Objective To observe the expression of brain derived neurotrophic factor(BDNF) mRNA after nerve trunk stimulation by electroacupuncture in rats' cortex with cerebral ischemia/reperfusion. Methods The MCAO model was established by ligation of the artery for 1 hour, and then reperfusion was conducted for 2h, 6h, 12h, 24h, 2d, 3d, 7d and 14d.The expression of BDNF mRNA after the cerebral ischemia/reperfusion injury (MCAO group) was observed and comparison was made between the electroacupuncture group and the control group. Results The number of cells with positive expression of BDNF mRNA in the cortex after 2h, 6h, 12h, 24h, 2d and 3d of reperfusion was more than that in the control group (all P0.05). At 2h of reperfusion, the amount of the positive cells peaked and was much more than that at the 6h and 12h time points (P0.05),but after 6h reperfusion, a significant increase of expression of BDNF mRNA was observed against the MCAO group and control group, and the increase lasted to 14d after reperfusion. Conclusion Nerve trunk stimulation with electroacupuncture could improve and maintain the expression of BDNF mRNA for a long time, and this might contribute to the protection effect of electroacupuncture against the brain ischemia/reperfusion injury.

Objective To study the differences of clinical characteristics in the children patients with pneumonia under differ‐ent body mass indexes(BMI) to provide the basis for its clinical research .Methods 160 children patients with pneumonia in the Shandong Armed Police Corps Hospital were selected and divided into the normal BMI group (15 .00

The law named The Safety Management Standardization of Medical Apparatus and Instruments in Clinic issued by the government recently, has classified the technical evaluation as one of the aspects of controlling medical risks in demonstration of medical equipments purchasing. This article has researched the organization behavior model, put forward the inevitability of the role in clinical engineering subjection, provided the idea and method of medical equipment purchasing plan, corresponding with the new standardization in now period.
Purchasing, Hospital ; methods ; organization & administration