A solid-phase radioimmunoassay (RIA) method was developed for detection of HBsAg. Its reprodu-cibility. sensitivity and specificity were evaluated, and these results were compared with the Abbott kit (AUSRIA).Horse anti-HBs was used both for coating solid phase and iodination. Normal horse serum was applied in the labelled antiserum to prevent the non-specific reaction between different species of animal sera.The cutoff was 2.21? negative control value. The factor 2.21 was derived from the mean S/N value and its 3 SD of 553 normal sera, while the factor of AUSRIA was 2.1. The lowest detectable amount of HBsAg by our RIA method was 4. 1ng/ml, while that by AUSRIA 2. 1ng/ml, which was about 2 times more sensitive than our system.The prevalence of HBsAg in normal population detected by this RIA ranged from 12.9 to 17.7%. that in the patients with acute viral hepatitis was 60.6%. and in chronic viral hepatitis patients 89.9%.As hepatitis B virus infection is quite common in our country, it is important and urgent to use RIA widely to detect HBsAg carrier in the clinical diagnosis, selection of donors and epidemiological investigation.

Objectives To evaluate the relationship between the expression of B7-1 in liver tissue and the effect of interferon-alpha(?-IFN) treatment in patients with chronic hepatitis B(CHB).Methods The expression of B7-1 in liver biopsy specimens from 68 CHB patients was studied with immunohistochemistry before ?-IFN treatment.Results B7-1 was expressed in 45(66 2%) liver tissues among 68 patients with CHB,but none in 5 normal controls.The total response ratio of ?-IFN in patients with B7-1 positive was 66 7%(30/45),which was significantly higher than 39 1%(9/23)in the patients with B7-1 negative (? 2=7 20,P

Objectives To evaluate the relationship between the expression of B7-1 in liver tissue and the effect of interferon- alpha(α- IFN) treatment in patients with chronic hepatitis B(CHB).Methods The expression of B7 -1in liver biopsy specimens from 68 CHB patients was studied with immunohistochemistry before α-IFN treatment.Results B7-1 was expressed in 45(66.2%) liver tissues among 68 patients with CHB,but none in 5 normal controls.The total response ratio of α- IFN in patients with B7-1 positive was 66.7%(30/45),which was significantly higherthan 39.1%(9/23)in the patients with B7-1 negative(x2 =7.20,P <0.01).B7-1 expression was closely corelat-ed with the histological activity grade(HAI) and serum alanine transaminase(ALT) level.Conclusions The level of B7-1 expression in liver tissue may be regarded as an effective parameter for predicting α-IFN response in patients with CHB.

Objective:To summarize the allergic mechanism caused by traditional Chinese medicine injection (TCMI) to provide some references for perfecting Good Laboratory Practice(GLP) of TCMI.Method:The related iteratures in data bases at home and abroad were reviewed,and the present experimental research methods about allergies were referred to to have a view of future studies.Result:The allergic mechanism of TCMI was mostly antigen-antibody reaction, part of which was anaphylactoid reaction.The method of the evaluation of allergy caused by TCMI only was animal experiments, but there were still some allergies caused by TCMI after the evaluation with this method.The present experimental research methods indicated that the detection of mediators of inflammation and FCM(Flow Cytometry) could be used to evaluate the allergies caused by TCMI.Conclusion:More attention should be paid to allergies caused by TCMI for its complicated mechanism and frequent occurrences in clinic.It may be an effective way to evaluate the allergies caused by TCMI with several methods including in vivo and in vitro.

Objective To observe the clinical effect of treatment of type 2 diabetes with Glargine and Novolin 30R.Methods68 patients from June 2015 to October 2016,were randomly divided into insulin glargine group and Novolin 30R each group 34 cases, According to the medical staff were given psychological counseling for patients to explain the importance of disease related knowledge and related treatment, timely eliminate/alleviate the negative emotion of patients with, to ensure that patients with a good attitude to face treatment;Follow-up and It occurred in January when the two groups were recorded hypoglycemia ratio, triglyceride (TG), total cholesterol (TC), 2h postprandial blood glucose (2hPG), glycated hemoglobin, the use of statistical methods for data analysis.ResultsThe proportion of patients with insulin glargine group hypoglycemia after treatment 2.94%, better than Novolin group 17.65%, and the difference was significant (P<0.05).②After treatment glargine group TG, TC index were (1.70±0.21) mmol/L, (5.24±0.16) mmol/L, were better than Novolin group (3.04±0.35) mmol/L,(5.58±0.22) mmol/L, and the differences were statistically significant (P<0.05).After treatment glargine group glycated hemoglobin was (7.02±0.18)%, lower than Novolin group (7.78±0.30)%, and the differences were statistically significant (P<0.05).ConclusionGlargine and Novolin 30R can effectively control blood sugar, low insulin glargine hypoglycemia event rate, the better the treatment of type 2 diabetes, is worthy of further research and application.

Objective To study the electrolyte status of 50 infants with severe pneumonia. Method By means of retrospective way to analyze the electrolyte level of blood sample from 50 young children. Results The sequences of electrolyte disturbances in the 50 young children with severe pneumonia were hyponatremia(34%), hypokalemia(30%),hypocalcemia(18%),hypochloremia(16%),hypernatremia(12%),and heperkalemia(10%). Conclusion Young children with severe pneumonia often have electrolyte disturbances of various kinds at the same time; electrolyte evaluations of blood samples from such young children should be done routinely, so that problems can be found early, and correcting measures be taken in time.

Objective To detect the virus in the feces and sera of patients in an outbreak of enterically transmitted non-A,non-E hepatitis,and this review covers the epidemiologic features and experimental infection of this novel virus.Data sources Data sources come from our own work on this subject,published and unpublished.Study selection Mainly our own work is included,and related literature is collected.Results In an outbreak of enterically transmitted non-A-E hepatitis among students,a total of 381 cases (60.7%)were documented.Viral fragments identical to transfusion transmitted virus(TTV)were detected in both serum and stool samples.Asymptomatic virus carriers among the staff had positive serum(32.1%)and feces(24.6%),clearly a potential source of infection.This viral infection prevalence in 2 remote villages in northern and southern China was 9.2% and 10.6%,respectively,suggesting that China is an endemic area.In this study,groups of 3 Rhesus monkeys were infected via oral or intravenous inoculation with patient feces.Two additional monkeys were infected by passage.The virus was detected in serum,peripheral blood mononuclear cell(PBMC),liver,spleen and small intestine,while the virus positive single strand,which might be a replicative intermediate,was only in liver,intestine and PBMC of all animals.Conclusions This nonenveloped DNA virus might be transmitted both by blood and enteric routes.Considering its wide distribution and high prevalence,we suppose that nonparenteral transmission is more important.

Objective To histomorphometricly assess changes occurred in the alveolar ridge following different methods of socket preservation and to compare them against natural healing without interventions. Methods The second、 third and fourth mandibular premolars were extracted from six beagles. Six extraction sites in each dog were randomly assigned to three treatments as follows:natural healing (T1), Bio-Oss Collagen (T2) and immediate implant with Bio-Oss (T3). Six month after surgery, the dogs were euthanized and tissue samples were sectioned, fixed and mounted, then were stained with toluidine blue. The histologic studies and morphological measurements were performed by using an optical microscope and a digital image software. Results Reabsorption in the buccal aspect of the alveolar crest of ridge was showed in all groups. With respect to the mean vertical bone loss of the buccal bone plate, T3 is lower than T1 and T2(P<0.001 ), while no significant differences were observed between T1 and T2. With regard to horizontal dimension of the alveolar process , a statistical significance could be found at 3mm and 4mm below the crest of ridge in group T1 and T3(P=0.017, P=0.042), while no statistical differences were found between other groups. Conclusions Both techniques of alveolar ridge preservation were not able to completely preserve the original bone volume after tooth extraction. Immediate implant placement in combination with Bio-Oss seems to have the potential to limit the reabsorption of the alveolar process efficiently , but the bone preserving effect of Bio-Oss Collagen is undesirable.

0.05 ),and the gastric evacuation rate in SDS group was lower than Group C(P

Objective To investigate the reproductive outcome after high intensity focused ultrasound (HIFU ) ablation for pa-tients with adenomyosis who have fertility requirements .Methods From October 2010 to June 2013 ,38 patients with adenomyosis who have fertility requirements treated by HIFU in our hospital were analyzed .Results In 38 cases ,the menorrhagia severity score ,the dysmenorrhea pain score and adenomyosis lesions after HIFU ablation were significantly different from the levels before HIFU treatment(P<0 .05) .Successful pregnancy occurred in 15 patients ,full-term birth was seen in 7 patients(three of Cesarean section ,four of vaginal delivery);the remaining patients accept routine prenatal examination .All pregnant patients showed no ab-normality .Conclusion Although large sample size study with long-term follow up is need ,based on our results ,HIFU ablation for patients with adenomyosis might improve the pregnancy rate safely .