Thirty cases of pathological changes in placenta as a result of inducing labour with rivanol at the middle period of pregnancy. The results of the study indicated that the pharmacological action of rivanol is correlated with its chemical structure,which caused a reduction in the concentration of placenta hormone ( including HCG, pregesterone & estradiol )

Objective To establish a method for the separation and purification of total saponins from Radix Clematidis by column chromatography of macroporous absorptive resin. Methods Radix clematidis was extracted with 70 % ethanol and then the extraction was purified with D101 macroporous absorptive resin. After eluting with distilled water and 30 % , 50 % and 70 % ethanol, absorption capability and elution parameters of macroporous absorptive resin were measured by the indexes of elution rate and refinement rate of total saponins. Results Total saponins of Radix Clematidis were enriched in the 50 % ethanol eluted solution fraction. The elution rate of macroporous absorptive resin was 71.26 % and absorption capability was 53.36 mg/g . Conclusion It is feasible to use macroporous absorptive resin for the isolation and purification of total saponins from Radix Clematidis. The optimum elution condition is: removing water- soluble impurity by water, eluting total saponins with 50 % ethanol.

Objective To study the anti-inflammatory and immunity effects of Huatan Jiangqi capsule. Method The anti-inflammatory and immunity effects were investigated by ear swell model of mice induced by dimethylbenzene,granulation tissue hyperplasia model of rats,viscera coefficient and swimming time of mice. Results Huatan Jiangqi capsule could reduce ear swell degree of mice and granulation tissue hyperplasia of rats. It could also increase viscera coefficient and prolong swimming time of mice. Conclusion Huatan Jiangqi capsule had significant anti-inflammatory and immunity effects.

Objective To investigate potential mechanism of decreasiy leptin level of Pseudostellaria Polysaccharides on experimental diabetic rats. MethodsThe diabetic rats were induced by the abdominal cavity injection of alloxen(ALX) 200 mg· kg-1. The levels of leption and TNF-α were determined. Results Pseudostellaria Polysaccharides (0.6 g·kg-1 , 1.2 g·kg-1 ) could increase Leptin level and could decrease TNF-α level of DM rats(P＜0.05 or P＜0.01). Conclusion Pseudostellaria Polysaccharides can play an threpedtic rale in the experiment aldialetic rats through multi-pathway the experimental diabetic rats.

OBJECTIVE: To establish the quality standard of Compound buwu syrup. METHODS: TLC was employed to identify Psoralea corylifolia,Angelica sinensis,Ligusticum chuanxiong and Salvia miltiorrhiza.HPLC was applied for the determination of the contents of psoralen and isopsoralen.RUSULTS:Psoralea corylifolia,Angelica sinensis,Ligusticum chuanxiong and Salvia miltiorrhiza were all well-separated with clear TLC spots.The linear ranges of psoralen and isopsoralen were 0.030 4～0.304 ?g(r=0.999 1) and 0.036 2～0.362 ?g(r=0.999 5),respectively and the average recoveries were 100.1%(RSD=2.9%) and 102.4%(RSD=2.7%),respectively.CONCLUSION:The established standard is applicable for the quality control of Compound buwu syrup.

Objective To establish a method for determination of chrysophanol,emodin and rhein in Huangzhi capsules.Method Chrysophanol,emodin and rhein in Huangzhi capsules was determined by TLC-scanning with a mixture of n-hexane-ethylacetate-formic acid(30∶10∶0.5) as the developing system.The detection wavelength was 435 nm and 610 nm.Results The calibraction curve of chrysophanol,emodin and rhein was linear in the range of 0.014 65～0.073 25,0.013 35～0.066 75,0.012～0.072 ?g respectively.Conclusion The method was simple,accurate with good repeatability,and suitable for the content determination of chrysophanol,emodin and rhein in Huangzhi capsules.

Objective To establish an HPLC method for the determination of Aloeemodin,Rhein,Chrysophanol,Emodin and Physcione in Huangxiong Kangshuan Capsule.Methods The determination was performed on Kromasil-C18 column(250 mm?4.6 mm,5 ?m) with mobile phase consisted of methanol-0.1% phosphoric acid(85:15) at a flow rate of 1.0 mL/min.Column temperature was 25 ℃.The UV detection wavelength was set at 254 nm.Results The calibration curves were linear in the range of 0.230～3.68 ?g(r=0.999 8) for Aloeemodin,0.224～3.584 ?g(r=0.999 9) for Rhein,0.223～3.568 ?g(r= 0.999 8) for Emodin,0.257～4.112 ?g(r=0.999 9) for Chrysophanol and 0.287～4.592 ?g(r=0.999 8) for Physcion.The average recovery was 98.48%,98.10%,99.08%,100.34%,97.52% and RSD was 1.39%,1.54%,1.44%,2.40%,2.03% respectively.Conclusion The determination method is simple,reliable,accurate,and it can be used for quality control of Huangxiong Kangshuan Capsule.

Objective To establish the quality standard of Yuling Tea for its quality control. Methods Astragalus, Fructus cnidii L, Schisandra chinensis and Lycii Fructus in Yuling Tea were identified by TLC. The content of Schisandrin was determined by HPLC. Welch Materials C18 column (250 mm×4.6 mm, 5 μm) with mobile phase of methanol-water (70∶30) was used. The detective wavelength was 250 nm. Results The TLC for identification was simple and special. Schisandrin showed a good linear relationship in 0.222-0.222 0 μg, r=0.999 9. The average recovery was 98.96%, and RSD was 0.74%. Conclusion The method can accurately determine the content of Schisandrin in Yuling Tea. It can be used for quality control of the preparation.

ObjectiveTo optimize Chonglian oral solution extracting craft. MethodsWith the obtaining rate of extract and the total content of the Pariphyllin Ⅰ and Phillyrin presented in the extract as the indexes for the water extraction process,and with the total content of the Pariphyllin Ⅰ and Phillyrin presented in the extract as the indexes for the alcohol deposition process,orthogonal design was used to optimize the conditions for the extraction process respectively. ResultsThe optimal conditions for the water extraction of Chonglian oral solution was as following:to add water 10 times,decocting 3 times,1 hour each time.The optimal conditions for the alcohol deposition of Chonglian oral solution was as following:concentrated for the relative density to 1.10(80C hot test),cold,add ethanol to the solution for the ethanol content of the solution reached 80%,static settlement for 24 hours. ConclusionThe extracting method is reasonable,stable,and suitable to industrialized producting.