1.Seroprevalence of bluetongue virus infection in goats in the central China
Tropical Biomedicine 2017;34(1):80-83
Seroprevalence of Bluetongue virus (BTV) in goats from Hubei was investigated
by a commercial competitive enzyme-linked immunosorbent assay kits. Blood samples (n=
1157) were collected during the year 2014 and 2015. The results showed that 13.31% (CI 95%
11.4%–15.4%) serum samples were positive for BTV antibodies in goats in Hubei. The prevalence
of BTV antibodies in each region ranged from 1.32% to 27.70%, and differences among the
regions were statistically significant (p < 0.01). The prevalence of BTV in male and female
goats was 14.23% (95% CI: 11.3, 17.6) and 12.58% (95% CI: 10.1, 15.4), respectively, no
significant difference in genders (p > 0.05). In different seasons, the seroprevalence were
8.94% (95% CI: 5.6, 13.3) in spring; 18.31% (95% CI: 14.5, 22.7) in summer; 23.08% (95% CI: 17.0,
30.2) in autumn and 6.98% (95% CI: 4.6, 10.0) in winter, respectively with a significant
difference of the prevalence in the different seasons (p < 0.01).
2.Seroepidemiology of Toxoplasma gondii and Neospora caninum infections in goats in Hubei province, China
Luo, H.Q., Tu, Y.Q., Song, X.Z.and Sun, S.W.
Tropical Biomedicine 2016;33(2):285-289
Scarce information is available about the seroprevalence of Toxoplasma gondii
(T. gondii) and Neospora caninum (N. caninum) infections in goats in Hubei province,
China. In the present study, the prevalence of T. gondii and N. caninum infections in goats
were investigated in Hubei province, China between 2014 and 2015. A total 2007 serum
samples were collected from 6 counties of Hubei province, China and were tested for antibodies
to N. caninum and T. gondii by an enzyme-linked immunosorbent assay (ELISA) and an
indirect agglutination test (IAT), respectively. Antibodies against T. gondii and N. caninum
were detected in 13.4% and 3.9%, respectively in goats. 2% (41) serum samples were positive
to both parasites. There was no apparent association of T. gondii and N. caninum infection
with gender of the animals. There were significant differences of T. gondii (p < 0.01), N.
caninum (p < 0.05) and both parasites (p < 0.01) infection with season. This is the first time
that antibodies to T. gondii and N. caninum have been detected in goats in Hubei province,
China.
3.Molecular characterization of ascaris from Tibetan pigs by three mitochondrial markers of nad1, cox1 and cox2
Luo, H.Q. ; Zhang, H. ; Li, K. ; Lan, Y.F. ; Wang, X.Q. ; Khalid, M. ; Mujeeb, R. ; Huang, S.C. ; Li, J.K.
Tropical Biomedicine 2017;34(3):576-582
Ascaris is a helminthic parasite, which infects a wide range of host species
causing ascariasis, a predominant disease worldwide. This parasite causes significant economic
losses to the pig industry. The current study was designed to determine the Ascaris nematode
by the genetic characterization of three mitochondrial (mt) genes namely NADH dehydrogenase
subunit 1 (nad1), cytochrome oxidase subunit 1 (cox1) and cytochrome oxidase subunit 2
(cox2). A high infection rate of Ascaris nematode has been found in Tibetan pigs at the
slaughter houses in Tibet Autonomous Region of China. The nad1, cox1 and cox2 genes
sequences collected from adult Ascaris individuals were amplified by polymerase chain
reaction. The cloned-amplicons and the positive products were sequenced and phylogenetic
analysis was performed. The results indicated that the Ascaris infecting the Tibetan pigs
were Ascaris suum (A. suum). This is the first report on the isolation, identification and
genetic characterization of three mitochondrial genomes (nad1, cox1, and cox2) of A. suum
originated from Tibetan pigs at high altitudes in Tibet.
4.Epidemiological investigation and risk factors of Peste des petitis ruminants (PPR) in yaks (Bos grunniens) and cattle in five regions of China
Li, X.H. ; Li, K. ; Zhang, H. ; Gan, P. ; Luo, H.Q ; Han, Z.Q ; Mehmood, K. ; Shahzad, M.
Tropical Biomedicine 2018;35(3):736-743
To investigate the prevalence of Peste des petitis ruminants in yaks and cattle in
poorly studied areas of China. A total of 1202 and 560 blood samples were collected from
yaks and cattle, respectively and processed using a commercial ELISA kit. Multivariable
logistic regression model was piloted to find the variables, potentially associated with exposure
of PPR infection in yaks and cattle. Results indicated that the overall prevalence of PPR in
yaks was 11.2%. A total 66 (11.8%) out of 560 were examined out to be positive to PPR in
cattle in Jiangxi province. According to conditional stepwise logistic regression, region, age
and gender were found to be the more influencing risk factors in yaks, while region and age
were found to be the potential risk factors in cattle. The current results reported the prevalence
and associated risk factors of PPR in bovine for the first time in China.
5.Seroprevalence of Toxoplasma gondii infection in people in southeast Hubei province, China
Shen, Zh. Zh. ; Li, K. ; Li, Z.J. ; Shang, X.L. ; Hu, F. ; Zhou, W.J. ; Wang, H.L. ; Luo, H.Q.
Tropical Biomedicine 2020;37(No.2):452-457
Toxoplasma gondii is a world-widely spread zoonotic parasite. However, scarce knowledge is known about the prevalence of T. gondii infection in people in Hubei province, China. This study herein was to perform epidemiological investigation of T. gondii infection in people in this region. A total 12527 blood samples were obtained during 2015-2018, and were assayed for T. gondii antibodies of IgG and IgM, respectively by employing an indirect hemagglutination test (IHA). The results discovered that the prevalence of T. gondii in people was 2.44% and 6.1%, respectively based on antibodies of IgG and IgM, respectively. The prevalence was ranged from 0.3% to 5.4% during 2015-2018 based on IgM antibodies. For genders, the prevalence was 0.7% and 2.6% in males and females, respectively based on IgM antibodies. In different years, the prevalence was ranged from 4.9% to 14.0% based on IgG antibodies. The prevalence of T. gondii was 4.9% and 6.6% in males and femalesy based on IgG antibodies. The current results may be helpful for the implementation of preventive measures against Toxoplasma infection among people living in this region.