1.Effect evaluation of nursing intervention to reduce the incidence of adverse events of iodine contrast agent extravasation
Lufang LI ; Yanxin WANG ; Hui WANG
Chinese Journal of Practical Nursing 2011;27(30):42-43
Objective Through analyzing the causes of iodine contrast agent extravasation,nursing managers develop effective solutions timely and take appropriate precautionary measures to ensure the safety of patients effectively.Methods 600 patients undergoing CT contrast enhanced scan were selected,300 patients from May 2009 to April 2010 were set as the control group and were given routine nursing care,300 patients from May 2010 to April 2011 were set as the study group and they adopted effective preventive measures.The incidence of adverse events after injection of iodine contrast agent between the two groups was compared.Results The incidence of iodine contrast medium extravasation in the study group was significantly reduced to 2% after taking a series of preventive measures,which was lower than 10% of the control group.Conclusions Taking a series of preventive measures can reduce the incidence of adverse events of iodine contrast agent extravasation,ensure the safety of patients,decrease the frequency of nursing complaints and nursing deficiencies,as well as improve the quality of nursing service.
2.Nursing cooperation in gastric coronary vein embolization utilizing NBCA glue
Hui WANG ; Fang LI ; Lufang LI
Chinese Journal of Practical Nursing 2012;28(23):8-9
Objective To investigate nursing cooperation during gastric coronary vein embolization procedure with NBCA glue. Methods Perioperative nuusing experience in 36 hepatic cirrhosis patients complicated with esophagus-gastric fundus vein varicose rupture and hemorrhea who had undergone gastric coronary vein embolization procedure was retrospectively summarized. Results Operations in all 36 cases had been successful,with no severe complication due to inadequate nursing cooperation. Conclusions Gastric coronary vein embolization operation utilizing NBCA glue is a safe and effective method of hemostasis,skilled and elaborative nursing cooperation is an important component ensuring a smooth and effective operation.
3.Mixed culture of Madin-Darby canine kidney cells, human epidermoid cancer cells and African green monkey kidney cells for detection of common respiratory viruses and enteroviruses
Yingyang GAO ; Chenyan JIANG ; Lufang JIANG ; Qianli WANG ; Liwen JU
Chinese Journal of Infectious Diseases 2011;29(6):321-324
Objective To establish a clinical test assay for detecting common respiratory viruses and enteroviruses (EV) by using mixed cultured Madin-Darby canine kidney cells (MDCK), human epidermoid cancer cells (Hep-2) and African green monkey kidney cells (Vero) to isolate common respiratory viruses and enteroviruses. Methods Throat swabs with influenza A and B viruses,adenovirus and EV71 were incubated with mixed cultured MDCK, Hep-2 and Vero in a single vial to observe the presence of cytopathic effects. Polymerase chain reaction (PCR), reverse transcription (RT)-PCR and monoclonal antibody-based immunofluorescene assay were also used for confirmatory test. Results The sensitive cell lines developed obvious cytopathic effects to the corresponding viruses, which were confirmed by the specific green particles observed by immunofluorescence assay and specific target PCR segments. Conclusions The shell-vial of mixed cells can simultaneously isolate different common respiratory viruses and EV. The isolated pathogens can be further confirmed by antigen test and PCR. This assay may improve the diagnosis of clinical viral diseases.
4.Clinical analysis of two different chemotherapy regimens for concurrent chemoradiotherapy in advanced cervical cancer for Uyghur women
Lufang WANG ; Nana HAN ; Mingfang RU ; Kaijiang LIU
The Journal of Practical Medicine 2014;(18):2928-2930
Objective To compare theshort-term efficacy and safety between TP and PVB scheme chemoradiation for advanced cervical cancer. Methods Between January 2012 and January 2014, 187 Xinjiang uygur patients with advanced cervical cancer (stageⅡb to Ⅳa) who received concurrent chemoradiotherapy in Xinjiang medical university affiliated tumor hospital were analyzed. A1l cases were divided into two groups receiving radiotherapy concomitant (n = 104), PVB group (n = 83). Theshort-term efficacy and toxicity was evaluated four weeks aftertreatment. Results For squamous cell carcinomas, the response rates were 85.9% and 73.5% in TP and PVB group respectively (P > 0.05). For Non-squamous cell carcinomas (adenocarcinoma and adenosquamous), the response rates were 75.7% and 40% in TP and PVB group respectively (P < 0.05). B one marrow suppression, neurotoxicity, gastrointestinal reactionwere significant different between two groups (P <0.05). Liver and kidney functiondisorder, blood cellsreduction, and radiation damagewere not significantly different (P > 0.05). Conclusions For cervical squamous cell carcinomas, theshort-term efficacy of two chemotherapy regimens is similar while forNon-squamous cell carcinomas, TP regimengroup is superior toBone marrow suppression and neurotoxicity in TP regimengroup is significantly higher than PVB group (P < 0.05), while gastrointestinal adverse reaction lower than PVBgroup.
5.Effect of puncture-related pain on the quality of life in patients undergoing maintenance hemodialysis through internal arteriovenous fistula
Lina GONG ; Jia LIU ; Jin YAN ; Lufang WANG
Journal of Central South University(Medical Sciences) 2014;(12):1292-1298
Objective: To investigate the effect of puncture-related pain on the quality of life in patients undergoing maintenance hemodialysis through internal arteriovenous if stula. Methods: A total of 180 hemodialysis patients with the arteriovenous ifstula were surveyed by the kidney disease quality of life short form(KDQOL-SF1.3), demographic data questionnaire, visual analogue scale and pain self-effcacy questionnaire. Results: The median score of puncture-related pain was 5 and the score of pain self-efficacy was (31.42±14.59). The quality of life in the patients undergoing maintenance hemodialysis is poor. KDQOL-SF1.3 was (69.45±24.19), SF-36 was (49.82±19.17) and ESRD-targeted was (55.46±18.37). Multivariate analysis demonstrated that the quality of life was positively correlated with the patient gender (β=0.152,P<0.05, OR=1.638, 95% CI 1.241–1.954), working position (β=0.307,P<0.05, OR=2.069, 95% CI 1.206–-3.148), using time of arteriovenous ifstula (β=?0.815, P<0.05, OR=0.223, 95% CI 0.095–0.741), the score of pain (β=-0.017,P<0.05, OR=1.004, 95% CI 0.886–1.431) and pain self-effcacy (β=-0.409,P<0.05, OR=0.803, 95% CI 0.710–0.984). hTere existed negative correlation between the quality of life score and the puncture-related pain score in these patients (r=-0.472,-0.465,-0.381,P<0.01), positive correlation between the quality of life score and the score of pain self-efficacy (r=0.647, 0.203, 0.518,P<0.05), and negative correlation between the puncture-related pain score and the score of pain self-effcacy(r=-0.745,P<0.01). Conclusion: Puncture-related pain is a crucial inlfuential factor on the quality of life in the patients undergoing maintenance hemodialysis through internal arteriovenous ifstula.
6.Analysis of the correlated factors of puncture-related pain in patients with the arteriovenous fistula undergoing maintenance hemodialysis
Jia LIU ; Liu SHAN ; Jin YAN ; Lufang WANG
Chinese Journal of Practical Nursing 2015;(25):1885-1889
Objective To investigate the current situation of puncture-related pain in patients with the arteriovenous fistula undergoing maintenance hemodialysis and its correlated factors. Methods A total of 180 hemodialysis patients with the arteriovenous fistula undergoing maintenance hemodialysis were investigated with demographic data questionnaire, visual analogue scale and pain self-efficacy questionnaire. Results The effective questionnaires rate was 93.89%(169/180). The median score of puncture-related pain was 5 scores, the score of pain self-efficacy was (31.42±14.59) scores;multivariate analysis demonstrated that the puncture-related pain was positively correlated with the patient gender, using time of arteriovenous fistula and pain self-efficacy ( β=0.152,-0.017,-0.409, P<0.05), OR (95%CI)were 2.069 (1.206-3.148), 0.803(0.710-0.984), 1.004(0.886-1.431). There was negative correlation between the puncture-related pain score and pain self-efficacy in these patients, r=-0.647, P<0.01. Conclusions Puncture-related pain is common sense in patients with the arteriovenous fistula undergoing maintenance hemodialysis, some factors including the patient gender, using time of arteriovenous fistula and pain self-efficacy influence this feeling.
7.Study on genetic characteristics of VP1 region of Enterovirus 71 strains isolated from Shanghai, China in 2010
Wen ZHU ; Liwen JU ; Lufang JIANG ; Haiyan XIONG ; Qianli WANG ; Qingwu JIANG
Chinese Journal of Infectious Diseases 2011;29(10):583-588
Objective To study the gene characteristics of VP1 region of Enterovirus 71 (EV71) strains isolated from clinical specimens of children with hand-foot-and-mouth disease(HFMD) in Shanghai in 2010.MethodsEighteen EV71 isolates were selected from different periods of year 2010,including strains isolated from fatal cases and non-fatal cases.Complete VP1 gene (891nucleotides) of the eighteen EV71 isolates were amplified and sequenced,and then compared with that of genotype A,B,C reference EV71 strains in GeneBank by homogeneity and phylogenetic tree analyses.ResultsThe nucleotide homogeneities between these 18 Shanghai strains and the representative isolates of genotype A and B were 81.5 % -82.6 % and 83.4 %- 84.2 %,respectively,while the amino acid homogeneities were 94.3 %- 95.0% and 96.6% -97.0%,respectively.The nucleotide and amino acid homogeneities between the 18 Shanghai strains and the representative isolates of genotype C were 87.4%- 99.2% and 98.7% -100.0%,respectively.Of note,the nucleotide and amino acid homogeneities between Shanghai strains and Fuyang EV71strains (representative strain of C4 subtype) appeared to be 97.8%- 99.2% and 99.3%- 100.0%,respectively.The eighteen EV71 Shanghai strains were classified as genotype C,subgenogroup C4 in the phylogenetic tree.There was no remarkable difference in VP1 gene between the strains isolated from fatal cases and non-fatal cases.ConclusionThe EV71 strains isolated from Shanghai belong to subgenogroup C4.
8.Survivability of hand foot mouth disease virus in tap water
Wen ZHU ; Lufang JIANG ; Liwen JU ; Qianli WANG ; Haiyan XIONG ; Qingwu JIANG
Chinese Journal of Infectious Diseases 2011;29(8):455-458
Objective To evaluate the survivability of hand foot mouth disease(HFMD)virus,in tap water for daily use.Methods HFMD viruses were isolated from cases of HFMD in Shanghai and Zhejiang from in 2008.Six isolated strains (five subtype of enterovirus 71 and one coxackie virus)were selected in this study.These viruses were mixed with chloride 1.0 mg/L tap-water and then inoculated into Vero cells.The cytopathic effect (CPE)was checked everyday in order to survey the survivability of each virus strain.The decline of virus survivability was analyzed by scatter diagram.Results These six strains of HMFD virus could survive longer than one month in tap water with initial chloride concentration of 1.0 mg/L and still had celluar infectivity.The survivabilities were varied between viruses isolated from different HFMD cases.Conclusions The survivabilities of enterovirus 71 and coxackie virus stains are quite strong in water.Therefore,the transmission route of water-borne pathogens should be monitored in regions using tap water during HFMD epidemic period.
9.Survey on the enterovirus 71 survival ability on different surfaces under different climate
Yun CAI ; Lufang JIANG ; Yan SHI ; Yuxin LI ; Qianli WANG ; Liwen JU ; Qingwu JIANG
Chinese Journal of Infectious Diseases 2012;30(7):398-401
Objective To evaluate the survival ability of enterovirus 71 (EV71) on different surface and under different climate.Methods Each 1 × 105 tissue culture infective dose 50 (TCID50)EV71 was added on different aseptic surface of plastic,rubber,cloth and wood,respectively.Then these materials were put into biotron (artificial climatic chamber) which could simulate different temperature and moisture.The viruses were recovered after a definite time and then inoculated into Vero cell.The cytopathic effect (CPE) was observed everyday to survey the survival ability of EV71 on different medium surface.Results The recovery rates of EV71 on medium surface ranged from 89 %-93 %.The survival time of EV71 on medium surface varied under different climatic conditions.The longest survival time of the virus was observed under the condition of 20 ℃ as the temperature and 80% as the humidity.After 24 hours of incubation,the infectious titer on plastic surface reduced about 4 lg.After 72 hours of incubation,the infectious titer reduced at least 3.89 lg on cloth and wood surface.Conclusions Temperature and humidity can affect the survival time of EV71 on medium surface,which is longer in the condition of low temperature and high humidity.The survival ability of EV71 on natural cloth and wood surface is better than that on synthetic plastic surface.
10. Research on the influence factors of the carrying system
Lufang ZHANG ; Haojie WANG ; Zhichuan TANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2018;36(9):641-646
Objective:
To investigate surface electromyography signal (sEMG) of the major back muscle group and subjective fatigue assessment under different experimental combinations during walk with weight on both shoulders, as well as the functional status and fatigue property of human muscles.
Methods:
From October to December, 2017, 10 college students were recruited to participate in shoulder weight