Objective To compare the evaluation effect ,clinical feasibility and influencing factors of two methods on comparative analyzing the blood glucose measurement of glucose meter and automatic analyzer .Methods 61 diabetic or non-diabetic patients were recruited in this study .All subjects were drawn 2 mL fasting venous blood using EDTA-K2 tubes following an 8~12 h over-night fast .One drop of venous whole blood was used to measure the blood glucose concentration (VBG)with the meter and the rest of blood was centrifuged for detecting the plasma glucose (VPG)by automatic analyzer (HITACHI-7600) .The capillary blood was taken by finger stick to test blood glucose(CBG)using the same meter within 5 min after the venous blood collection .Taking the VPG as the reference ,to calculate the respective bias of VBG and CBG .Correlation of VPG with VBG or CBG was analyzed .The clinical acceptance of glucose meter readings was evaluated by Parkes error grid analysis .Results Both VBG and CBG measured by Accu-Chek Performa glucose meter were highly correlated with VPG ,with correlation coefficient 0 .991 and 0 .989 respectively .All the measurements satisfactorily met the GB/T 19634-2005 criteria and all the results were clinically accepted .Conclusion To com-pare the results of blood glucose measured by glucose meter and automatic analyzer ,venous blood sample is recommended because of the same blood resource ,convenience of operation and possibility of making blood samples with extremely low or high glucose concentration .

Object To study the chemical constituents from Epimeredi indica and search for the natu-ral compounds with bioactivities. Methods Three compounds were isolated by a combination of RP-18and silica gel column chromatographies, and their structures were identified by spectral methods. ResultsA novel 2- (3-methoxy-4-hydroxy) phenyl-ethanol 1-O-a-L- [(1→3 )-rhamnopyranosyl- 6-feruloyl] gluco-side ( I ) along with known 2-(3, 4-dihydroxy) phenyl ethanol 1-O-?-L-[(1→3)-rhamnopyranoside-4-O-caffeoyl-] glucoside (verbascoside) ( Ⅱ ), and 2-(3, 4-dihydroxy) phenyl ethanol (1→1) (2→2)-[(1→3)-rhamnopyranoside-4-O-caffeoyl glucoside (oraposide) (Ⅲ) were isolated from E. indica. ConclusionCompound I is a new compound named as epimeredinoside A and compounds Ⅱ and Ⅲ are isolated fromthis plant for the first time.

AIM: To study the correlation between the release rate in vitro and the absorption in vivo of Shenshen sustained-release Capsule(SSRC phenylethanoid glycosides). METHODS: Release rate of SSRC in vitro was tested with D-800LS dissolution tester. 8 rats were randomly divided into two groups. One group was orally fed on SSRC. Plasma levels were determined by UV and the absorption fraction was calculated according to Wagner-Nelson's formula. RESULTS: The correlation coefficient between the release rate in vitro and the absorption fraction in vivo was 0.9654 . CONCLUSION: There is a significant correlation between release rate in vitro and absorption in vivo to SSRC.

Objective To investigate the association of cytotoxic T lymphocyte associated antigen 4(CTLA 4) gene polymorphism with type 1 diabetes in Chinese Han population.Methods The A/G phenotype at position 49 of the CTLA 4 gene exon 1 was determined by polymerase chain reaction restriction fragment length polymorphism(PCR RFLP)method in 33 typical type 1 diabetes patients,57 latent autoimmune diabetes in adults(LADA) patients and 84 healthy control subjects of Chinese Han.Results The frequency of the CTLA 4/G 49 phenotype was significantly higher in type 1 diabetes patients than in control subjects(55.6% vs 36.9%, respectively, P =0.0005),but there was no significant difference between typical type 1 DM and LADA groups. Neither the presence nor the absence of G 49 allele influenced the occurrence of islet autoantibody(ICA) and glutamate decarboxylase antibody(GADAb).Conclusion The polymorphism of CTLA 4 gene exon1 confers susceptibility to type 1 diabetes. This association is independent of ICA and GADAb.

Child ; Hemofiltration ; Humans ; Male ; Paraquat ; poisoning ; Poisoning ; therapy ; Treatment Outcome

Objective To observe the efficiency of infection of adenovirus containing hepatocyte growth factor(Ad-HGF) on adipose derived stem cells and to prove whether the valid HGF can appear after infection and the multiplicity of infection. Methods We use the digestion separation method and the attachingwall characteristic of the adipose-derived stem cells to separate the human adipose-derived stem cells. Adipose-derived stem cells were infected by the vector of adenovirus (Ad-GFP) which carries the GFP gene,and the GFP acts as the indicating gene to determine the infection efficiency of recombinant adenovirus to adipose- derived stem cells. HGF-ELISA was used to detect HGF as expression-secretion. Results The adherent cells displayed themselves as fibroblast in morphology. The primary cultured cells fusion can arrive to 70% - 80% in 7 - 10 days. The infected HGF can be highly expressed in 48hours. Conclusion Adenovirus can meditate the expression of HGF gene in adipose-derived stem cells effectively.

BACKGROUND: Cell labeling and nuclear magnetic resonance can non-invasively in vivo label the region, existing mode and some bionomics of transplanted neural stem cells (NSCs). OBJECTIVE: To observe the outcome of superparamagnetic iron oxide in vitro labeled human NSCs. DESIGN, TIME AND SETTING: The cytology, in vitro, observation study was performed at the Laboratory of Department of Pediatrics, Navy General Hospital of Chinese PLA from December 2006 to June 2007. MATERIALS: Aborted human embryo was provided by Navy General Hospital of Chinese PLA. Superparamagnetic iron oxide (Lot number 97060601) was produced by Advanced magnetics,inc., USA. METHODS: Monoplast suspension was isolated from human embryo hippocampus using the mechanical method, and in vitro incubated in NSC medium, supplemented with epidermal growth factor and basic fibroblast growth factor. 11.2 g/L superparamagnetic iron oxide was diluted into 28 mg/L using NSC medium, mixed with 1.0 mg/L polylysine (8 ?L), and then made into superparamagnetic iron oxide-polylysine composite labeled medium. NSC spheres with active proliferation were obtained, made into single cell suspension (1?109/L), and then treated with serum-free medium containing superparamagnetic iron oxide. One week later, various cytokines were removed, and 5% fetal bovine serum was used for 24 hours to induce the NSC differentiation. MAIN OUTCOME MEASURES: Prussian blue staining was utilized to determine marking positive rate. Immunofluorescence staining was applied to detect glial fibrillary acidic protein and neurofilament expression. RESULTS: Superparamagnetic iron oxide labeled NSCs were yellow, and the speed of clone formation was not stepped down compared with the non-labeled cells. 20 hours following superparamagnetic iron oxide labeling, blue iron particles in NSCs cytoplasm were found, with the positive rate of 90%. Following induction, superparamagnetic iron oxide labeled NSCs were positive for glial fibrillary acidic protein and neurofilament. CONCLUSION: Superparamagnetic iron oxide can highly effectively label NSCs in vitro, and not affect biological features following labeling. NSCs can normally amplify and orientedly differentiate into neurons and astrocytes.

AIM: To study Cordyceps sinesis and its cultured mycelia by HPLC.METHODS: An HPLC established for the fingerprints of cordyceps sinensis and its cultured mycelia was performed on an Agilent C18(4.6 mm ? 25 mm,5 ?m)as column and acetonitrile-0.1% phosphoric acid solution in gradient as mobile phase.The flow rate was 0.5 mL/min,and the detection wavelength was set at 260 nm.RESULTS: There were 6 common peaks in HPLC fingerprint of Cordyceps corensis and its cultured mycelia,three of which were determined as uracil,adenosine,cordycepin.CONCLUSION: The method can be used for the study of fingerprints of cultured mycelia.

To investigate the prognosis relevant factors of endometrial carcinoma. Clinical pathological materials of 68 cases of endometrial carcinoma were analyzed. The 5 year survival rate of ≥40 years old women with endometrial carcinoma was slightly less than that of the younger women, while menopause showed no influence on the survival rate .The 5 year survival rate of patients with term parity ≥2 was 92 7%( P 0 05). Therefore, surgical method ,clinical staging ,invasion depth of uterine muscle and number of term parity are the important factors which influence the prognosis of endometrial carcinoma.

OBJECTIVE:To study the therapeutic window of peak blood concentration at different time in domestic renal transplant recipients2h after administration with cyclosporine A(CsA).METHODS:The valley bottom levels(C 0 )and the peak levels(C 2 )were determined simultaneously by fluorescence polarization immunoassay(FPIA)in92patients2h after oral administration with CsA,the rejection and the hepatic and renal drug toxicities were observed.RESULTS:The recommended therapeutic window concentration of CsA(C 2 )in Chinese renal transplant recipients was1000～1300?g/L within the first month,950～1250?g/L within the second to third month,900～1100?g/L within the fourth to sixth month,750～1000?g/L within the seventh to twelfth month,600～800?g/L from the twelfth month after renal transplantation.CONCLUSION:Within the above therapeutic window concentration range,CsA is of ideal immuno-suppressive action meanwhile it can less rejection and minimize the hepatic and renal drug toxicities.