Gene expressions of interleukin-6 receptor subunit gp130 and gp80 were investigated by competitive RT-PCR in the rat model of osteoporosis. A significant increase in expressions of gp130 and gp80 was found in bone marrow of osteoporotic rat, this change may be a possible mechanism in osteoporosis due to loss of estrogen.

Objective To upgrade ordinary gastrointestinal machine to a digital one which can be connected to PACS.Methods By adding extra camera lens,frame grabber and processing software,ordinary gastrointestinal machine was transformed.Results The updated machine could work in digital way with fully DICOM support.Conclusion By analyzing the X-ray imaging chain,a digitalization method of gastrointestinal machine is presented,which can server as a guideline to digital transformation of non-digital X-ray machines.

The systematic teaching does better to help students master and use what they have learned, and it will be good for avoidance of repeated contents and shortening teaching time. It can also help students find more interest in studying neurology.

Objective:To investigate the effect of catgut implantation at acupoint,Compound Erxian Decoction on levels of serum cytokine in ovariectomized rats.Methods:Sixty 3-month-old female Sprague-drawley rats,weighed 230?20g each,were divided into six groups,ten rats in each group.The first group were sham-operated(Sham-G),while the remaining groups,established by excising both sides ovary,were catgut-acupuncture group(CAG),Chinese herb group(CHG),catgut-acupuncture plus Chinese herb group(CCG),estrogen group(EG)and model group(MG).There wasn’t any therapy in sham-operated group and model group.After 12 weeks corresponding therapy,all groups were sacrificed to procure blood serum for testing the contents of E 2 ,GM-CSF,IGF-1,TNF-?by means of radioimmunoassay and both retral thighbone for testing bone mineral density(BMD) and bone mineral content(BMC)in each specimen.Results:The BMD and BMC of thighbone,E 2 and IGF-1 of serum and in CAG,CHG,CCG and EG was compared with Sham-G,there was no significant difference,but which was higher than MG(P

BACKGROUND:Cerebral ischemia tolerance can promote proliferation of autologous neural stem cells in the hippocampus of cerebral infarction rats, but panaxtrial saponins effects on the proliferation of autologous neural stem cells in the brain have not been reported. OBJECTIVE:To explore the relationship of panaxtrial saponins, ischemic preconditioning and proliferation of endogenous neural stem cells in the hippocampus of rats at 7 days after cerebral infarction, and to observe the effect on neurobehavioral scores of rats after cerebral infarction. METHODS:Fifty Sprague-Dawley rats were included and randomly divided into five groups:sham group, ischemia group, ischemic control group, ischemic preconditioning group, and panaxtrial saponins group. In the latter four groups, acute models of cerebral infarction were established using Zea-Longa method. In the sham group, only an incision was made on the neck. The focal-focal ischemic tolerance models were established with twice suture method in the ischemic preconditioning and panaxtrial saponins groups. Sham operation was instead of ischemic preconditioning in the ischemic control group. In the panaxtrial saponins group, rats were given intraperitoneal injection of 100 mg/kg panaxtrial saponins at 7 days before modeling. RESULTS AND CONCLUSION:After 7 days of cerebral infarction, the neurobehavioral score and the number of neural stem cells in the hippocampus were significantly increased in the ischemia group (P<0.01);compared with the ischemia group, the neurobehavioral scores were lowered in the ischemic preconditioning and panaxtrial saponins groups (P<0.01), while the number of neural stem cells in the hippocampus was increased (P<0.01). However, there was no difference between the ischemic preconditioning and panaxtrial saponins groups (P>0.05). In addition, differences in the neurobehavioral scores and the number of neural stem cells in the hippocampus were insignificant between the ischemic control group and ischemia group (P>0.05). These findings indicate that panaxtrial saponins can play a role similar to ischemic tolerance, and thus improve neurologic impairment in rats with cerebral infarction.

Diabetes and lung cancer are common diseases worldwide , which have a serious impact on human health .Recently epidemiologic research has found that diabetes , which may enhance the incidence of lung cancer , is one of the risk factors of lung canc-er.The metformin, a first-line drug for treatment of type 2 diabetes, can decrease the incidence of lung cancer and may have an anti-lung-cancer bioactivity ,but its mechanisms are unclear .To make a further study of the mechanisms is expected to reduce the morbidity and is possible to help us find a new therapeutic target .The aim of this article is to review the potential mechanism of the metformin′s anti-lung-cancer activity .

Objective To investigate the effect of brain ischemic preconditioning (IP) combined with traditional Chinese medicine three seven three alcohol saponin (PTS) on proliferation of endogenous neural stem cells and the mRNA expressions of delta opioid receptor (DOR),Bax,Bcl-2 in hippocampus at 7d post middle cerebral artery occlusion (MCAO).Methods The focal-focal ischemic tolerance models were established with twice suture method.80 SD rats were included and randomly divided into 5 groups:sham group,MCAO group,sham+ MCAO group,IP+ MCAO group,PTS+MCAO group (n=16 each).We chose 10 SD rats from each group to evaluate their neurological status,and made BrdU fluorescent immunolabeling.In addition,we chose the other 6 SD rats to detect the expression levels of DOR,Bax and Bcl-2 mRNA in ischemic region in hippocampusby using RT-PCR.Animals were given one set of BrdU injections (on day 6,three times,4h apart,50mg/kg) to label the proliferating cells.The neurological status was assessed by using Zea Longa neurological deficit scores at 7 days following cerebral infarction.Results Zea longa neurologic deficit scores in MCAO group and sham+ MCAO) group had significantly differences with IP+ MCAO group and PTS+ MCAO group respectively at 7d post MCAO(P＜0.01).There was no significant differeuce in Zea-longa neurologic deficit scores between MCAO group versus sham+ MCAO group,and IP+ MCAO group versus PTS+ MCAO group(P＞0.05).The number of BrdU+ ceils in hippocampus had significant differences between IP+ MCAO and PTS+ MCAO groups at 7d post MCAOand three groups of sham,MCAO and sham+ MCAO respectively (P＜0.01).There was no difference in the number of BrdU+ cells between MCAO versus Sham + MCAO groups and IP + MCAO versus PTS+MCAO groups(P＞0.05).DOR and Bcl-2 mRNA expression levels were higher and Bax mRNA expression level was lower in IP+ MCAO group than in MCAO,Sham+ MCAO and PTS+MCAO groups (P＜0.01).There were no significant differences in DOR,Bcl-2 and Bax mRNA expressions among MCAO,Sham + MCAO and PTS + MCAO groups (P＞ 0.05).Conclusions Acute cerebral infarction can induce the proliferation of endogenous neural stem cells in hippocampus in SD rats.IPC can facilitate the proliferation of endogenous neural stem cells in hippocampus afteracute cerebral infarction,improve the symptoms of neurologic dysfunction,increase DOR and Bcl 2 mRNA expressions,and reduce Bax mRNA expression in SD rats.PTS can facilitate the proliferation of endogenous neural stem cells in hippocampus after acute cerebral infarction in SD rats,and improve the symptoms of neurologic dysfunction,but it has no influence on the expressions of DOR,Bcl-2 and Bax mRNA.

Objective To monitor lung growth rule of normal middle-late pregnancy fetal. Methods 599 cases of 18 and 40 weeks normal singletons were grouped by every two weeks. The length of right lung in the sagittal plane and the cross-sectional plane were measured, and the growth curve was described. The ratio of right lung area (RLa) and head circumference (Hc),chest circumference (Tc) and of the chest area (Ta) in different gestational ages was calcalated. The correlation coefficient was obtained by applying statistical analysis. Results There was a linear relationship between RLa/Hc and RLa/Tc and growth of gestational age;the regression equation was:(RLa/Hc: ?Y= 0.948X-1.221, r2 =0.898, the RLa/Tc: ?Y=0.944X-1.800, r2 =0.894). RLa/Ta in different ges-tational age was in constant range:0.42~0.55. The length of right lung in the sagittal plane and the cross-sectional plane increased along with the gestational age, and the growth speed was:before and after diameter(the cross-sec-tional plane) > upper and lower diameter (sagittal plane) > about size (the cross-sectional plane). Conclusion Prenatal ultrasound can evaluate the growth rule of normal fetal lung and provide the basis for assessment of fetal lung maturity.

The study is to describe the main activities of NGOs involved in programs of China AIDS Global Fund Round 5 and analyze the advantage and necessity of their involvement in HIV/AIDS control. The experience and problems of NGOs ' involvement in AIDS Global Fund Programs,as well as appropriate approaches to address them are discussed.