1.Current situation and prospects of aircrew survival equipment
Lixiong CHEN ; Yi FEI ; Falin LI ; Lue DENG ; Ying WANG ; Hailiang ZHOU
Chinese Medical Equipment Journal 1989;0(01):-
Aircrew survival equipment is for pilots' survival when they have to parachute or land in various bad situations. Introduction relating to survival equipment is given including development course, sorts, carrying methods, its role in aviation survival and its development tendency. It is indicated that survival equipment will still play an important role in aviation survival within quite a time and it is also imperative to perfect aircrew survival equipment system from improving its performance, increasing its tactical using background and improving its supply system of ordering goods.
2.Recent advances in andrology-related stem cell research.
Ching-Shwun LIN ; Zhong-Cheng XIN ; Chun-Hua DENG ; Hongxiu NING ; Guiting LIN ; Tom F LUE
Asian Journal of Andrology 2008;10(2):171-175
Stem cells hold great promise for regenerative medicine because of their ability to self-renew and to differentiate into various cell types. Although embryonic stem cells (BSC) have greater differentiation potential than adult stem cells, the former is lagging in reaching clinical applications because of ethical concerns and governmental restrictions. Bone marrow stem cells (BMSC) are the best-studied adult stem cells (ASC) and have the potential to treat a wide variety of diseases, including erectile dysfunction (ED) and male infertility. More recently discovered adipose tissue-derived stem cells (ADSC) are virtually identical to bone marrow stem cells in differentiation and therapeutic potential, but are easier and safer to obtain, can be harvested in larger quantities, and have the associated benefit of reducing obesity. Therefore, ADSC appear to be a better choice for future clinical applications. We have previously shown that ESC could restore the erectile function of neurogenic ED in rats, and we now have evidence that ADSC could do so as well. We are also investigating whether ADSC can differentiate into Leydig, Sertoli and male germ cells. The eventual goal is to use ADSC to treat male infertility and testosterone deficiency.
Adult Stem Cells
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Animals
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Cell Transplantation
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Embryonic Stem Cells
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Erectile Dysfunction
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therapy
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Humans
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Infertility, Male
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therapy
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Male
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Research
3.Culture and identification of tumor stem cells from surgically resected colorectal cancer tissues.
Na TANG ; Lue ZHOU ; Zhiqiang CHENG ; Yongjian DENG ; Yanqing DING
Journal of Southern Medical University 2019;39(4):415-421
OBJECTIVE:
To obtain cancer stem cells (CSCs) from surgically resected colorectal cancer specimens and identify their stem cell characteristics.
METHODS:
Colorectal cancer tissue specimen obtained from a patient undergoing radical resection of colorectal cancer were implanted in nude mice, and the xenograft was harvested 1 month later to obtain purified tumor cells by enzyme digestion and adherent culture. The CSCs were screened by limiting dilution method and serum-free culture to identify their phenotypes. Soft agar colony assay was used to assess the proliferative ability of the CSCs and human colorectal cancer cell line SW480. The tumorigenic ability of the isolated CSCs and SW480 cells was evaluated by observing their subcutaneous tumor formation in nude mice. Western blotting and immunofluorescence assay were used to detect the immunophenotype of the CSCs and SW480 cells.
RESULTS:
The primary cultured CSCs from clinical specimens of colorectal cancer underwent differentiation in the presence of serum in the culture. Soft agar colony formation assay showed that the CSCs had a colony formation rate above 50%, significantly higher than the rate of colorectal cancer SW480 cells (4.41%; < 0.01). In nude mice, subcutaneous injection of 500 CSCs was sufficient to result in subcutaneous tumor formation, while the injection of 500 SW480 cells failed to form any subcutaneous tumors. The CSCs expressed CD133 and CD44 but not CK7, while SW480 cells expressed CK7 but not CD133 or CD44.
CONCLUSIONS
CSCs can be derived by primary culture of cancer cells obtained from surgically resected colorectal cancer tissue followed by serum-free culture, and the CSCs obtained have self-renewal and differentiation abilities.
Animals
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Cell Culture Techniques
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Cell Differentiation
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Cell Line, Tumor
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Colorectal Neoplasms
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Humans
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Mice
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Mice, Nude
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Neoplastic Stem Cells