1.Evaluation of 2 methods for comparing the glucose measurement between glucose meter and automatic analyzer
International Journal of Laboratory Medicine 2014;(18):2516-2518
Objective To compare the evaluation effect ,clinical feasibility and influencing factors of two methods on comparative analyzing the blood glucose measurement of glucose meter and automatic analyzer .Methods 61 diabetic or non-diabetic patients were recruited in this study .All subjects were drawn 2 mL fasting venous blood using EDTA-K2 tubes following an 8~12 h over-night fast .One drop of venous whole blood was used to measure the blood glucose concentration (VBG)with the meter and the rest of blood was centrifuged for detecting the plasma glucose (VPG)by automatic analyzer (HITACHI-7600) .The capillary blood was taken by finger stick to test blood glucose(CBG)using the same meter within 5 min after the venous blood collection .Taking the VPG as the reference ,to calculate the respective bias of VBG and CBG .Correlation of VPG with VBG or CBG was analyzed .The clinical acceptance of glucose meter readings was evaluated by Parkes error grid analysis .Results Both VBG and CBG measured by Accu-Chek Performa glucose meter were highly correlated with VPG ,with correlation coefficient 0 .991 and 0 .989 respectively .All the measurements satisfactorily met the GB/T 19634-2005 criteria and all the results were clinically accepted .Conclusion To com-pare the results of blood glucose measured by glucose meter and automatic analyzer ,venous blood sample is recommended because of the same blood resource ,convenience of operation and possibility of making blood samples with extremely low or high glucose concentration .
2.Phenethyl alcohol glycosides in Epimeredi indica
Chinese Traditional and Herbal Drugs 1994;0(12):-
Object To study the chemical constituents from Epimeredi indica and search for the natu-ral compounds with bioactivities. Methods Three compounds were isolated by a combination of RP-18and silica gel column chromatographies, and their structures were identified by spectral methods. ResultsA novel 2- (3-methoxy-4-hydroxy) phenyl-ethanol 1-O-a-L- [(1→3 )-rhamnopyranosyl- 6-feruloyl] gluco-side ( I ) along with known 2-(3, 4-dihydroxy) phenyl ethanol 1-O-?-L-[(1→3)-rhamnopyranoside-4-O-caffeoyl-] glucoside (verbascoside) ( Ⅱ ), and 2-(3, 4-dihydroxy) phenyl ethanol (1→1) (2→2)-[(1→3)-rhamnopyranoside-4-O-caffeoyl glucoside (oraposide) (Ⅲ) were isolated from E. indica. ConclusionCompound I is a new compound named as epimeredinoside A and compounds Ⅱ and Ⅲ are isolated fromthis plant for the first time.
3.Correlation between release rate in vitro and adsorption in vivo of Shenshen sustained-release Capsule
Chinese Traditional Patent Medicine 1992;0(04):-
AIM: To study the correlation between the release rate in vitro and the absorption in vivo of Shenshen sustained-release Capsule(SSRC phenylethanoid glycosides). METHODS: Release rate of SSRC in vitro was tested with D-800LS dissolution tester. 8 rats were randomly divided into two groups. One group was orally fed on SSRC. Plasma levels were determined by UV and the absorption fraction was calculated according to Wagner-Nelson's formula. RESULTS: The correlation coefficient between the release rate in vitro and the absorption fraction in vivo was 0.9654 . CONCLUSION: There is a significant correlation between release rate in vitro and absorption in vivo to SSRC.
4.The relationships between CTLA-4 gene exon 1 A/G~(49) polymorphism and type 1diabetes mellitus
Luan WANG ; Ruixin MA ; Fei WANG
Chinese Journal of Diabetes 1994;0(01):-
Objective To investigate the association of cytotoxic T lymphocyte associated antigen 4(CTLA 4) gene polymorphism with type 1 diabetes in Chinese Han population.Methods The A/G phenotype at position 49 of the CTLA 4 gene exon 1 was determined by polymerase chain reaction restriction fragment length polymorphism(PCR RFLP)method in 33 typical type 1 diabetes patients,57 latent autoimmune diabetes in adults(LADA) patients and 84 healthy control subjects of Chinese Han.Results The frequency of the CTLA 4/G 49 phenotype was significantly higher in type 1 diabetes patients than in control subjects(55.6% vs 36.9%, respectively, P =0.0005),but there was no significant difference between typical type 1 DM and LADA groups. Neither the presence nor the absence of G 49 allele influenced the occurrence of islet autoantibody(ICA) and glutamate decarboxylase antibody(GADAb).Conclusion The polymorphism of CTLA 4 gene exon1 confers susceptibility to type 1 diabetes. This association is independent of ICA and GADAb.
5.Successful rescue of a child with paraquat poisoning by using blood purification.
Tao LI ; Jiang-wei LUAN ; Xu WANG
Chinese Journal of Pediatrics 2010;48(11):870-871
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Hemofiltration
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Paraquat
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poisoning
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Poisoning
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therapy
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Treatment Outcome
6.Differences in endocrine and metabolic parameters in polycystic ovary syndrome patients with and without subclinical hypothyroidism
Yanni XIE ; Luan WANG ; Ruixin MA
Chinese Journal of Endocrinology and Metabolism 2014;30(3):187-191
Objective To compare the endocrine and metabolic parameters between patients suffering from polycystic ovary syndrome (PCOS) with or without subclinical hypothyroidism.Methods One hundred and fifty-six PCOS patients and 47 healthy controls were enrolled.Height,weight,waist circumference,and hip circumference were measured and recorded.Fasting blood samples were collected for measuring luteinizing hormone (LH),follicular stimulating hormone (FSH),total testosterone,prolactin,estrogen,sex hormone binding globulin (SHBG),thyroidstimulating hormone (TSH),free triiodothyronine (FT3),free thyroxine (FT4),blood glucose,serum insulin,triglycerides,total cholesterol,low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol (HDL-C).Body mass index,waist-to-hip ratio,LH-to-FSH ratio,and free androgen index (FAI) were calculated.Homeostasis model assessment of insulin resistance (HOMA-IR) and β cell function (HOMA-β) were assessed.All patients underwent ultrasound for diagnosing polycystic ovary.All the patients were subdivided into two groups in terms of TSH value:subclinical hypothyroidism group with TSH level ≥4.2 μIU/ml and non-subclinical hypothyroidism group with TSH level<4.2 μIU/ml.The differences in endocrine and metabolic parameters were compared between two subgroups.Results Compared with healthy controls,the PCOS patients had significantly higher body mass index,waist-to-hip ratio,LH,LH-to-FSH ratio,total testosterone,FAI,TSH,and lower SHBG (all P<0.05).The other indexes were not different between two groups (P>0.05).24.4% PCOS patients were diagnosed as cases of subclinical hypothyroidism.TSH,prolactin,and triglycerides levels were higher in PCOS patients with subclinical hypothyroidism than those without (P<0.01).Whereas estrogen,FT3,FT4 were significantly lower(P<0.01).The differences of other parameters were not significant between two groups(P>0.05).The results of correlation analysis and TSH quartiles showed no significant linear correlation between TSH and sex hormones,lipids,insulin resistance indices (P>0.05).Conclusions The incidence of subclinical hypothyroidism in all PCOS patients is 24.4%.Prolactin and triglycerides levels were higher in PCOS patients with subclinical hypothyroidism than those without.Whereas estrogen,FT3,FT4 were significantly lower.
7.Application of microcapsulated cells or encystation materials for treating diabetes mellitus
Yao WANG ; Jian LUAN ; Jietao ZHANG
Chinese Journal of Tissue Engineering Research 2009;13(47):9349-9352
OBJECTIVE: To systematically evaluate the application of microcapsuled cell or encystation materials for treating diabetes mellitus at hone or abroad in recent years.METHODS: A computer-based online search of PubMed database was undertaken to identify related articles about the microcapsulated cell or encystation material for treating diabetes mellitus published from 1980 to 2009 by using the key words of "microcapsulation, islet, transplantation" in English. Meanwhile, we search CNKI for relevant articles published between 1999 and 2009 with the same key words in Chinese. The related randomized, controlled, and clinical studies were collected according to conclusion and exclusion criteria.RESULTS: Among 25 articles, there were 46 patients with type I diabetes mellitus, 106 rats, 20 pigs, and 25 monkeys. The experimental results showed that both the microcapsulated islets and non-microcapsulated islets could secrete insulin and decrease blood glucose level. The normal blood glucose level with microcapulated islets could be maintained for a long time.Thus, microcapsule had a great immune isolation reaction.CONCLUSION: After transplantation, microencapsulated islet cells could improve and adjust abnormal glycometabolism of patients with diabetes mellitus; furthermore, the immunoisolation effect of microcapsule could eliminate or relieve the immunological rejection of receptors to allograft or xenograft, while it could also relieve or eliminate dependence on immunosuppressive drugs. Appropriate material of microcapsule, reasonable process, advanced equipment, advanced separation and purification technologies of islet, and suitable site for transplantation could improve the function of artificial islet cell, enhance the anti-machinery and chemical strength of microencapsuiated islet, improve biocompatibility, and prolong survival time.
8.Determination of the Related Substances in Azithromycin Tablet by HPLC-Electrochemical Detector
Jie LUAN ; Guoqing ZHOU ; Haixia WANG
China Pharmacy 2007;0(25):-
OBJECTIVE: To determine the content of the related substances in azithromycin tablet by HPLC-electrochemical detector.METHODS: The column was Gamma ARP-1/P.The mobile phase consisted of 0.014 mol?L-1 dipotassium hydrogen phosphate/0.020 mol?L-1 sodium hydroxide(pH was adjusted to 11 by phosphoric acid and sodium hydroxide)-acetonitrile(71∶29) at a flow rate of 0.7 mL?min-1.The column temperature was 40 ℃.The injection volume was 50 ?L.Glass carbon working electrode was set at +900 mV.RESULTS: The linear ranges of erythromycin peroxide,deoxyazithromycin,azithromycin impurity A,and N-demethlation azithromycin were 0.15~1.80 ?g(r=0.999),0.09~1.08 ?g(r=0.999),0.23~2.76 ?g(r=0.998) and 0.30~3.60 ?g(r=0.998),respectively.The average recoveries were 98.9%(RSD=1.1%),94.6%(RSD=5.4%),103.4%(RSD=3.4%) and 96.2%(RSD=4.3%),respectively.The contents of the related substances were all below 0.7%.CONCLUSION: The established method is reproducible,sensitive and reliable,and applicable for the detection of the related substances in azithromycin tablets.
9.Repair of bony defective nonunion of humeral condyle using autologous platelet-rich-plasma combined with iliac autograft
Tao SUN ; Jianhang WANG ; Jingjie LUAN
Chinese Journal of Orthopaedic Trauma 2016;18(6):498-502
Objective To investigate the clinical effectiveness of autologous platelet-rich-plasma (PRP) combined with iliac autograft in reconstruction of bony defective nonunion of humeral condyle.Methods The 27 patients who had been admitted to our department from January 2004 to June 2015 for bony defective nonunion of humeral condyle were analyzed retrospectively.Of them,15 underwent reconstruction with PRP combined with iliac autograft (experimental group) and 12 reconstruction with only iliac autograft (control group).The preoperative demographic data of the 2 groups were not significantly different (P > 0.05).The 2 groups were compared in terms of operation time,hospital stay,bony union time,range of motion of the elbow,and modified Cassebaum scoring.Results The patients were followed up for 12 to 90 months (average,24.6 months).No such complications were observed as displacement of bone blocks,loosening or breakage of implants,re-nonunion,malunion,delayed ulnar neuritis,or olecranon nonunion.There were no statistic differences between the 2 groups regarding operation time,hospital stay,range of motion of the elbow and modified Cassebaum scoring (P > 0.05).The clinical healing time (4.2 ± 1.3 months) and bony healing time (4.8 ± 1.4 months) for the experimental group were significantly shorter than for the control group (6.0 ± 1.1 months and 6.2 ± 1.0 months,respectively) (P < 0.05).Conclusion Autologous PRP combined with iliac autograft can speed up the healing of bony defective nonunion of humeral condyle,promoting functional recovery of the elbow.
10.The association of cytotoxic T lymphocyte-associated antigen 4 gene polymorphism with type 1 diabetes mellitus and autoimmune thyroid diseases in Chinese Han population
Luan WANG ; Hongwei YU ; Shenli YAN
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
Objective To investigate the association of cytotoxic T lymphocyte associated antigen 4 (CTLA 4) gene polymorphism with type 1diabetesmellitusandautoimmunethyroiddiseases. Methods The A/G phenotype at position 49 of the CTLA 4 gene exon 1 was determined by PCR RFLP method in 33 classic type 1 diabetes patients, 57 latent autoimmune diabetes in adults (LADA) patients, 122 autoimmune thyroid disease patients and 84 healthy control subjects of Chinese Han. Results The frequency of the CTLA 4 G phenotype was significantly higherintype1diabetespatientsthanthatincontrol subjects (55.6% vs 36.9%, P=0.0005). Neither the presence nor the absence of G allele influenced the occurrence of islet autoantibody (ICA) and glutamate decarboxylase antibody (GADA). The strong association of the CTLA 4 G allele with AITDs was showed in our study (66.4% vs 36.9%, P