selected respectively in Guangzhou and the contents of four antibiotics (oxytetracycline, tetracycline, chlortetracycline and chloramphenicol) and five antiseptics (para-hydroxybenzcic acid, methyl para-hydroxybenzoate, ethyl para-hydroxybenzoate, propyl para-hydroxybenzoate and butyl para-hydroxybenzoate) were determined by high performance liquid chromatogram. Results In 111 cosmetics, 30 cosmetics were detected containing oxytetracycline, 62 cosmetics containing tetracycline, 7 cosmetics containing chlortetracycline and 1 cosmetic containing chloramphenicol. The detection rate was 78.4% and the detection rate of antibiotics among various kinds of cosmetics had no significant difference. The antiseptics used in cosmetics were mostly para-hydroxyl compound ester and there were four cosmetics detected containing antiseptics. Conclusion Abusing antibiotics in cosmetics market is very serious. The test standards and methods for antibiotics and antiseptics in the cosmetics should be developed as soon as possible. Cosmetic enterprises need to decrease microbe pollution in the process of production and prohibit adding antibiotics in cosmetics.

Objective An analytic method with rephase high pressure liquid chromatogrophy(RP-HPLC) was developed to determine the residue of deltamethrin in water.Methods Water sample was filtrated and determined by RP-HPLC.Chromatographic column was YMC C_(18)(250 mm?4.6 mm,5?m),mobile phase was methanol:water=90:10(V/V),the flowing rate was 0.8 ml/min, DAD detecting wavelength was 205 nm.Results The regression equation was y=655.6 x-1.8,r=0.999 99.The lowest detectable concentration was 2.9?g/L.The recovery rate was 98.0%-102.0%and the relative standard deviation was 0.29%-0.48%. Conclusion The method is proved to be satisfactory in precision,accuracy and sensitivity and can be used for the rapid determination of deltamethrin residue in water.

Objective To Study the method for determination of aluminium in water by spectrophotometry of Be ryllon Ⅲ. Methods Be ryllon Ⅲ reacts on aluminium in oppropriate temperature and acidity in water and produces a red complex compound?The red complex compound can be determined by spectrophotometry. Results The optimum reaction temperature was 85 ℃, the optimum reaction time was 5 min, pH of buffer was 6.1, Beer's law was obeyed in the concentration range of 0-0.02 mg/L for aluminium, rates of recovery were 80.0%-96.0% and RSD was 0.70%. Conclusion This method is accurate, simple, sensitive and repeatable.

The term of vascular cognitive impairment (VCI) has been constantly evolved and updated. The latest definition refers to the contribution of stroke and subclinical vascular brain injury that resulted from vascular pathology and its risk factors, to the cognitive impairment syndrome involving at least one domain, ranging from mild cognitive impairment to dementia, as well as mixed cognitive impairment, including Alzheimer′s disease. This article presents the epidemiology, etiology, pathogenesis, classification, clinical manifestations, auxiliary examination, diagnosis, prevention and treatment of VCI.

Neurofibromas are benign nerve sheath tumors that arise from the nonmyelinating Schwann cells. Generally, neurofibromas can be categorized into dermal and plexiform subtypes. The former subtype is usually associated with a lone peripheral nerve in the integumentary system, while plexiform tumors are associated with many nerve bundles and can originate internally. Rarely, the plexiform tumors can undergo malignant transformation. Neurofibromas are usually found in individuals with neurofibromatosis, which is an autosomal dominant disease. On occasion, an isolated neurofibroma can transpire without being associated with neurofibromatosis. Mostly, these solitary tumors tend to occur in the gastrointestinal system, and neurofibromas of the head and neck are not uncommon, but very rarely they have been reported to occur in the temporal fossa. In this report, we describe a case of a solitary neurofibroma arising from the temporal fossa.
Female ; Humans ; Middle Aged ; Neurofibroma ; Skull Base Neoplasms

Objective To study the risk factors for thyroid papillary carcinoma in patients aged over 65 years,and to investigate the etiology of thyroid papillary carcinoma.Methods A total of 784 patients with thyroid nodules screened by ultrasound were grouped according to different iodine intakes,gender,age,diet,smoking history and history of radiation therapy.The incidence of thyroid papillary carcinoma was compared among the different groups.Pathogenic factors for thyroid papillary carcinoma were analyzed.Results 55 patients (7.0％) were diagnosed as thyroid papillary carcinoma among 784 patients.Gender,age,iodine intake,smoking history and history of radiation therapy were the pathogenic factors for papillary thyroid carcinoma.Thyroid papillary carcinoma was common in older women,patients with high-iodine intake and patients with the history of radiation therapy.The incidence of thyroid papillary carcinoma was higher in patients with high iodine intake than with normal-iodine intake or low-iodine intake [22.8％ (31/136) vs.1.9％％ (10/517),10.7％ (14/131),x2 =75.07,P＜0.01].The incidence of thyroid papillary carcinoma was higher in female than in male elderly patients [8.0％ (49/612) vs.3.5％ (6/172),P＜0.05].The incidence of thyroid papillary carcinoma was higher in patients with the history of radiation therapy than without the history of radiation [11.6％ (14/121) vs.6.2％ (41/663),P＜0.05].Conclusions The main risk factors for thyroid papillary carcinoma are living environment,gender,age,diet,smoking history and history of radiation therapy,which are more influenced by external factors.

Objective To approach the clinical effect and safety of glutathione in patients with alcoholic liver disease(ALD).Method The patients were divided into two groups, including the control group and treatment group.Two groups are all treated with routine therapy such as Ganlixin,potassium magnesium aspartate,mulivitamins and so on,the treatment group add glutathione,to inspect clinical menifetation and liver function changes of two groups before and after treatments.Result Among the treatment group,43.6%showed notable effective result,51.3%effective,the total dffective rate is 94.9%. Among the control group, 19.4%showed notable effective result, 58.3%effective,the total dffective rate is 78.7%.There is a significant different between the two groups (P

ObjectiveTo investigate the expression and localization of midkine (MK) in human hepatocellular carcinoma. MethodsIn situ hybridization and immunohistochemical analysis for MK were performed on samples of both tumor tissues and paratumor tissues from HCC and benign liver tumors. ResultsThe distribution and localization of the MK transcripts′ signals determined by in situ hybridization were similar to those obtained by immunohistochemical analysis. Most HCC tissues showed enhanced positive reaction within cytoplasm to both MK probe and MK immunostaining. There was no significant difference in clinicopathological parameters between MK negative and positive cases of HCC. ConclusionsHuman hepatocellular carcinoma overexpresses MK at the mRNA and protein level.