Objective To investigate the clinical effect of psychological intervention combined with insulin, glimepiride in the treatment of patients with diabetes mellitus. Methods 60 cases of patients with diabetes treated in our hospital from January 2015 to January 2017 were divided into two groups.The control group was treated with routine nursing intervention and insulin treatment, the observation group was treated with psychological intervention+insulin+glimepiride therapy; The clinical efficacy and clinical symptoms in the two groups were compared. Results The clinical effect of the observation group was better than that of the conventional control group,the clinical symptoms improved was better than the control group, the difference was statistically significant (P<0.05). Conclusion The clinical effect of psychological intervention+insulin+glimepiride in the treatment of diabetes is significantly, can effectively improve the clinical symptoms of patients, is widely used in clinical treatment of patients with diabetes.